ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Mass spectrometric determination of moth pheromone components by means of selected ion monitoring with electron impact and chemical ionization has been evaluated. For the analysis of acetates, chemical ionization with ammonia as reactant gas proved advantageous with respect to sensitivity and selectivity. A quantitative assay for determination of pheromone samples from individual turnip moth females (Agrotis segetum, Noctuidae), using internal standards, deuterated at the acyl group, was developed. The assay showed correct linearity in the investigated region (20-2000 pg). Signal to noise ratios for 100 pg of acetates using chemical ionization (ammonia as reactant gas) were in the range 10-50 depending on the degree of unsaturation. Conservative limits of detection (background signal + three times the standard deviation) and quantification (background signal + 10 times the standard deviation) were approximately 70 and 240 pg, respectively. A significant decrease in gland titre of (Z)-5-decenyl acetate and (Z)-7-dodecenyl acetate after mating was established.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200110303
