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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of Forssman and Other Antigen/Antibody Systems in Vascularized Mouse Heart to Rat Xenotransplantation (2001)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 53 (2001), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study, the nature of hyperacute xenograft rejection was closely studied in a vascularized mouse-to-rat transplantation model. Antibodies against mouse heart, erythrocytes and lymphocytes and against the Forssman antigen were raised in the rat. Upon heterotopic heart transplantation the respective antisera were intravenously (i.v.) injected. Passive transfer of antiheart, antierythrocyte or antilymphocyte serum resulted in hyperacute rejection of the transplanted mouse heart. Subfractionation of the antiheart serum showed that the capacity to induce hyperacute rejection was carried by the immunoglobulin (Ig)G fraction. When antierythrocyte serum adsorbed with mouse erythrocytes was administered the cardiac grafts remained beating. To the contrary, antilymphocyte serum adsorbed with erythrocytes still had the capacity to induce hyperacute rejection. None of the rats that had previously been challenged with the Forssman antigen rejected their grafts hyperacutely. Subsequent investigations by electron microscopy revealed that the Forssman antigen is expressed on dendritic cells (DC) adjacent to the vessels, but not on the vascular endothelium, thus explaining the inability of the anti-Forssman serum to induce hyperacute rejection. Taken together, we have demonstrated the existence of several xenoantigens that can be targets for antibody-mediated rejection, suggesting that more than one relevant xenoantigen exists also in more distantly related combinations, such as the pig-to-human combination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2001.00840.x
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  • 2
    Electronic Resource
    Electronic Resource
    Decreasing Ratios of Phosphocreatine to β-ATP Correlates to Progressive Acute Rejection in a Concordant Mouse Heart to Rat Xenotransplantation Model (2001)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 53 (2001), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Biopsies are difficult to perform in rodent heart transplant models without compromising the graft function and therefore other means to evaluate the grafts repeatedly and noninvasively are warranted. The goal of the present study was to measure changes in ratios of high energy phosphorus containing metabolites detected with in vivo31Phosphorous Magnetic Resonance Spectroscopy (31P MRS) in a xenotransplantation model and to investigate if these ratios correlated to histological signs of acute xenograft rejection. Thirty-five heart transplantations were performed (NMRI-mice to Lewis (RT11) rats). Thirteen heart transplants underwent repeated daily in vivo31P MRS measurements and 22 grafts were measured on any of 4 postoperative days and thereafter sacrificed for histology. A modified scoring system based on Billingham's criteria was used to stage the rejection process. The median graft survival was 3.0 ± 0.44 (median ± SD) days (n = 17). Significant differences, both overall and interday, could be calculated for the phosphocreatine (PCr)/β-adenosine triphosphate (β-ATP) ratios and for the rejection score. The decreases in PCr/β-ATP ratios correlated significantly to the progressive acute rejection process in the sacrificed grafts (P = 0.01). Further studies are indicated to establish the potential of 31P MRS in immunosuppressed recipients of vascularized xenotransplants with prolonged graft survival.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2001.00849.x
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  • 3
    Electronic Resource
    Electronic Resource
    Negative electrospray ionization mass spectrometry analysis of gangliosides, sulphatides and cholesterol 3-sulphate (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 491-494 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Negative electrospray ionization mass spectrometry (NESI-MS) of a series of native gangliosides containing one, two or three sialic acid groups, sulphatide-I and cholesterol 3-sulphate was undertaken, using a magnetic sector instrument. The results show that NESI-MS of gangliosides leads to the formation of multiple-charged molecular ions of the type [M — H]-, [M — 2H]2- and [M — 3H]3-. The number of charges appears to be correlated to the number of sialic acid groups present. NESI-MS of sulphatide-I predominantly gave ions of type [M — H]- whilst cholesterol 3-sulphate gave a more complex ionization pattern. The sensitivity of the NESI-MS system was enhanced by using water+methanol, 9:1 (v/v) as solvent, containing 300 pmol/μL of NH4HCO3. The application of NESI-MS to these compounds is discussed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290090604
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  • 4
    Electronic Resource
    Electronic Resource
    S12.7 Carbohydrate antigens as targets for xenoreactive antibodies in a mouse to rat transplantation model (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 297-297 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01210041
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