ISSN:
1435-4373
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract The aminoglycoside modifying enzyme aminoglycoside 3′- phosphotransferase II (APH(3′)II) is encoded for on transposon Tn5 by theaphA gene, in the same operon as theble gene determining bleomycin resistance. To document this linkage 82 kanamycin-resistantEscherichia coli strains of clinical origin were studied; all 18 isolates presenting bleomycin-kanamycin resistance were shown by an enzymatic assay to produce APH(3′)II, and the presence of Tn5 was demonstrated by gene hybridization. Similarly, bleomycin-kanamycin resistance was shown to be linked to APH(3′)II production inSalmonella spp. The epidemiology of strains with Tn5-encoded APH(3′)II may thus be studied, at least inEscherichia coli, by a simple diffusion test using bleomycin and kanamycin discs.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01967573