ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Negative electrospray ionization mass spectrometry (NESI-MS) of a series of native gangliosides containing one, two or three sialic acid groups, sulphatide-I and cholesterol 3-sulphate was undertaken, using a magnetic sector instrument. The results show that NESI-MS of gangliosides leads to the formation of multiple-charged molecular ions of the type [M — H]-, [M — 2H]2- and [M — 3H]3-. The number of charges appears to be correlated to the number of sialic acid groups present. NESI-MS of sulphatide-I predominantly gave ions of type [M — H]- whilst cholesterol 3-sulphate gave a more complex ionization pattern. The sensitivity of the NESI-MS system was enhanced by using water+methanol, 9:1 (v/v) as solvent, containing 300 pmol/μL of NH4HCO3. The application of NESI-MS to these compounds is discussed.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290090604
