ISSN:
1617-4623
Keywords:
Mutagen hyper-resistance
;
4-nitroquinolineN-oxide
;
Yeast
;
ATP-dependent permease
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The yeast gene SNQ2 confers hyper-resistance to the mutagens 4-nitroquinoline-N-oxide (4-NQO) and Triaziquone, as well as to the chemicals sulphomethuron methyl and phenanthroline when present in multiple copies in transformants of Saccharomyces cerevisiae. Subcloning and sequencing of a 5.5 kb yeast DNA fragment revealed that SNQ2 has an open reading frame of 4.5 kb. The putative encoded polypeptide of 1501 amino acids has a predicted molecular weight of 169 kDa and has several hydrophobic regions. Northern analysis showed a transcript of 5.5 kb. Haploid cells with a disrupted SNQ2 reading frame are viable. The SNQ2-encoded protein has domains believed to be involved in ATP binding and is likely to be membrane associated. It most probably serves as an ATP-dependent permease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00277115
