ISSN:
1432-198X
Keywords:
Key words Histomorphometry
;
In situ hybridization histochemistry
;
Molecular morphometry
;
Immunohistochemistry
;
Bone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Quantitative histomorphometric assessment of bone biopsies represents a powerful and informative method for the study of metabolic bone diseases. It is the gold standard against which the noninvasive ”diagnostic” markers of bone metabolism as well as newly available therapeutic modalities are tested. With the rapid progress in technology of molecular biology, identification of systemic and local biomolecules known to regulate bone metabolism can now be achieved. The study of localization, levels of expression, and synthesis of these factors in bone and its microenvironment is possible through applications of in situ hybridization histochemistry (ISHH) and immunohistochemistry (IHC). Application of ISHH allows study of specific mRNA expression. IHC determines the presence and distribution of target protein in cells. These two methodologies provide the link between the cellular processes of mRNA transcription and translation to the working protein. Combining the established bone histomorphometric techniques with ISHH and IHC elevates the study of bone to new heights, i.e., cellular and molecular mechanistic issues can now be studied.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004670000345
