ISSN:
1573-5036
Schlagwort(e):
Hydrolysis
;
Plant roots
;
Phosphatases
;
Phosphorus fertilizers
;
Polyphosphates
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
Notizen:
Summary The hydrolysis of seven linear oligomers (P2, P3, P5, P15, P25, P35 and P65) and one cyclic polyphosphate, trimetaphosphate (TMP), by corn (Zea mays) roots was investigated. In these experiments, corn-root homogenate or intact roots were incubated in a polyphosphate solution containing 1 mM polyphosphate or 50 mg P/L, respectively, and the amount of orthophosphate produced was determined. Results showed that the optimal pH value for hydrolysis of P3, P5, and TMP by corn-root homogenate was 5.0, whereas for the hydrolysis of P2, P15, P25, P35 and P65, it was 6.0. The rate of polyphosphate hydrolysis by cornroot homogenate was temperature dependent up to the point of enzyme inactivation (〉50°C). Nonsterile intact roots showed higher rates of hydrolysis than sterile roots, especially with P2. The hydrolysis of all oligomers by sterile and nonsterile intact roots was very slow during the first 18h at 30°C, but increased rapidly after 18h with the oligomers P≦25. The oligomers P35 and P65 were quite resistant to hydrolysis by sterile and nonsterile roots after 48h incubation at 30°C. An experiment with sterile intact roots in pyrophosphate solution suggested that pyrophosphatase was induced in corn roots in the presence of its substrate. The order of hydrolysis rates of the oligomers by intact sterile corn roots was: P2〉P3〉P5〉 TMP〉P15〉P25〉P35〉P65.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02374348
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