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  • 1995-1999  (40,574)
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  • Chemistry  (61,391)
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  • transformation
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  • 1
    ISSN: 1432-0975
    Keywords: Key words Otolith ; Chemistry ; ICP-MS ; Stock discrimination ; Epinephelus striatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract  We examined the utility of otolith minor and trace element chemistry, assayed with inductively coupled plasma mass spectrometry (ICP-MS), as a means of delineating population structure in the Nassau grouper (Epinephelus striatus). We characterized the elemental composition of otoliths collected in 1993 from three locations in Exuma Sound, Bahamas and from Glover Reef, Belize in 1995. A single location in Exuma Sound was sampled in 1994 to test temporal variability in otolith composition. Five elements (Ca, Zn, Sr, Ba and Pb) were routinely detected, at levels significantly above background, by solution-based ICP-MS. Results from analysis of variance of elemental data, expressed as a ratio to Ca, indicated that there were no significant differences among the Exuma locations for any element, but significant variability was found between Glover Reef and the pooled Exuma localities for Zn/Ca, Sr/Ca and Ba/Ca ratios. Significant inter-annual differences at one Exuma Sound location was restricted to Ba/Ca ratios. Discriminant function analysis correctly classified 86% and 95% of the Belize and pooled Exuma sites, respectively. Otoliths from Belize were characterized by low Zn/Ca and high Ba/Ca and Pb/Ca ratios compared to otoliths from fish collected in Exuma Sound. Although differences in Ba levels may be related to upwelling at Glover Reef, more data are needed to definitely link otolith composition with regional differences in water chemistry.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology reporter 17 (1999), S. 323-331 
    ISSN: 1572-9818
    Keywords: Agrobacterium ; modular vector ; transformation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of thermal analysis and calorimetry 56 (1999), S. 1133-1140 
    ISSN: 1572-8943
    Keywords: branched diamine ; melting ; polyamides ; polymorphism ; transformation ; WXRD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Our X-ray work of Dytek®-A, 2-methyl-pentamethylenediamine, containing polyamides shows polymorphism, whereas the polyamides with linear diamines do not. The polyamide of Dytek®-A and dodecanedioic acid, MPMD-12, is singled out for discussion and compared with the unbranched analogs of polyamides 6,12 and 5,12. Due to the presence of the -CH3 side group in the 2-position of the diamine, the polyamide MPMD-12 exhibits two stable crystal conformations. The new δ polymorph is not seen in linear polyamides 6,12 and 5,12. Studies by DSC polyamide MPMD-12 clearly illustrates at least two crystal forms, γ and δ, coexisting over a wide temperature range, and the isolation of each phase is possible by controlling temperature and time. The DMA modulus in the temperature region between the glass transition (or alpha relaxation) and melting transition shows strong dependence on the thermal history as demonstrated in a study of crystallization kinetics.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-9368
    Keywords: Agrobacterium tumefaciens ; ß‐glucuronidase ; lamiaceae ; lavandin ; neomycin phosphotransferase II ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lavandin (Lavandula x Emeric ex Loiseleur) is an aromatic plant, the essential oil of which is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. The qualitative or quantitative modification of its terpenes‐containing essential oil by genetic engineering could have important scientific and commercial applications. In this study, we report the first Agrobacterium tumefaciens‐mediated gene transfer into lavandin. The transformation protocol was optimized by lengthening precultivation and cocultivation periods and by testing five different bacterial strains. We obtained transformed callus lines at a frequency of 40–70 with strains AGL1/GI, EHA105/GI and C58/GI. Transgenic shoots were regenerated from these kanamycin resistant calli and rooted on selective medium with 150 mg l-1 kanamycin. The final percentage of transgenic plants obtained varied from 3 to 9, according to the strain used, within 6 months of culture. The presence of the introduced β‐glucuronidase and neomycin phosphotransferase II genes was shown both by PCR and Southern blot analysis. Transgene expression was investigated using histoenzymatic β‐glucuronidase assays, leaf callus assays and RT‐PCR. Results showed that both β‐glucuronidase and neomycin phosphotransferase II genes were expressed at a high level in at least 41 of the transgenic plants regenerated. This efficient transformation strategy could be used to modify some genetic traits of lavandin (flower colour, pathogens resistance) and to study the biosynthesis of the major monoterpene components of its essential oil (linalool, linalyl acetate, camphor and 1,8‐cineole).
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-9368
    Keywords: Lactuca sativa ; Agrobacterium tumefaciens ; bialaphos ; phosphinothricin acetyltransferase ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resistance to bialaphos, a broad-spectrum herbicide, was introduced into Lactuca sativa cv. Evola by Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strains 0310 and 1310, both carrying the bialaphos resistance (bar) and neomycin phosphotransferase (nptII) genes, were used for transformation. Primary transformants were selected on kanamycin sulphate-supplemented shoot regeneration medium. Integration of both transgenes was confirmed by non-radioactive Southern hybridisation. The hypervirulent plasmid ToK47 in A. tumefaciens strain 1310 generated multiple insertions of T-DNA in some transgenic plants; the absence of pToK47 (strain 0310) resulted in single gene inserts in all plants tested. Resistance to glufosinate ammonium was observed in axenic seedlings grown on medium supplemented with the herbicide at 5 mg l−1 and in glasshouse-grown plants sprayed with the compound at 300 mg l−1. Stable expression of the bar gene was observed in R2 generation plants. The kanamycin resistance of R1 seedlings was observed by germinating seeds on medium supplemented with 200 mg l−1 kanamycin sulphate. The presence of NPTII protein and PAT enzyme activity were demonstrated by ELISA and PAT enzyme assay respectively. Transgenes segregated in a Mendelian fashion in some plant lines in the R1 generation; herbicide resistance also segregated in the expected ratio in the R2 generation in most transgenic lines. This study confirmed that an agronomically important transgene can be integrated and stably expressed over several generations in lettuce.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-9788
    Keywords: Triticum turgidum L. var. durum ; pasta wheat ; transformation ; seed protein modification ; flour quality improvement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Particle bombardment has been used to transform three cultivars (L35, Ofanto, Svevo) and one breeding line (Latino × Lira) of durum wheat (Triticum turgidum L. var. durum). These varieties were co-transformed with plasmids containing selectable and scorable marker genes (bar and uidA) and plasmids containing one of two high-molecular-weight (HMW) glutenin subunit genes (encoding subunits 1Ax1 or 1Dx5). Ten independent transgenic lines were recovered from 1683 bombarded scutella (transformation efficiency thus 0.6%). Five lines expressed either subunit 1Dx5 or 1Ax1 at levels similar to those of endogenous subunits encoded on chromosome 1B. To identify the effects of the transgenes on the functional properties of grain, three lines showing segregation for transgene expression were used to isolate sibling T2 plants which were null or positive for the transgene product. Analysis of these plants using a small-scale mixograph showed that expression of the additional subunits resulted in increased dough strength and stability, demonstrating that transformation can be used to modify the quality of durum wheat for bread and pasta making.
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  • 7
    ISSN: 1572-9788
    Keywords: Cyamopsis tetragonoloba ; β-lactamase inhibitor ; sulbactam ; transformation ; transgene stability ; transgenic guar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A procedure for transformation of the large-seeded endospermous legume guar (Cyamopsis tetragonoloba L.) and a study on transmission of the transgenes to offspring generations are presented. Using Agrobacterium tumefaciens with a T-DNA construct harbouring a β-glucuronidase gene (uidA) and a neomycin phosphotransferase gene (nptII), maximum transformation frequencies of cotyledonary explants were obtained using 145 mg/l kanamycin sulfate as selective agent. Carbenicillin and cefotaxime, used for the elimination of Agrobacterium after co-culture, displayed considerable toxicity to guar tissues but replacing most of these β-lactams by the non-phytotoxic β-lactamase inhibitor sulbactam as well as addition of thidiazuron and silver thiosulfate increased transformation frequencies up to 10-fold in total. The presence of the transgenes in the primary transformants was demonstrated by genomic DNA analysis of GUS-positive shoots. Chimaeric plants (5–10%) were identified by GUS analysis at the flowering stage and were discarded. Analysis of the R1 offspring from 17 independent transformants showed that in 41% of those, the uidA gene(s) was expressed and stably inherited consistent with Mendelian genetics. This was also found for the R2 and R3 generations of single copy transformants. On the other hand, a large proportion (47%) of the primary transformants gave R1 offspring in which 100% of the plants were GUS-negative. Analysis of these plants by PCR revealed that, at least, most of the transgene sequences were absent, suggesting that they had not been transmitted from the parent transformants. This occurred at similar high frequencies (40–50%) irrespective of the estimated copy number of the transgenes. Thus, major parts of the transgenes, even when present in multiple copies, displayed aberrant transmission, at a high frequency, in the process of going from the primary transformants to the first offspring generation.
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  • 8
    ISSN: 1572-9788
    Keywords: alternative oxidase ; antisense ; male-sterility ; tapetum-specific promoter ; tobacco ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The alternative oxidase of plant mitochondria is the terminal oxidase of the cyanide-insensitive respiratory pathway and is encoded by a nuclear gene. A 1 kb genomic fragment including exon 3 of the alternative oxidase was amplified by PCR from the genome of Arabidopsis thaliana. This fragment was connected to a tapetum-specific promoter in the antisense orientation and then introduced into tobacco. The pollen viability in three transgenic plants ranged from 2% to 60%. The reduced pollen viability cosegregated with the transgene in a selfed progeny. Immunolocalization of alternative oxidase protein in the immature flower bud section indicated that expression of alternative oxidase protein in tapetum of the transgenic plant was much lower than that of the non-transformant. The histological observation and protein gel-blot analysis showed that the development of pollen grains in the transgenic plant did not progress after the degradation of the tapetum, and the amount of alternative oxidase in pollen grains of the transgenic plant became lower than that of the non-transformant. These results suggested that the alternative oxidase activity in the tapetum has a significant effect on the pollen development.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Computer supported cooperative work 8 (1999), S. 63-93 
    ISSN: 1573-7551
    Keywords: activity theory ; action ; transformation ; expansive learning ; intervention ; visibilization ; health care ; medical records
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Work is commonly made visible along two dimensions: the linear and the socio-spatial. Both are limited to depicting work in terms of relatively discrete actions. Activity theory introduces the crucial distinction between collective activity systems and individual actions. Expansive visibilization of collective activity systems offers a powerful intervention methodology for dealing with major transformations of work. The linear and the socio-spatial dimensions of work actions are seen in the broader perspective of a third, developmental dimension of work activity. Four steps are identified in a cycle of expansive visibilization, combining activity-level visions and action-level concretizations. The cycle is examined in detail as it unfolded in an intervention study at a children's hospital in Finland. It is concluded that expansive visibilization, driven by contradictions and seeking to reconceptualize the object and motive of work, is not a straightforward process which can be neatly controlled from above. Coherent analytical explanation and goal-setting may come only after the creation and practical implementation of innovative solutions.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plant pathology 105 (1999), S. 221-229 
    ISSN: 1573-8469
    Keywords: mutagenesis ; transformation ; plant disease ; recombination ; plant pathogenic fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Development of molecular techniques for phytopathogenic fungi aims at the identification of fungal genes whose products are essential for successful infection of the host plant. Initial approaches have relied on isolating candidate genes and generating null-mutations by homologous recombination. Unfortunately, the results of this strategy have not been overly successful. This has led to a search for alternatives which allow an unbiased identification of pathogenicity genes. One method, which has proved successful in several systems, is a tagging mutagenesis procedure termed restriction enzyme mediated integration (REMI). In this mini-review we describe this procedure and review its features and results of its use when applied to the identification of fungal genes required for disease development in planta.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 40 (1999), S. 711-717 
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; Arabidopsis thaliana ; binary vector ; T-DNA ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A streamlined mini binary vector was constructed that is less than 1/2 the size of the pBIN19 backbone (3.5 kb). This was accomplished by eliminating over 5 kb of non-T-DNA sequences from the pBIN19 vector. The vector still retains all the essential elements required for a binary vector. These include a RK2 replication origin, the nptIII gene conferring kanamycin resistance in bacteria, both the right and left T-DNA borders, and a multiple cloning site (MCS) in between the T-DNA borders to facilitate cloning. Due to the reduced size, more unique restriction sites are available in the MCS, thus allowing more versatile cloning. Since the traF region was not included, it is not possible to mobilize this binary vector into Agrobacterium by triparental mating. This problem can be easily resolved by direct transformation. The mini binary vector has been demonstrated to successfully transform Arabidopsis plants. Based on this mini binary vector, a series of binary vectors were constructed for plant transformation.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cancer and metastasis reviews 18 (1999), S. 215-230 
    ISSN: 1573-7233
    Keywords: transformation ; tumour ; Frizzled ; Dishevelled ; glycogen synthase kinase-3β
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Wnt signalling is involved in a variety of mammalian developmental processes, including cell proliferation, differentiation and epithelial–mesenchymal interactions, through which they contribute to the development of tissues and organs such as the limbs, the brain, the reproductive tract and the kidney. Wnts are secreted ligands that control cell processes via at least two pathways, one of which, the ‘canonical’ Wnt signalling pathway, operates through the cytosolic stabilisation of a transcriptional co-factor, β-catenin. This is achieved by downregulating the activity of a β-catenin turnover complex. Evidence from tumour expression studies, transgenic animals and in vitro experiments suggests that inappropriate activation of the canonical Wnt signalling pathway is a major feature in human neoplasia and that oncogenic activation of this pathway can occur at many levels. Inappropriate expression of the Wnt ligand and Wnt binding proteins have been found in a variety of human tumours. Further downstream, dysregulation of the β-catenin turnover complex, by loss of the Adenomatous Polyposis Coli or Protein Phosphatase 2A proteins, or by activating mutations of β-catenin, has been found in several tumour types, and is believed to be a key step in neoplastic progression. Transcriptional targets of the Wnt pathway include the cellular oncogenes cyclin D1 and c-myc. Activation of the Wnt signalling pathway by various means can therefore be a primary cause in oncogenesis, affecting cell proliferation, morphology and contact inhibition, as well as co-operating with other oncogenes in multistep tumour progression.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Higher education 38 (1999), S. 275-290 
    ISSN: 1573-174X
    Keywords: academic staff ; curriculum change ; equity ; governance ; staff development ; student needs ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract In South Africa the restructuring of the higher education system and the transformation of higher education institutions are located within the country's broad political and socio-economic transition to democracy. This paper focuses particularly on institutional transformation, and pays attention to the implications of the process of transformation for academic staff. The following five interlinked and interdependent issues characterizing institutional transformation in South African higher education are identified: democratising the governance structures of institutions increasing access for educationally and financially disadvantaged students restructuring the curriculum focusing on developmental needs in research and community service redressing inequalities in terms of race and gender. Although the overall effect of institutional transformation is experienced rather negatively by many academic staff members, the paper concludes that academics have to be empowered by means of staff development to remain active partners in the transformation process.
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  • 14
    ISSN: 1572-9729
    Keywords: abiotic ; biological ; cell-free extract ; chloroethane ; dechlorination ; 1,1-dichloroethane ; 1,1-dichloroethene ; digester ; methanogenic ; transformation ; 1,1,1-trichloroethane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Anaerobic transformations of 1,1,1-trichloroethane (TCA), 1,1-dichloroethane (DCA), and chloroethane (CA) were studied with sludge from a lab-scale, municipal wastewater sludge digester. TCA was biologically transformed to DCA and CA and further to ethane by reductive dechlorination. TCA was also converted to acetic acid and 1,1-dichloroethene (11DCE) by cell-free extract. 11DCE was further biologically converted to ethene. This pathway was confirmed by transformation tests of TCA, DCA and CA, by tests with cell-free extract, and by chloride release during TCA degradation. With cell-free extract, acetic acid accounted for approximately 90% of the TCA transformed; tests with live cells indicate that the fraction of TCA transformed by this pathway decreased with lower biomass. The dechlorination of DCA to CA and CA to ethane was not stoichiometric. A high rate of TCA removal was observed under the experimental conditions. The results indicate that removal of TCA in anaerobic digestion should be complete, but DCA and CA could persist in a normally operating digester.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    GeoJournal 49 (1999), S. 43-51 
    ISSN: 1572-9893
    Keywords: housing market ; suburbanisation ; transformation ; urban development ; urban renewal ; East Germany
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract The aim of this paper is to analyse the main characteristics of post-socialist urban development in East Germany, especially the differences compared to urban development in other East and Central European countries. In spite of the many similar problems and processes in urban development, specific features of East Germany are characterised by the rapid growth of suburbia, especially in the first phase of transition, by the proceeding activities of urban renewal and revitalisation, and by a lower level of social polarisation and socio-spatial segregation as compared to other post-socialist countries. Important conditions for urban development in East Germany exist in special support programmes, high subsidies and other financial transfers as well as in engaged planning conceptions of the local authorities.
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  • 16
    ISSN: 1573-5028
    Keywords: constitutive expression ; GFP ; GUS ; Musa ; ScBV ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 1369 bp DNA fragment (Sc) was isolated from a full-length clone of sugarcane bacilliform badnavirus (ScBV) and was shown to have promoter activity in transient expression assays using monocot (banana, maize, millet and sorghum) and dicot plant species (tobacco, sunflower, canola and Nicotiana benthamiana). This promoter was also tested for stable expression in transgenic banana and tobacco plants. These experiments showed that this promoter could drive high-level expression of the β-glucuronidase (GUS) reporter gene in most plant cells. The expression level was comparable to the maize ubiquitin promoter in standardised transient assays in maize. In transgenic banana plants the expression levels were variable for different transgenic lines but was generally comparable with the activities of both the maize ubiquitin promoter and the enhanced cauliflower mosaic virus (CaMV) 35S promoter. The Sc promoter appears to express in a near-constitutive manner in transgenic banana and tobacco plants. The promoter from sugarcane bacilliform virus represents a useful tool for the high-level expression of foreign genes in both monocot and dicot transgenic plants that could be used similarly to the CaMV 35S or maize polyubiquitin promoter.
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  • 17
    ISSN: 1573-5028
    Keywords: conditional lethal dominant gene ; Cre/loxP ; Nicotiana tabacum ; site-specific recombinase ; transformation ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic tobacco plants were produced that contained single-copy pART54 T-DNA, with a 35S-uidA gene linked to loxP-flanked kanamycin resistance (nptII) and cytosine deaminase (codA) genes. Retransformation of these plants with pCre1 (containing 35S transcribed cre recombinase and hygromycin (hpt) resistance genes) resulted in excision of the loxP-flanked genes from the genome. Phenotypes of progeny from selfed-retransformed plants confirmed nptII and codA excision and integration of the cre-linked hpt gene. To avoid integration of the hpt gene, and thereby generate plants totally free of marker genes, we attempted to transiently express the cre recombinase. Agrobacterium tumefaciens (pCre1) was cocultivated with leaf discs of two pART54-transformed lines and shoots were regenerated in the absence of hygromycin selection. Nineteen of 773 (0.25%) shoots showed tolerance to 5-fluorocytosine (5-fc) which is converted to the toxic 5-fluorouracil by cytosine deaminase. 5-fc tolerance in six shoots was found to be due to excision of the loxP-flanked region of the pART54 T-DNA. In four of these shoots excision could be attributed to cre expression from integrated pCre1 T-DNA, whereas in two shoots excision appeared to be a consequence of transient cre expression from pCre1 T-DNA molecules which had been transferred to the plant cells but not integrated into the genome. The absence of selectable marker genes was confirmed by the phenotype of the T1 progeny. Therefore, through transient cre expression, marker-free transgenic plants were produced without sexual crossing. This approach could be applicable to the elimination of marker genes from transgenic crops which must be vegetatively propagated to maintain their elite genotype.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 57 (1999), S. 207-210 
    ISSN: 1573-5044
    Keywords: biolistics ; gene expression ; haploid ; transformation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using the PDS-1000/He Biolistic® Particle Delivery System, the microprojectile travel distance, rupture disk pressure and DNA/gold particle concentrations were assessed in order to optimise short and longer-term β-glucuronidase reporter gene expression in microspore-derived embryos of wheat. The effects were also evaluated of using sterile filter paper to support explants and treatment with a high osmoticum medium (0.2 M mannitol/0.2 M sorbitol or 0.4 M maltose). In the optimised procedure, wheat microspore-derived embryos (MDEs), were placed on filter paper and incubated on medium containing 0.4 M maltose, for 4 h pre- and 45 h post-bombardment. Five μl pAHC25 (0.75 mg ml-1 in TE buffer) was precipitated onto 25 μl gold particles (60 mg ml-1 in sterile water), using 20 μl spermidine (0.1 M) and 50 μl CaCl2 (2.5 M). The particles were centrifuged and resuspended in 75 μl absolute ethanol prior to the preparation of 6 macrocarriers. A microprojectile travel distance of 70 mm, a rupture pressure of 1300 p.s.i., and a vacuum of 29′′ Hg were employed. Maltose at 0.4 M in the support medium was the most important factor influencing GUS activity in bombarded tissues. GUS activity, 1 day post-bombardment, reached 52 ± 17 GUS-positive foci/MDE (mean ± s.e.m, n=3), with 17 ± 4 foci/MDE at 15 days, giving a 3.0-fold increase (p〈0.05) compared to expression in MDEs bombarded on medium without a high osmoticum treatment.
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  • 19
    ISSN: 1573-5044
    Keywords: ß-glucuronidase ; dendrobium ; hygromycin phosphotransferase ; orchid ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protocorms of orchid (Dendrobium hybrid) were transformed by microprojectile bombardment with a helium-pressured PDS 1000 particle gun. Gold particles coated with plasmid DNA containing ß-glucuronidase (GUS) and hygromycin phosphotransferase (Hpt) marker genes were used. Potentially transformed tissues were identified by active growth on MS medium supplemented with 50mg l-1 hygromycin. After 4–6 months of continuous selection, 15 hygromycin-resistant lines were recovered. Integration of transgenes into the genome of the transformed protocorms and plantlets were confirmed by GUS histochemical assay and Southern blot hybridization. The transgenic protocorms have gone through propagation for more than 8 months and maintained their transgenic characters. These results indicate that we have established a system for orchid transformation in a relatively high frequency and the transgenes are stably expressed in the transgenic plants.
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  • 20
    ISSN: 1573-5079
    Keywords: Chlamydomonas reinhardtii ; LHC II phosphorylation ; mutagenesis ; Photosystem II redox control ; state 2 to state 1 transition ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oxygenic photosynthetic organisms adapt to varying light conditions by changing the distribution of light energy between Photosystem II (PS II) and photosystem I (PS I) during so-called state transitions. To identify the genes involved in this process, we have exploited a simple chlorophyll fluorescence video-imaging technique to screen a library of nuclear mutants of Chlamydomonas reinhardtii for colonies grown on agar plates that are disturbed in their ability to regulate light energy distribution between PS I and PS II. Subsequent modulated fluorescence measurements at room temperature and 77 K fluorescence emission spectra confirmed that 5 mutants (0.025% of total number screened) were defective in state transitions. [32P]orthophosphate phosphorylation experiments in vivo revealed that in one of these mutants, designated stm1, the level of LHC II polypeptide phosphorylation was drastically reduced compared with wild type. Despite WT levels of PS I and PS II, stm1 grew photoautotrophically at reduced rates, compared with WT especially under low light conditions, which is consistent with an important physiological role for state transitions. Our results highlight the feasibility of video imaging in tandem with mutagenesis as a means of identifying the genes involved in controlling state transitions in eukaryotic photosynthetic organisms.
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  • 21
    ISSN: 1573-6784
    Keywords: Bacillus ; plasmids ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple and easy method for the introduction of plasmid DNA into different species of Bacillus was developed. The method involves the suspension in a transformation buffer of nutrient agar grown cells in their late exponential phase and the addition of unpurified plasmid DNA. Transformants were obtained at a frequency of about 103 to 105 stable transformants per μg of plasmid DNA.
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  • 22
    ISSN: 1573-2932
    Keywords: arid-zone soils ; field capacity ; fractionation ; heavy metals ; kinetics ; redistribution ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Solid-phase transformation of added Cd, Cu, Cr, Ni, Pb and Zn, in two arid-zone soils incubated in the field capacity moisture regime for one year, were studied. The heavy metals were fractionated into six empirically defined fractions using a selective sequential dissolution (SSD) protocol optimized for arid-zone soils. Each of these fractions was named based on the major soil component targeted for dissolution during the specific SSD step, but it is not assumed that they are mineralogically and chemically totally specific. The transformations of the metals in the two soils incubated at the field capacity regime were compared with those at the moisture saturation regime (Han and Banin, 1997). An initial fast stage of transformation of the soluble metals from the exchangeable (EXC) fraction to the less labile fractions (the carbonate (CARB) fraction for Cd, Pb, Zn, Ni and Cu, and the organic matter (OM) fraction for Cr, and to some extent Cu and Ni) occurred during the fractionation and within one hour after addition. This was followed by a second stage, involving long-term transformation processes of all metals: added Cd was transferred from the EXC into the CARB fraction; added Cr was transferred from the CARB to the OM fraction and Pb was transferred very slowly to the easily reducible oxide (ERO) fraction. Added Cu, Ni and Zn were transferred from the EXC and CARB fractions into the ERO fraction and to some extent OM and RO fractions. In Part I of this series, we reported that during incubation in the saturated moisture regime, Zn and Ni were transferred mainly into the RO and OM fractions. Cadmium, Cr and Pb underwent the same transformation pathways during the slow long-term process, with slightly different rates, in both water regimes. At low levels of addition, the incubated soils moved over one year towards a distribution similar to that of the native soil. At higher levels, the soils still remained removed from the quasi-equilibrium which characterized the native soil, even at the end of one year of incubation.
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    Chemie in unserer Zeit 33 (1999) 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Chemie in unserer Zeit 33 (1999), S. 45-50 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A variety of demonstration experiments can be shown to a large audience with a minimum amount of chemicals by using an ordinary slide projector and easily mounted cuvettes. A detailed instruction for assembling the cuvettes is given as m d l as examples for suitable demonstration experiments in general, inorganic, organic and physical chemistry.
    Additional Material: 21 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 61-61 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Chemie in unserer Zeit 33 (1999), S. 67-67 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 28
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    Chemie in unserer Zeit 33 (1999) 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 29
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    Chemie in unserer Zeit 33 (1999), S. 95-103 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Natural events as well as human activities are affecting the environment and even with largescale monitoring programs it is not possible to completely assess the state of the environment. But a documentation of the environmental status with respect to its chemical composition can be realized with systematically collected representative environmental samples, which can be stored for decades at very low temperatures almost without degradation. Such ‘banking activity’ allows a current environmental monitoring and also a retrospective analysis for the determination of components which have not been analyzed at the time of sampling because of ta lack in analytical techniques or because they have not been known or considered environmentally relevant. The concept of environmental specimen banking and general procedures are described. Various examples illustrate the many facets of such activities.
    Additional Material: 13 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 128-128 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die Arbeitsmarktsituation in der Chemie hat sich in den letzten Jahren spürbar entspannt. Dennoch gibt es auf etlichen, nicht hundertprozentig dem Trend der Zeit entsprechenden Spezialgebieten immer noch einen beträchtlichen Überhang an Stellensuchenden. Entsprechende Mobilität vorausgesetzt, kann das Internet bei der Stellensuche hilfreich sein.
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    Chemie in unserer Zeit 33 (1999), S. 135-135 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 32
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    Chemie in unserer Zeit 33 (1999) 
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    Keywords: Chemistry ; Chemistry
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  • 33
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    Chemie in unserer Zeit 33 (1999), S. 165-166 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 34
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    Chemie in unserer Zeit 33 (1999), S. 158-164 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Atmospheric aerosol particles cause one of the largest uncertainties in estimates of human influence on climate - a good reason to take a closer look at the atmospheric life cycle of the aerosols and its effects. Besides a number of primary and secondary natural aerosol sources we need to consider since the beginning of industrialisation strong manmade particle sources. During its residence in the atmosphere the aerosol interacts in many physical and chemical ways with other atmospheric trace substances, most importantly with water vapor and its liquid and solid phases. Through its direct effect on solar and thermal radiation and through its influence on clouds the atmospheric aerosol exerts a climate forcing. To data we cannot predict the ensuing climate response because of our limited understanding of essential atmospheric processes and of the many possible feedbacks within the climate system. However, already our present knowledge of the role of the atmospheric aerosol in the climate system makes strictly global views of anthropogenic climate changes questionable.
    Notes: Der Stand unseres Wissens zum Kreislauf des Atmosphärischen Aerosols und von seiner Rolle im Klimasystem läßt sich wie folgt zusammenfassen: 1Unser Verständnis des globalen Aerosolkreislaufs und vor allem des menschlichen Eingriffs in denselben ist noch sehr unvollständig. Dies gilt in hohem Maße auch für die mit diesem Kreislauf verbundenen chemischen Mehrphasenprozesse.2Ein negativer direkter Klimaantrieb anthropogener Aerosole existiert über die Streuung und Absorption, hat aber noch große Unsicherheiten und kann über hoch mit Rußpartikeln belasteten Gebieten auch positive Werte annehmen.3Ein indirekter Klimaantrieb anthropogener Aerosole existiert wahrscheinlich und hat ebenfalls ein negatives Vorzeichen. Seine Größenordnung und Unsicherheiten lassen sich noch nicht quantifizieren.
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    Chemie in unserer Zeit 33 (1999), S. 177-185 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Chemical concepts are important to gain a qualitative understanding of chemistry. They are needed to find interesting questions to which experimental research as well as quantum-chemical investigations and computer simulations can contribute. We point out why chemical concepts should be seen as an important and from fundamental physical theories to a certain degree independent part of chemistry. An overview and analysis of some concepts - which are often introduced only in connection with special examples instead of a discussion for their own sake - are given. We take a closer look at some concepts to illustrate our assertions. An analysis of the stability of the cyclic (MoNL3)-trimer demonstrates how these concepts work and what they can tell us about this special case.
    Notes: Wir haben versucht, deutlich zu machen, wie wichtig theoretische Konzepte in der Chemie sind. Dabei gelangen wir zu folgenden Schlußfolgerungen über die Konzepte, die in der Chemie herangezogen werden, um Beobachtungen zu deuten und zu verstehen: Qualitativ chemische Konzepte sind für ein Verständnis der Chemie unverzichtbar. Eine quantenchemische Berechnung liefert nicht automatisch Verständnis und Einordnung in einen Gesamtzusammenhang.Erst die vereinfachende Beschreibung chemischer Konzepte ermöglicht eine unmittelbare Diskussion chemischer Fragestellungen.Die Konzepte sind trotz grundlegender physikalischer Theorien bis zu einem gewissen Grade eigenständig.
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  • 36
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    Chemie in unserer Zeit 33 (1999) 
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    Chemie in unserer Zeit 33 (1999), S. 213-220 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The article commemorates the centenary of the protection of the proprietary mine of Aspirin. The early medicinal uses of salicylic acid derivatives in herbal remedies arc summarised as well as the biosynthesis of these derivatives. The history of the synthesis of salicylic acid and of acetyl salicylic acid (ASS) is described in detail as is the discovery of additional therapeutic applications and the basic pharmacology of ASS.
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    Chemie in unserer Zeit 33 (1999), S. 234-235 
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    Chemie in unserer Zeit 33 (1999), S. 246-246 
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    Chemie in unserer Zeit 33 (1999), S. 250-250 
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    Chemie in unserer Zeit 33 (1999), S. 308-309 
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    Chemie in unserer Zeit 33 (1999), S. 312-312 
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    Chemie in unserer Zeit 33 (1999), S. 314-314 
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  • 45
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    Chemie in unserer Zeit 33 (1999), S. IX 
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    Chemie in unserer Zeit 33 (1999), S. 318-318 
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  • 47
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    Chemie in unserer Zeit 33 (1999), S. 354-358 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Chemical Biology is a rapidly growing field at the interface of organic chemistry and cell biology. Chemical strategies are used to address biological questions not amenable to traditional genetic and biochemical techniques alone. Thc enormous potential of Chemical Biology is illustrated by four recent examples.
    Notes: Chemische Biologie ist eine junge Forschungsrichtung im Grenzgebiet zwischen Organischer Cherme und Zellbiologie, welche aus einem Bedarf nach neuen Techniken zur Untersuchung zellbiologischer Mechanismen entstanden ist. Chemische Methoden werden mit biochemischen und molekularbiologischen Methoden kombiniert, um neuartige Möglichkeiten für die biologische Grundlagenforschung zu schaffen. So werden synthetische Oligosaccharide kovalent an die Oberfläche lebender Zellen gebunden, die Konzentration von Calciumionen im Zytosol künstlich reguliert und Rezeptoren künstlich aktiviert.
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    Chemie in unserer Zeit 33 (1999), S. 373-374 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Eine neue Elektrosynthese erfüllt erstmals den Traum der Elektrochemiker, gleichzeitig Anoden- und Kathodenreaktion für organische Synthesen technisch zu nutzen.
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    Chemie in unserer Zeit 33 (1999), S. 377-377 
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    Chemie in unserer Zeit 33 (1999), S. 380-380 
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    Chemie in unserer Zeit 33 (1999), S. 382-384 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Around the world, BASF has been preparing for Y2K since 1996. The Information services and the Process Control Technology divisions, as well as embedded systems and logistics, not to mention the company's customers and suppliers have all been systenmatically tested for Y2K capability. In addition, special precautionary measures in particular, at large Verbund sites - will be in place during the switchover period.
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    Chemie in unserer Zeit 33 (1999), S. 4-4 
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    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    Chemie in unserer Zeit 33 (1999), S. 104-109 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The Fonds of German Chemical Industry (FCI) has produced an instrumental kit named „Blue Genes“ which enables High School teachers to perform in their school environment basic experiments of gene analysis and cloning. In particular, the kit contains all necessary equipment and reagents to perform a restriction analysis, and cloning and expression of a bacterial gene.
    Additional Material: 7 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 152-157 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: A few physical phenomena believed to be religious miracles are considered, and scientific explanations for them are proposed.
    Notes: Einige physikalische Phänomene, von denen man glaubte, sie seien religiöse Wunder, werden vorgestellt und wissenschaftliche Erklärungen hierfür vorgeschlagen.
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    Chemie in unserer Zeit 33 (1999), S. 173-173 
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    Chemie in unserer Zeit 33 (1999), S. VIII 
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    Chemie in unserer Zeit 33 (1999), S. XIV 
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    Chemie in unserer Zeit 33 (1999), S. 334-341 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The essential trace element copper plays a key role in living systems. Its metalloproteins are involved in various reactions. The IT ononuclear blue copper type-1 proteins take, part in electron transfer processes. Type-2 proteins can either act as oxidases, oxygenases, dioxygenases, or superoxide dismutases, containing one copper core. Dinuclear copper centers of Type-3 proteins are the oxygen transport system hemocyanin, oxidases and oxygenases. Due to this variety of functions copper deficiency and also abundance can cause severe damage of any life form.
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    Chemie in unserer Zeit 33 (1999), S. 359-364 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: It is tried to fix the limits between the notions high pressure synthesis and high temperature chemistry. The work of Fritz Haber to apply high pressure to synthetise ammonia and of Friedrich Bergius to liquify coal to synthetise petrol is described. Special attention is given to the merits of Carl Bosch and his coworkers at BASF for applying the high pressure synthesis into industrial scale. The consequences of thc work of Haber, Bergius und Bosch on chemistry, social and political life and their importance for the present are considered.
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    Chemie in unserer Zeit 33 (1999), S. 376-376 
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    Chemie in unserer Zeit 33 (1999), S. 390-390 
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    Chemie in unserer Zeit 33 (1999), S. 6-19 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Diatoms are unicellular organisms which possess a unique silicified cell wall. Their intricate structures have inspired the imagination of naturalists and scientists throughout centuries. However, molecular biological details about silica metabolic pathways still are obscure. To find out how soft matter can selforganize and create finite solid shapes and complex patterns is certainly one of the most challenging scientific problems today. Especially chemists just have started to unravel the mysteries of chemical morphogenesis.
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    Chemie in unserer Zeit 33 (1999), S. 58-60 
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    Chemie in unserer Zeit 33 (1999), S. 66-66 
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    Chemie in unserer Zeit 33 (1999), S. 70-70 
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    Chemie in unserer Zeit 33 (1999), S. 122-125 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    Chemie in unserer Zeit 33 (1999), S. X 
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    Chemie in unserer Zeit 33 (1999), S. 138-138 
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    Chemie in unserer Zeit 33 (1999), S. 174-175 
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    Chemie in unserer Zeit 33 (1999), S. 190-190 
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    Keywords: Chemistry ; Chemistry
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    Chemie in unserer Zeit 33 (1999), S. 342-353 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Cyclopentadienyl (Cp) complexes of maingroup elements are classified and compared in terms of structural types, bonding features, and characteristic reactions. Halfsandwich, sandwich and multidecker sandwich arrangements of different type are observed including cluster species with weak or strong metal-metal bonds. The Cp-El bonding ranges from mainly covalent to purely ionic with important structure-determining intermolecular contacts in the latter. π-Complexation allows the stabilisation of compounds with low-valent elements such as AI(I), Ga(I), and Si(I1). Important reaction principles and applications are shortly described.
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    Chemie in unserer Zeit 33 (1999), S. 367-372 
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    Chemie in unserer Zeit 33 (1999), S. 375-375 
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    Chemie in unserer Zeit 33 (1999), S. 381-381 
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    Chemie in unserer Zeit 33 (1999), S. 390-390 
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    Chemie in unserer Zeit 33 (1999), S. 53-58 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 2 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 62-63 
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    Chemie in unserer Zeit 33 (1999), S. 129-129 
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    Chemie in unserer Zeit 33 (1999), S. 134-134 
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    Chemie in unserer Zeit 33 (1999), S. V 
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    Chemie in unserer Zeit 33 (1999), S. VIII 
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    Chemie in unserer Zeit 33 (1999), S. 186-186 
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    Chemie in unserer Zeit 33 (1999), S. 192-198 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 13 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 221-225 
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In the last decade, major advances in the understanding of the biology of aging were made. Studies in worms, flies and yeast led to the identification of several life-extending genes. In mammals, two genes were found which, when mutated, shorten life span. Previous studies in rodents reporting life span prolongation by caloric restriction were confirmed and extended to lower organisms. The studies arc consistent with and supportive of the free radical theory of aging, which secs reactive oxygen species as the main contributor to aging. A refinement of this theory puts mitochondrial DNA into the center of the aging process. It appears that mitochondrial DNA may be both, a yardstick for the biological age and a signalling device to tell the organism when to die. A diet low in calories and high in antioxidants promises to lead to a better quality of life at old age and possibly to increased life span for humans.
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    Chemie in unserer Zeit 33 (1999), S. 237-239 
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    Chemie in unserer Zeit 33 (1999), S. 241-241 
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    Chemie in unserer Zeit 33 (1999), S. 247-247 
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    Chemie in unserer Zeit 33 (1999), S. 252-266 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This first part of „Rechargeable Batteries“ is devoted to materials and reactions in batteries with aqueous electrolytes. Lead-acid, nickelcadmium, nickel-metal hydride, and various zinc systems, such as zinc-manganese oxide, zinc-air, and zinc-bromine cells are discussed. Special attention is paid to the limited electrochemical stability window of the aqueous electrolytes, which enables overcharge and overdischarge protection of the battery but limits cell voltages to less than 2 Volts. In the following part of this article we will focus on nonaqueous electrolyte-based rechargeable batteries. Due to thc wider electrochemical stability window of these electrolytes high specific charge and high cell voltage batteries can be realized, e.g., the „4 V“ lithium ion cell.
    Additional Material: 19 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 304-308 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 2 Ill.
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    Chemie in unserer Zeit 33 (1999), S. 314-314 
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    Chemie in unserer Zeit 33 (1999), S. 390-390 
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    Chemie in unserer Zeit 33 (1999), S. 33-44 
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    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Solid solutions arc an area of inorganic chemistry that encompasses a wide variety of natural and synthetic compounds. This article is intended to be a selection of interesting examples which illustrate the importance of solid solutions in everyday life and industrial applications. A short discussion of the theoretical background is given, followed by the description of solid solutions of non-metals in metals such as steel. Furthermore, solid solutions with important optical (e.g. laser materials) and electrical properties (e.g. β-alumina) are discussed, referring to their chemical structure and technological relevance.
    Additional Material: 13 Ill.
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