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  • Articles: DFG German National Licenses  (2)
  • Flagellum  (1)
  • Hypha  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Rapid wound responses ofSaprolegnia ferax hyphae depend upon actin and Ca2+-involving deposition of callose plugs (2000)
    Springer
    Protoplasma 214 (2000), S. 199-209 
    Details
    ISSN: 1615-6102
    Keywords: Actin ; Callose ; Coenocyte ; Hypha ; Membrane potential ; Oomycetes ; Wound response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Growing hyphae of the oomyceteSaprolegnia ferax wounded by impalement with a ca. 0.2 μm diameter glass microelectrode normally respond within seconds with an apically directed cytoplasmic contraction followed by production of a plug which encases the electrode and occludes its recording of transmembrane potentials. This plug contains callose and Ca2+-associated membranes. To characterize the rapid wounding response, we disrupted specific filamentous (F) actin populations and Ca2+ regulation. Plug formation is inhibited by disruption of F-actin populations and low exogenous Ca2+ but not by inhibition of stretch-activated Ca2+ channels with Gd3+. Therefore, stretch-activated channels are not the immediate sensor. Instead, sensing may involve strain on the actin cytoskeleton which triggers the occlusion response. This wound response is qualitatively similar to the production of septa which isolate developing sporangia and seal severed hyphae, indicating the use of a normal basic cellular developmental system as a protective mechanism against environmental damage. The wound response is essential, since an inability to seal sites of mechanical damage is potentially catastrophic in acellular coenocytic organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279064
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Observations on the ultrastructure of microtubules (1971)
    Springer
    Cell & tissue research 115 (1971), S. 388-395 
    Details
    ISSN: 1432-0878
    Keywords: Microtubule ; Ultrastructure ; Spindle ; Flagellum ; Subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After a specific time of glutaraldehyde-acrolein fixation, microtubule walls appear to be composed of single 6.5–7.5 nm diameter osmiophilic subunits. Variations in the duration of glutaraldehyde-acrolein and also glutaraldehyde-osmium fixation reveal a two layered wall containing osmiophilic subunits, 4.0–4.5 nm in diameter, arranged radially, in tandem. The double-layered wall is demonstrated by microdensitometer traces. These observations are discussed in relation to previously proposed models of microtubule substructure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324941
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    Paper (German National Licenses)
    Fulltext
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