ISSN:
1432-0983
Keywords:
Key words Schizophyllum commune
;
Mating type
;
Gene replacement
;
Gene disruption
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Gene X maps immediately right of gene Y at the Aα mating-type locus in Schizophyllum commune. Alleles X1, X3 and X4 were isolated, subcloned, and sequenced. The structure of the alleles and their relationship to the Aα locus is described. The deduced X isoforms possess no recognized motifs common to other polypeptides and no significant similarity to any sequences in the protein databases. X alleles do not activate A-regulated development when transformed into recipient strains possessing different Aα mating types or when these transformants are mated with tester strains. An X1-disrupted construction yielded a high frequency (33%) of homologous gene replacements. X-disrupted mutants have wild-type phenotypes and mate normally. Both the functional analyses and sequence data for X1, X3, and X4 suggest that the right boundary of the Aα mating-type locus falls between AαY and X. We propose that the Z and Y genes constitute the Aα locus in its entirety.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050029
