ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Protein production capabilities ofTrichpolusia ni (TN 368) cells andSpodoptera frugiperda (Sf9) cells were compared in GTC100 medium in suspension culture using as a vector a genetically engineeredAutographa californica nuclear polyhedrosis virus. TN 368 produces more β-galactosidase than Sf9, on a per cell basis (2.2×105 and 1.7×105 units/ 106 cells1 respectively). In growth experiments serum-free medium supported a higher maximum Sf9 cell density (4±1×106 cells/ml) than the serum- based media (1.5±5×106 cells/ml in GTC100 and 2±1×106 cells/ml in TNM-FH). However, using a cell density of 5×05 cells/ml, the productivity per cell varied, from a low of 4.5×104 units in EX-CELL-400 medium to a high of 7.6×104 units in TNM-FH. The TN 368 cells were twice a large as Sf9 cells and appeared to be more shear sensitive than Sf9 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01041482
