ISSN:
1432-072X
Keywords:
Clostridium histolyticum
;
Glycine
;
Arginine
;
Threonine
;
Selenium
;
Glycine reductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Clostridium histolyticum grew on glycine, arginine, or threonine as sole substrate. Arginine degradation preceded that of glycine and partially inhibited that of threonine when two amino acids were present. Each amino acid seemed to be individually catabolized, not by a Stickland type of reaction. Glycine fermentation required the presence of complex ingredients. Therefore, an effect of selenite on glycine catabolism could only be demonstrated after scavenging selenium contamination by preculturing Peptostreptococcus glycinophilus in that medium. C. acidiurici was not suited as selenium accumulating organism as C. histolyticum was inhibited by the residual uric acid. Arginine catabolism was unaffected by seleniuum depriviation. The labelling pattern obtained in acetate after incubation of C. histolyticum with [1-14C]- or [2-14C]glycine strongly indicated the metabolism of glycine via the glycine reductase pathway.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409710
