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Digitale Medien

Chemiluminescence high performance liquid chromatography of corticosteroids using lucigenin as post-column reagent (1990)

Takeda, Motoko ; Maeda, Masako ; Tsuji, Akio

New York, NY : Wiley-Blackwell

Biomedical Chromatography 4 (1990), S. 119-122

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Details

ISSN: 0269-3879

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: A chemiluminescence high performance liquid chromatographic method for the determination of corticosteroids and tetrahydrocorticosteroids has been developed. Corticosteroids and their metabolites extracted from urine samples were separated using an ODS column and a mixture of methanol + water + 0.01 M sodium acetate solution (70:30:5) as eluent. The eluent from the column was mixed with the chemiluminescent solution containing lucigenin and Triton X-100 and a 0.28 M KOH solution by pumps and monitored by a chemiluminescence detector. No interference was encountered and the method is both precise and reproducible.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bmc.1130040309

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