ISSN:
1432-0983
Keywords:
Key words Glutathione
;
Yap1
;
H2O2
;
t-BOOH
;
Glucose repression
;
Oxidative stress
;
Diauxic shift
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Resistance of haploid yeast to hydrogen peroxide and to tert-butylhydroperoxide strongly increases when 4% glucose is replaced by glycerol or ethanol as the carbon source of the complex medium. Using a GSH1-promoter-lacZ-fusion reporter construct we could demonstrate that GSH1 is one of the genes that are up-regulated during the shift from fermentative to oxidative metabolism. A gsh1 mutant did not exhibit respiratory growth resistance to H2O2, whereas it was only slightly impaired in acquiring resistance against t-BOOH in the same experimental conditions. An isogenic Δyap1 mutant, although more sensitive to oxidative stress than the wild-type (WT), could increase resistance to both peroxides by a similar factor as observed for the WT when shifted from 4% glucose to a non-fermentable carbon source. This indicates that in this case induction of resistance to oxidative stress is independent from Yap1 and from the Yap1-mediated stress response via the STRE motif.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050516
