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Maintaining perpetual synchrony in HeLa S3 culture (1974)

Thilly, W. G. ; Nowak, T. S. ; Wogan, G. N.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 16 (1974), S. 149-156

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: HeLa S3 cells in suspension culture are resynchronized in each successive generation by exposure to 0.25mM thymidine. The resynchronization approach appears to be superior to single- and double-block techniques because the period of effective inhibition of DNA synthesis is significantly shortened. Gram quantities of highly synchronous viable cells have been produced on a daily basis.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260160111

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