ISSN:
1432-0983
Keywords:
Heat shock
;
Recombinant DNA
;
Membrane protein
;
Nutritional limitation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 aminoacid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00312631
