ISSN:
1432-1440
Keywords:
Platelet-associated IgG
;
Micro-enzyme-linked immunoassay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An enzyme linked immunoassay was developed using the microtiter system to measure platelet-associated IgG (PAIgG). One single determination needs 2×106 platelets usually obtained from 3 ml blood anticoagulated with EDTA. Platelets are incubated with peroxidase-conjugated anti-human IgG. Its unbound fraction is adsorbed to an IgG coated microtiter plate, quantitated by a colour reaction and found to be inversely related to the amount of PAIgG. Healthy donors (n=40, aged 1 day to 35 years) possessed 3.6±2.0 (mean±S.D.) fg IgG/platelet. Increased levels of PAIgG were found in patients with acute (n=16) and chronic (n=5) idiopathic thrombocytopenic purpura. Postrecovery platelets had normal values of PAIgG. Widely varying levels of PAIgG were found in patients with freshly diagnosed acute lymphocytic leukemia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01486372
