ISSN:
1573-5028
Schlagwort(e):
cis-regulatory elements
;
Vicia faba
;
seed protein gene
;
promoter deletions
;
seed-specific expression
;
transgenic tobacco
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract We have identified cis-regulatory elements within the 5′-upstream region of a Vicia faba non-storage seed protein gene, called usp, by studying the expression of usp-promoter deletion fragments fused to reporter genes in transgenic tobacco seeds. 0.4 kb of usp upstream sequence contain at least six, but probably more, distinct cis-regulatory elements which are responsible for seemingly all quantitative, spatial and temporal aspects of expression. Expression-increasing and-decreasing elements are interspersed and include an AT-rich sequence, a G-box element and a CATGCATG motif. The latter acts as a negative element in contrast to what has been found for the same motif in legumin-and vicilin-type seed storage protein gene promoters. Seed specificity of expression is mainly determined by the −68/+51 region which confers, however, only very low levels of expression. The data support the combinatiorial model of promoter function.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00047407
