ISSN:
1432-0983
Keywords:
Schizosaccharomyces pombe
;
Thialysine resistance
;
Lysine transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mutants defective in lysine transport were isolated and characterized. After UV-mutagenesis colonies resistant to thialysine, a toxic analogue of lysine, were isolated and L-lysine uptake into the mutant strains was analyzed. Among the thialysine-resistant strains a group of mutants was found, where the half-saturation constant, KT, of the high-affinity transport system for lysine was higher than in the wild-type, the high-affinity transport system for basic amino acids being specifically affected. This was confirmed by a complementation test in which all the thialysine-resistant strains with a higher KT for lysine uptake belonged to one complementation group. Kinetic and genetic analysis showed that our mutants were identical with can1-1 mutants, showing that a single high-affinity system for the transport of basic amino acids exists in S. pombe.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351694
