ISSN:
1573-5060
Keywords:
Agrobacterium tumefaciens
;
biolistics
;
co-suppression
;
co-transformation
;
electroporation
;
epistasis
;
gene silencing
;
somaclonal variation
;
transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary The DNA delivery systems which are routinely used to introduce genes into crop plants are Agrobacterium tumefaciens, electroporation and particle bombardment. The differences and similarities between these different transformation techniques are outlined. The influence of the cell biological approach, and more specifically the impact of the state of the plant cell at the moment of transformation, on the genotype and phenotype of the regenerated transgenic plant is analysed. In this respect phenomena such as position effects, gene silencing, co-suppression, epistasis, co-transformation and somaclonal variation are discussed. The relevance of these factors for plant breeders is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00023461
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