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  • Articles: DFG German National Licenses  (17)
  • 2005-2009  (3)
  • 1990-1994  (14)
  • 1
    Electronic Resource
    Electronic Resource
    The detection of serum antibody to Renibacterium salmoninarum in pen-cultured coho salmon, Oncorhynchus kisutch (Walbaum) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 14 (1991), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1991.tb00594.x
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  • 2
    Electronic Resource
    Electronic Resource
    Productin of Basic Fetoprotein in Human Peripheral Lymphocytes during Blastic Transformation (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 36 (1992), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To clarify the significance of basic fetoprotein (BFP) in lymphocytes, we investigated whether BFP is produced in lymphocytes during blastic transformation. Peripheral blood lymphocytes obtained from 14 adults were cultured under the stimulation of lectins. The concentration of BFP in the culture medium (extracellular BFP) was estimated serially. The incorporation of [6-3 H] thymidine was assayed simultaneously. The intracellular BFP was measured by dual flow cytometry for DNA and BFP. A lymph node was studies immunohistochemically. Serum BFP was measured in four cases of lumphocytic leukaemia. In two cases, dual staining was performed. The intracellular BFP of the mitogen-stimulated lymphocytes was increased within 24 h. The extracellular BFP was increased exponentially from 72 h. The extracellular BFP at 96 h did not correlate with the [3H]-thymidine incorporation. The intracellular BFP increase began in G1 phase. Immunostaining showed that the B cells also produced BFP. Theserum BFP level in leukaemia was high in 1 of 4 cases and the leukaemic cells in two cases showed high intracellular BFP content. These observations indicate that BFP is produced in activated human lymphocytes and in lymphocytic leukaemic cells. The production of BFP during blastic transformation will be a useful new in vitro model for studying the biological role of BFP, and BFP labelling may offer some new possibilities for study of lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1992.tb03228.x
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  • 3
    Electronic Resource
    Electronic Resource
    Successful steroid pulse treatment in childhood acquired haemophilia with nephrotic syndrome (2005)
    Oxford, UK : Blackwell Science Ltd
    Haemophilia 11 (2005), S. 0 
    Details
    ISSN: 1365-2516
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary.  We encountered a 2-year-old boy with acquired haemophilia, which rarely occurs in children, who was complicated with nephrotic syndrome. In mid-August 2001, he was diagnosed to have nephrotic syndrome based on the presence of massive proteinuria and hypoalbuminaemia. Activated partial thromboplastin time (APTT) was normal at 42.4 s at that time. After starting prednisone administration of 2 mg kg−1 day−1, the proteinuria disappeared immediately. However, in early October the same year, subcutaneous ecchymosis and intramuscular bleeding occurred for no apparent reason, and from the examination results his APTT was 106.4 s, factor VIII (FVIII) activity was 〈1%, and the anti-FVIII inhibitor titre was 6.9 BU ml−1. As a result, he was diagnosed to have acquired haemophilia. The anti-nuclear antibody and anti-phospholipid antibody were negative. With recombinant activated FVII, haemostasis was obtained, and after administering three courses of steroid pulse therapy (methyl prednisolone: 20 mg kg−1 day−1 × 3 days), the anti-FVIII inhibitory activity disappeared. An analysis of the immunological and coagulation properties of his FVIII autoantibodies showed the anti-FVIII inhibitory activity to be mediated by IgG1 antibody. In other words, his FVIII inhibitor was a Th1 dominant and it provided a good response to treatment. These findings correlate with those of previous reports. The patient thereafter frequently demonstrated a recurrence of nephrotic syndrome. As a result, he is presently being managed with cyclosporine. However, no recurrence of the anti-FVIII titre has been observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2516.2005.01093.x
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  • 4
    Electronic Resource
    Electronic Resource
    Loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens (2005)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 28 (2005), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. Early diagnosis plays a vital role in management of fish and shellfish diseases. Traditionally, various biochemical and serological tests have been used for fish disease diagnosis. However, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. Polymerase chain reaction and probe-based nucleic acid detection have become increasingly popular in fish and shellfish diagnostics. Recently, a novel technique called loop-mediated isothermal amplification (LAMP) has been developed, which is highly sensitive and rapid. LAMP has been used for the detection of bacterial, viral, fungal and parasitic diseases in both animal and plants. In aquaculture, LAMP-based detection of pathogens like Edwardsiella tarda, E. ictaluri, Nocardia seriolae, Tetracapsuloides bryosalmonae, white spot syndrome virus and infectious haematopoietic necrosis virus have been reported. In this review, the application of LAMP for the detection of aquaculture-associated pathogens is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.2005.00670.x
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  • 5
    Electronic Resource
    Electronic Resource
    Enhancement of resistance to bacterial infection in rainbow trout, Oncorhynchus mykiss (Walbaum), by oral administration of bovine lactoferrin (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 16 (1993), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Bovine lactoferrin was evaluated for its ability to enhance resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), to infection with Vibrio anguillarum. Oral administration of lactoferrin (100 mg kg−1) to fish daily for 3 days prior to an intraperitoneal challenge with V. anguillarum resulted in increased survival rates, and enhanced resistance against Streptococcus sp., although to a lesser extent. In lactoferrin-treated fish, an increase in phagocytic and chemiluminescent (CL) activities of pronephros cells against V. anguillarum was observed. The phagocytic and CL activities of cells against Streptococcus sp. were also significantly increased. However, no in vitro bactericidal activities of lactoferrin against V. anguillarum or Streptococcus sp. were observed. This suggests that the lactoferrin enhanced the resistance of the rainbow trout against bacterial infection through the activation of phagocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1993.tb01253.x
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  • 6
    Electronic Resource
    Electronic Resource
    Immunomodulatory effects on rainbow trout, Oncorlynchus mykiss, injected with the extract of abalone, Haliotis discus hannai (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 7 (1991), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Rainbow trout, Oncorhynchus mykiss, injected with a water fraction extracted from abalone, Haliotis discus hannai, showed enhanced phagocytosis. This enhanced activity reached its maximum level 5 days after injection. The natural killer cell activities and the chemiluminescence responses of kidney leukocytes in fish treated with this fraction were also significantly increased, compared to those in albumin-treated fish. Rainbow trout injected intraperitoneally with this fraction showed an increased resistance to Vibrio anguillarum.〈section xml:id="abs1-2"〉〈title type="main"〉Zusammenfassung Immunomodulationseffekte bei Regenbogenforellen, Oncorhynchus mykiss, nach Injektion von Aba-lone-Entrakt, Haliotis discus hannaiBei Regenbogenforellen, Oncorynchus mykiss, denen ein wäßriger Extrakt der Abalone, Haliotis discus hannai, injiziert wurde, konnte eine gesteigerte Phagocytoseaktivität beobachtet werden. Diese Aktivi-tätssteigerung erreichte 5 Tage nach Verabreichung der Injektion ihr Maximum. Die natürliche Aktivi-tat von Killer-Zellen und die Chemoluminizenz-Reaktion der Nieren-Leukozyten in den mit dem Extrakt behandelten Fischen waren gegenüber Tieren, denen Albumin injiziert wurde, signifikant erhoht. Regenbogenforellen, denen der Abalone-Extrakt intraperitoneal injiziert wurde, zeigten eine erhohte Resistenz gegen Vibrio anguillarum.〈section xml:id="abs1-3"〉〈title type="main"〉Résumé Effects immunomodulatoires chez la truite arc-en-ciel, Onchorhynchus mykiss, traitie par des injections d'extraits d'onneaux, Haliotis discus hannai La truite arc-en-ciel, Onchorhynchus mykiss, traitie par injection d'une fraction aqueuse extraite d'ormeaux, Haliotis discus hannai, a montré une augmentation du taux de phagocytose. Cette augmentation de I'activité a atteint son niveau maximal cinq jours aprés injection. Les activitts naturelles des cellules tueuses et les résonses chimioluminescentes des leucocytes du rein des poissons traitis par cette fraction ont aussi significativement augmentis, par rapport ä celle des poissons traitis par de l'albumine. Les truites arc-en-ciel uaities par des injections intrapiritoniales de cette fraction ont montré une résistance accrue äVibrio anguillarum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0426.1991.tb00594.x
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  • 7
    Electronic Resource
    Electronic Resource
    Pharmacokinetics of flosequinan in elderly patients with chronic congestive heart failure (1993)
    Springer
    European journal of clinical pharmacology 44 (1993), S. 387-389 
    Details
    ISSN: 1432-1041
    Keywords: Flosequinan ; Congestive heart failure ; elderly ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary We have investigated the pharmacokinetics of the direct vasodilator flosequinan in elderly patients with congestive heart failure. Eight patients received a single dose of 50 mg, and 8 patients received once-daily treatment with 25 mg for two weeks. In the single dose study, the tmax of flosequinan was 2.5 h, Cmax was 1.17 μg · ml−1 and t1/2 was 5.63 h. The tmax of the metabolite BTS 53554 was 20.3 h, Cmax was 1.44 μg · ml−1 and t1/2 was 62.0 h. BTS 53554 accumulated gradually in the 14-day repeated dose study and steady-state was reached after approximately 2 weeks. Flosequinan was not found to accumulate. Adverse reactions were not observed in either the single or repeated dose study. It is advisable to consider renal function and body weight when flosequinan is to be administered to elderly patients with congestive heart failure. The initial dose should be 25 mg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316479
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  • 8
    Electronic Resource
    Electronic Resource
    Micro-Columnar Crystals with (-O=V-Pd-)〈sub〉n〈/sub〉 Infinite Chain Structures Assembled by Hetero-Dinuclear Metal Complexes (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 11-12 (Feb. 2006), p. 273-276 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The inorganic and organic hybrid compound, [O=VPd(2-Pyd-)4]n (2-Pyd- = 2-pyridonate)(1), was synthesized by assembling its monomer units in the organic solution. X-ray absorptionspectroscopic (XAFS) studies indicated that oxidation states of the vanadium and palladium ions in 1were +IV and +II, and the distance between them was estimated to be 2.698-2.700 Å. Scanningelectron microscope (SEM) observation revealed that the compound 1 shapes in long columnarcrystals with micrometer-sized cross section. EXAFS and IR studies suggested that the O=V oxygenatom interacted to the central palladium ion in the neighboring monomer unit, forming the inorganicinfinite chain structure, -(O=V-Pd)n-, in the columnar crystals of 1
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.11-12.273.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Detection of tyrosine hydroxylase and phenylethanolamine-N-methyltransferase messenger RNAs in the mouse adrenal gland and the brain by in situ hybridization (1992)
    Springer
    Histochemistry and cell biology 97 (1992), S. 201-206 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To study the expression of tyrosine hydroxylase (TH) and phenylethanolamine-N-methyltransferase (PNMT) genes in the mouse adrenal gland and the brain, we performed in situ hybridization studies by using several types of complementary DNA probes recognizing coding regions of human TH (THc), the 3′-end of the human region of TH (TH3′), and the coding region of the human PNMT (PNMTc). THc mRNA was detected in the chromaffin cells of the mouse adrenal medulla and brain catecholaminergic neurons including the substantia nigra and ventral tegmental area. TH-immunopositive neurons were located in a similar pattern in adjacent sections. However no positive signals were detected by the TH3′ probe. Using the PNMTc probe, the majority of cells in the adrenal medulla demonstrated positive labelling. Although the mouse TH and PNMT genes have not been fully isolated and sequenced, the present study strongly suggests that the sequence of the coding regions of TH and PNMT are similar in human and mouse. The THc and PNMTc probes are particularly useful in investigating the loci of gene transcription in mouse tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267628
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  • 10
    Electronic Resource
    Electronic Resource
    A criterion for the positivity of rational cubicC 2-spline interpolants (1990)
    Springer
    Computing 44 (1990), S. 365-368 
    Details
    ISSN: 1436-5057
    Keywords: 65D07 ; 41A15 ; Positive spline interpolation ; sufficient and necessary existence condition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Es wird eine hinreichend und notwendige Bedingung für die Positivität von rational-kubischen Splines angegeben, welche sich leicht überprüfen läßt. Sie ist bei Interpolationsdaten mit positiven Ordinaten durch hinreichend große Rationalitätsparameter stets erfüllbar.
    Notes: Abstract By means of a finite set of inequalities a sufficient and necessary condition for the positivity of rational cubic splines is given which holds true whenever the rationality parameters are sufficiently large.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02241274
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