ISSN:
0006-3592
Keywords:
Monte Carlo simulation
;
depolymerization
;
endo-enzymes
;
single-attack mechanism
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A Monte Carlo simulation of the depolymerization of linear homopolymers by specific endo-enzymes exhibiting random-attack probability and a single-attack mechanism has been developed. The program simulates the “real” depolymerization versus time of a polydisperse sample of substrate by a specific endo-enzyme. Given the initial mass distribution and concentration of the substrate, the initial concentration of the enzyme, and its Michaelis-Menten constant, the program simulates the evolution of the mass distribution of the substrate with the depolymerization time. When tested against experimental data from the depolymerization of barley (1→3),(1→4)-β-D-glucan by malt endo-(1→3),(1→4)-β-D-glucanase, monitored using the Calcofluor-FIA method with fluorescent detection, excellent results were obtained. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 105-113, 1998.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
