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  • Articles: DFG German National Licenses  (6)
  • Life and Medical Sciences  (4)
  • Prognose  (2)
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  • Articles: DFG German National Licenses  (6)
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Years
  • 1
    ISSN: 1432-1963
    Keywords: Schlüsselwörter DNA-Zytophotometrie ; Mammakarzinom ; Prognose ; Key words DNA-flow cytometry ; Breast cancer ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary DNA flow cytometry (FCM) has become a routine method in breast cancer diagnosis for evaluation of ploidy and proliferation kinetics (cell cycle analysis). Image cytometry is less practicable and provides less information than flow cytometry. An optimized technique with a low coefficient of variation is required for optimal results in flow cytometry. The S-phase fraction and the proliferation index (sum of S-phase fraction and G2M fraction) provide prognostic and therapeutically relevant information. A profound knowledge of the technique and its limitations is indispensable for the interpretation of FCM results. It remains to be established whether immunohistological evaluation of cell proliferation has the same prognostic value. Future developments are to be expected from multiparametric analysis and the improvement of mathematical analysis of FCM measurements.
    Notes: Zusammenfassung Die DNA-Zytometrie hat sich zu einer diagnostischen Routinetechnik entwickelt, die bevorzugt beim Mammakarzinom zur Beurteilung der Ploidie und der Proliferationskinetik (Zellzyklusanalyse) eingesetzt wird. Dabei ist die statische Zytometrie (Mikroskopzytometrie) aus Gründen der geringeren Aussagekraft der Durchflußzytometrie unterlegen. Die Durchflußzytometrie erfordert zur S-Phasen-Bestimmung eine standardisierte und methodisch einwandfreie Technik mit einem niedrigen Variationskoeffizienten (CV). Die gewonnenen Parameter, in 1. Linie die S-Phase und der Proliferationsindex, in 2. Linie auch die Ploidie, stellen prognostisch und für die Therapieplanung wichtige Faktoren dar. Eine genaue Kenntnis der Methode und der Fehlermöglichkeiten ist für die Interpretation der Ergebnisse unerläßlich. Inwieweit immunhistologische Techniken zur Bestimmung der Proliferationskinetik gleichwertige Informationen liefern, muß zum gegenwärtigen Zeitpunkt als ungeklärt angesehen werden. Weitere Entwicklungen betreffen die Einführung von multiparametrischen Untersuchungen und Verbesserung der mathematischen Analyse der Meßwerte.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Mammakarzinom ; Prognose ; Grading ; Key words Breast cancer ; Prognosis ; Grading
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Invasive lobular carcinoma (ILC) is recognized in its classical form and as variants with tubulo-lobular, solid, pleomorphic, alveolar or signet ring cell differentiation. The most common classical form differs from invasive ductal carcinoma (IDC) by its slower tumor proliferation and less common axillary metastases. When compared stage by stage, long term prognosis is similar to IDC, however. Prognostic subtyping of ILC can be achieved by the recognition of variant forms and mitotic counting. The combination of these factors may be used for tumor grading (5-year survival 100 % with grade 1 vs. 82 % with grade 2, and 57 % with grade 3, n = 241). The detailled histopatholgic diagnosis therefore permits prognostic assesment also in ILC.
    Notes: Zusammenfassung Das invasive lobuläre Karzinom (ILC) schließt die klassische Form und Varianten mit tubulo-lobulärer, solider, pleomorpher, alveolärer oder siegelringzelliger Differenzierung ein. Die häufigste klassische Form unterscheidet sich vom invasiven duktalen Karzinom (IDC) durch eine langsamere Tumorproliferation und seltenere lymphogene Metastasierung, weist jedoch stadienbezogen eine ähnliche Langzeitprognose wie das IDC auf. Durch Abgrenzung der varianten Formen gegenüber dem klassischen Subtyp und Berücksichtigung der Mitoseaktivität läßt sich das ILC in prognostisch unterschiedliche Gruppen einteilen und ein Gradingsystem aufstellen (5-Jahres Überlebensraten 100 % bei Grad 1 gegenüber 82 % bei Grad 2 und 57 % bei Grad 3, n = 241). Eine detaillierte histopathologische Diagnostik ermöglicht somit auch beim ILC eine genauere Abschätzung des biologischen Verhaltens dieser Tumoren.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 70 (1967), S. 265-273 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The kinetics of RNA synthesis have been studied under various growth conditions using synchronized Tetrahymena pyriformis GL. The curves obtained are approximately linear in growth-supporting media but vary under other circumstances. The presence of amino acids in the medium stimulates RNA synthesis in Tetrahymena as it does in bacteria. Since these induced variations do not influence the essential features of the pre-cytokinetic period it is inferred that fluctuations in the rate of RNA synthesis described by others are probably not essential features of temperature-induced synchrony. In addition, the translational stability of division-related templates at synchronizing temperatures has been investigated. The synthesis of proteins necessary for cytokinesis is shown to be greatly reduced under conditions simulating a heat-shock. Inhibitor studies using cycloheximide indicate that protein synthesis is required longer into the cytokinetic phase than had previously been thought. Collectively the data are all compatible with the hypothesis that synthesis of division-related proteins is drastically reduced in heat-shocked Tetrahymena and that the basis for this reduction is hydrolysis of template RNA without concurrent translation.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intracellular ribonuclease from the ciliate Tetrahymena pyriformis GL was purified 10-fold. After preheating for 20 minutes at 100°C of the ribonuclease preparation 80% of its activity was lost. Preheating under the same conditions, however, in the presence of RNA, did not affect the enzyme activity. Between 0°C and 41°C the apparent activation energy was 15,600 cal per deg. per mole.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 68 (1966), S. 203-205 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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