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Arming of Lymphoid Cells by IgG Antibodies Treated with Protein A from Staphylococcus aureus (1976)

SULICA, A. ; LAKY, M. ; GHERMAN, M. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 5 (1976), S. 0

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ISSN: 1365-3083

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Mouse spleen lymphocytes treated with rabbit IgG anti-sheep erythrocytes (SRBC) complexed with protein A of Stapbylococcus aureus (SpA) form rosettes with SRBC. The attachment of SRBC to lymphocytes was due to the binding of the SpA-IgG antibody complex to the surface of the lymphocytes and was thus considered ‘arming’ of the cells. Normal mouse spleen cells ‘armed’ with SpA-rabbit IgG anti-chicken erythrocytes (CRBC) kill specifically 51Cr-labeled CRBC ‘in vitro’ in the absence of free antibodies. The killing by these ‘armed’ cells is an effect of the cell-bound SpA-IgG antibody complex. Both the SRBC rosette formation and the cell-mediated CRBC killing was dependent on the concentration of the SpA-IgG antibody complexes used for ‘arming’ the cell. A 100-fold increase in rosette formation or in killing of target cells was recorded for lymphocytes treated with SpA-IgG antibody complexes in comparison with cell treated with noncomplexed IgG antibodies. The specific binding of SpA-IgG antibody complexes to the Fc receptors of mouse spleen cells was demonstrated by inhibition studies. More than 60% inhibition of the rosette formation and in the killing of target cells was shown for cell treated With normal rabbit IgG or its Fc fragment before addition of the SpA-IgG antibody complex.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3083.1976.tb00262.x

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