ISSN:
1573-5028
Keywords:
cDNA
;
Phytolacca anti-viral protein
;
ribosome-inactivating proteins
;
sequencing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00037047
