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  • 1
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Rat fetus ; Immunocytology ; Hypothalamus ; ACTH, MSH, LPH, GH, Prolactin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ontogenesis of cells containing polypeptide hormones (ACTH, MSH, LPH, GH and Prolactin) was investigated in the fetal rat hypophysis by immunohistochemistry using the peroxidase-antiperoxidase complex. Corticotrophs, melanotrophs and lipotropic cells were revealed earlier in the pars distalis than in the pars intermedia. In the pars distalis, cells producing LPH were found in the morning of day 15 of gestation using anti-γ- or anti-β-LPH sera, and in the afternoon using anti-α- or β-endorphin sera. Cells containing β-MSH were observed from the afternoon of day 15. The cells stainable with the anti-α-MSH, anti-β-(17-39)ACTH and anti-β-(l-24)ACTH sera appeared on day 16. In the pars intermedia, the cells producing α-MSH, β MSH, α- and β-endorphin, γ and β-LPH were observed in the morning of day 17, while cells containing ACTH were only revealed in the afternoon of the same day of gestation. Based on the treatment of serial paraffin sections with various antisera, it was clearly shown that MSH, ACTH, and LPH occur in the same cells located in the pars distalis as in the pars intermedia. The development of the corticotrophs, melanotrophs and lipotropic cells does not require the presence of the fetal hypothalamus or other central nervous structures. The pituitary glands of 21 day-old fetuses encephalectomized on day 16 showed as many reactive cells as those of the littermate controls. The somatotrophs were first revealed in the pars distalis in the afternoon of day 19. The cells producing prolactin were not observed before day 21 of gestation. On some cases GH and prolactin were found together in one cell. The cytodifferentiation of GH and prolactin cells is apparently not under hypothalamic control.
    Type of Medium: Electronic Resource
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