ISSN:
1432-072X
Keywords:
Sulphur respiration
;
Electrochemical proton potential
;
Wolinella succinogenes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Wolinella succinogenes grown on formate and elemental sulphur was found to use the polysulphide derivatives 2,2′-tetrathiobispropionate (R2S4) or pentathionate (S5O 6 = ) as acceptors for formate oxidation. The specific activities of formate oxidation with these acceptors were similar to those with elemental sulphur. The main reaction products of R2S4 reduction were 2,2′-dithiobispropionate (R2S2) and sulphide. Pentathionate was converted to thiosulphate and some elemental sulphur. The electrochemical proton potential $$(\Delta \tilde \mu _H )$$ across the cytoplasmic membrane of the bacterium was measured in the steady state of electron transport from formate to R2S4. The electrical proportion (Δψ) of the $$(\Delta \tilde \mu _H )$$ determined through the distribution of labeled tetraphenylphosphonium cation was obtained as 0.17 Volt. The Δψ was zero, when a protonophore was present. The pH-difference across the membrane was negligible. Thus the $$(\Delta \tilde \mu _H )$$ generated by sulphur respiration is close to that measured earlier with fumarate as the terminal acceptor of electron transport.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425494
