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  • Digitale Medien  (4)
  • 1980-1984  (4)
  • Recombinant DNA
  • Industrial Chemistry
  • Nuclear reactions
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Materialart
  • Digitale Medien  (4)
Erscheinungszeitraum
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  • 1
    Digitale Medien
    Digitale Medien
    Cloning of the ribokinase gene of Staphylococcus hyicus subsp. hyicus in Staphylococcus carnosus (1984)
    Springer
    Archives of microbiology 140 (1984), S. 218-224 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Recombinant DNA ; Cloning ; Staphylococcus carnosus and S. hyicus subsp. hyicus ; Ribose degradation ; Ribokinase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A gene library with DNA of Staphylococcus hyicus subsp. hyicus was established in S. carnosus by using the plasmid vector pCT20. Two clones of S. carnosus were isolated which were able to ferment d-ribose. The two hybrid plasmids (pRib 1) and (pRib 2) were isolated and characterized. They contained inserted DNA fragments of S. hyicus subsp. hyicus with sizes of 10.2 and 8.2 kb, respectively. d-Ribose uptake and enzyme activities were studied. All strains tested [S. hyicus subsp. hyicus, S. carnosus (wild type) and the two S. carnosus clones] possessed an inducible uptake system for d-ribose. S. hyicus subsp. hyicus possessed in addition enzyme activities of d-ribokinase and d-ribose-5-P isomerase. None of these enzyme activities could be detected in S. carnosus (wildtype). Only in the S. carnosus clones containing (pRib 1) or (pRib 2) could a d-ribokinase activity be demonstrated, indicating that the gene for d-ribokinase of S. hyicus subsp. hyicus was cloned in S. carnosus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00454931
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  • 2
    Digitale Medien
    Digitale Medien
    Cloning and analysis of recombinant plasmids containing genes for Aspergillus nidulans 5 S rRNA (1981)
    Springer
    Current genetics 4 (1981), S. 173-176 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus ; 5S rRNA ; Recombinant DNA ; Restriction Analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genes coding for 5S rRNA were found to be dispersed in the Aspergillus nidulans genome. Three different recombinant plasmids hybridizing to 5S rRNA were isolated and their restriction enzyme maps were established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420494
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  • 3
    Digitale Medien
    Digitale Medien
    Cloned ß-galactosidase gene of Escherichia coli is expressed in the yeast Saccharomyces cerevisiae (1980)
    Springer
    Current genetics 2 (1980), S. 109-113 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Recombinant DNA ; 2 µm DNA ; Heterologous gene expression ; Eukaryotic host
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The expression of the lacZ gene of E. coli in S. cerevisiae has been studied. The enzymatic activity coded by the lacZ gene in E. coli, β-galactosidase, is detectable in yeast cells harboring a chimeric plasmid carrying the gene. On the basis of size and immunological criteria, no difference was detected between the Coli-in-yeast β-galactosidase and the E. coli enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420622
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  • 4
    Digitale Medien
    Digitale Medien
    Use of an (n, α) nuclear reaction to study the long-distance transport of boron in trifolium repens after foliar application (1980)
    Springer
    Planta 150 (1980), S. 197-205 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Boron ; Foliar nutrition ; Nuclear reactions ; Transport (boron) ; Trifolium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The growth of white clover (Trifolium repens L.) is severely inhibited by boron starvation, but a foliar treatment with boric acid can transitorily alleviate the deficiency symptoms. The 10B(n ,α)7 Li nuclear reaction has been used to study boron transport in the plant after foliar application. More than 98% of the boron supplied remained at the point where it was applied to the leaves, and less than 2% was useful to the growth of the treated plant. This small “efficient” portion of boron was quite mobile. It was distributed to the different parts of the plant, then was transferred from the oldest parts to the newly formed leaves. Physiological and agronomical implications of these data are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00390826
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