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  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The production of aerobactin has been suggested to be a virulence factor in Escherichia coli. We have studied the production of aerobactin in 155 E. coli strains, isolated from sewage, which carry conjugative antibiotic resistance plasmids, and 88 (57%) of these strains produced aerobactin, and 59 (38%) co-transferred production of the siderophore and antibiotic resistance. In 35 (22%) of the transconjugants, both characters seemed to be encoded into the same plasmid. Those aerobactin-producing-antibiotic resistance plasmids had different sizes and antibiotic resistance patterns. The ecological implications of these results are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aminoglycoside modifying enzyme aminoglycoside 3′- phosphotransferase II (APH(3′)II) is encoded for on transposon Tn5 by theaphA gene, in the same operon as theble gene determining bleomycin resistance. To document this linkage 82 kanamycin-resistantEscherichia coli strains of clinical origin were studied; all 18 isolates presenting bleomycin-kanamycin resistance were shown by an enzymatic assay to produce APH(3′)II, and the presence of Tn5 was demonstrated by gene hybridization. Similarly, bleomycin-kanamycin resistance was shown to be linked to APH(3′)II production inSalmonella spp. The epidemiology of strains with Tn5-encoded APH(3′)II may thus be studied, at least inEscherichia coli, by a simple diffusion test using bleomycin and kanamycin discs.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir berichten über fünf Fälle von intestinaler Kryptosporidiose mit pulmonaler Beteiligung bei AIDS Patienten. Die Diagnose wurde durch den Nachweis von säurefesten Oozysten im Sputum oder aspiriertem Bronchialsekret und im Stuhl gestellt. Bei allen Patienten bestand Husten und Auswurf. Bei vier Patienten fand sich gleichzeitig eine Assoziation mit anderen Infektionen, in zwei Fällen durchMycobacterium tuberculosis, in einem Fall durchMycobacterium fortuitum und in einem Fall durch Cytomegalovirus plusPneumocystis carinii. Bei allen Patienten war eine vorbestehende (drei Fälle) oder gleichzeitige intestinale Kryptosporidiose vorhanden, die mit Durchfall und Erbrechen einherging. Bei dem fünften Patienten war im Bronchialsekret ausschließlichCryptosporidium nachzuweisen, die Diarrhoe setzte erst einen Monat nach Entdeckung des Erregers im Respirationstrakt ein. Seit 1980 wurden 57 Fälle von respiratorischer Kryptosporidiose publiziert. In 17 Fällen fand sich kein anderer Erreger. In je 77% der Fälle waren Diarrhoe und Husten, in 58% Atemnot, in 54% Auswurf, 45% Fieber und in 33% Schmerzen aufgetreten.
    Notes: Summary Five case of intestinal cryptosporidiosis with pulmonary involvement in patients with AIDS are reported. The diagnosis was based on the recognition of acid-fast oocysts in sputum or aspirated bronchial material and stool specimens. Coughing and excess secretions were present in all cases. Four patients had other associated pulmonary pathogens: twoMycobacterium tuberculosis, oneMycobacterium fortuitum and one Cytomegalovirus +Pneumocystis carinii; all of them had a previous (three cases) or simultaneous (one case) diagnosis of intestinal cryptosporidiosis, presenting with diarrhoea and vomiting. In the fifth patientCryptosporidium was the only pulmonary pathogen found in a bronchial aspirate, and the onset of diarrhoea was 1 month after respiratory detection. Fifty-seven cases of respiratory cryptosporidiosis have been reported since 1980. In 17 of them, no other pathogen was found. Diarrhoea was present in 77% of the patients, cough in 77%, dyspnea in 58%, expectoration in 54%, fever in 45%, thoracic pain in 33%.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 19 (2000), S. 545-547 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The incidence of astrovirus infection in children less than 10 years of age with gastroenteritis in the city of Zaragoza, Spain, was analysed during a 12-month period. A total of 718 stool samples obtained from 534 children were examined. In 401 samples no routinely searched for pathogenic organism was detected; these specimens were then tested for the presence of astrovirus antigens. Astrovirus was detected in the samples of 15 (5.5%) patients, a detection rate similar to that recognised for Yersinia enterocolitica and Cryptosporidium spp. All children with confirmed astrovirus infection had diarrhoea (median duration, 9 days), 11 experienced loss of appetite, 5 vomiting, and 4 fever. The incidence of astrovirus infection reported here indicates that Spanish children suffering from gastroenteritis should be routinely screened for the presence of astrovirus.
    Type of Medium: Electronic Resource
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