ISSN:
0269-3879
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The thin-layer chromatographic (TLC) behaviour of liposomes containig inositol phosphates (IPs) was studied. The liposomes contained different concentrations of D-myo-inositol 1, 4, 5-trisphosphate (IP3), D-myo-inositol 1, 2, 6-trisphosphate (α-trinositol, PP 56, a novel Perstorp Pharma derivative), D-myo-inositol 1, 3, 4, 5-tetrakisphosphate (IP4), D-myo-inositol 1, 3, 4, 5, 6-pentakisphosphate (IP5) and D-myo-inositol 1, 2, 3, 4, 5, 6-hexakisphosphate (IP6). Migration of all liposome batches was compared to that of control liposomes (containing only triple-distilled water), and to that of free phosphatidylcholine (PC); the same amount of lipid was used in all situations.Thin-layer chromatography was performed on silica gel as adsorbent. As solvent we used an n-buthanol: ethanol: water mixture in a 4:3:3 volume ratio. Significant differences were found between PC and all liposome batches, as well as between control liposomes and the ones containing IP3, α-trinositol, IP4, or IP5, in various concentrations. Liposomes containing IP6 migrate completely differently compared not only to phosphatidylcholine and control liposomes, but also to the ones containing other IPs (〈10-3 M). Unlike the other IPs studied, liposome-entrapped IP6 elicits dose-independent contractions of the isolated rat aorta. This suggests that liposomes loaded with IP6 undergo, during or after their preparation, physico-chemical alterations that eventually change their drug-delivery capacity.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bmc.1130090405
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