ISSN:
1432-072X
Keywords:
Lipopolysaccharide
;
Sialic acids
;
Core region
;
Purple nonsulfur bacteria
;
N-Acetylneuraminic acid
;
Rhodobacter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Lipopolysaccharides (LPS) from a number of purple nonsulfur bacteria and of phylogenetically related species were analyzed for the presence of sialic acid by gas chromatography/mass spectrometry. Species and strains of the genera Rhodobacter, Rhodopseudomonas, Rhodomicrobium, Rhodospirillum, Rhodocyclus and Rhodopila were investigated, sialic acid, however, was found only in the genus Rhodobacter. It occurs in strains of Rhodobacter capsulatus, R. sphaeroides, R. sulfidophilus and R. veldkampii. All these species belong to the α-3 subgroup of purple bacteria as defined by 16S rRNA catalogues. Approximately equimolar ratios of sialic acid and of 2-keto-3-deoxy-octonate (KDO) were found in isolated LPSs. Sodium deoxycholate gel electrophoresis of these LPS-samples also suggested a location of sialic acid in the LPS “core” region. Sialic acid was present only in those LPSs, which exhibited a “complete core region”.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00408254
