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  • Electronic Resource  (21)
  • 2000-2004  (10)
  • 1965-1969  (7)
  • 1955-1959  (3)
  • 1880-1889  (1)
  • 11
    Electronic Resource
    Electronic Resource
    Receptor-mediated endocytosis of Neisseria gonorrhoeae into primary human urethral epithelial cells: the role of the asialoglycoprotein receptor (2001)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 42 (2001), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Urethral epithelial cells are invaded by Neisseria gonorrhoeae during gonococcal infection in men. To understand further the mechanisms of gonococcal entry into host cells, we used the primary human urethral epithelial cells (PHUECs) tissue culture system recently developed by our laboratory. These studies showed that human asialoglycoprotein receptor (ASGP-R) and the terminal lactosamine of lacto-N-neotetraose-expressing gonococcal lipooligosaccharide (LOS) play an important role in invasion of PHUECs. Microscopy studies showed that ASGP-R traffics to the cell surface after gonococcal challenge. Co-localization of ASGP-R with gonococci was observed. As ASGP-R-mediated endocytosis is clathrin dependent, clathrin localization in PHUECs was examined after infection. Infected PHUECs showed increased clathrin recruitment and co-localization of clathrin and gonococci. Preincubating PHUECs in 0.3 M sucrose or monodansylcadaverine (MDC), which both inhibit clathrin-coated pit formation, resulted in decreased invasion. N. gonorrhoeae strain 1291 produces a single LOS glycoform that terminates with Gal(β1–4)GlcNac(β1–3)Gal(β1–4)Glc (lacto-N-neotetraose). Invasion assays showed that strain 1291 invades significantly more than four isogenic mutants expressing truncated LOS. Sialylation of strain 1291 LOS inhibited invasion significantly. Preincubation of PHUECs in asialofetuin (ASF), an ASGP-R ligand, significantly reduced invasion. A dose–response reduction in invasion was observed in PHUECs preincubated with increasing concentrations of NaOH-deacylated 1291 LOS. These studies indicated that an interaction between lacto-N-neotetraose-terminal LOS and ASGP-R allows gonococcal entry into PHUECs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2001.02666.x
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  • 12
    Electronic Resource
    Electronic Resource
    EFFECT OF CERTAIN CHEMICALS IN WATER ON THE FLAVOR OF BREWED COFFEE (1958)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 23 (1958), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1958.tb17606.x
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    Paper (German National Licenses)
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  • 13
    Electronic Resource
    Electronic Resource
    USE OF HIGH-VOLTAGE CATHODE RAYS TO DESTROY BACTERIA OF THE SALMONELLA GROUP IN WHOLE EGG SOLIDS, EGG YOLK SOLIDS, AND FROZEN EGG YOLK (1957)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 22 (1957), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1957.tb17518.x
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  • 14
    Electronic Resource
    Electronic Resource
    Fuss, Peter: The Moral Philosophy of Jonah Royce : London, Harvard University Press, 1965 (1966)
    Cambridge : Cambridge University Press
    Philosophy 41 (1966), S. 188-189 
    Details
    ISSN: 0031-8191
    Source: Cambridge Journals Digital Archives
    Topics: Philosophy
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1017/S0031819100058617
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  • 15
    Electronic Resource
    Electronic Resource
    Minimal Presentations and Efficiency of Semigroups (2000)
    Springer
    Semigroup forum 60 (2000), S. 231-242 
    Details
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: S is said to be efficient if it can be defined by a presentation (A | R) with |R| -|A|=rank(H 2(S)). In this paper we demonstrate certain infinite classes of both efficient and inefficient semigroups. Thus, finite abelian groups, dihedral groups D 2 n with n even, and finite rectangular bands are efficient semigroups. By way of contrast we show that finite zero semigroups and free semilattices are never efficient. These results are compared with some well-known results on the efficiency of groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002339910016
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  • 16
    Electronic Resource
    Electronic Resource
    Particle-mediated gene transfer to rat neurons in primary culture (2000)
    Springer
    Pflügers Archiv 439 (2000), S. 730-738 
    Details
    ISSN: 1432-2013
    Keywords: Dorsal root ganglion neurons Gene gun Green fluorescent protein Hippocampal neurons Particle-mediated gene transfer Primary tissue culture Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Gene transfer into neuronal cells provides an important approach to study their function. Particle-mediated gene delivery was used to transfect rat dorsal root ganglion (DRG) and hippocampal neurons in primary culture with the genes for the enhanced blue and green fluorescent proteins (EBFP and EGFP) under control of the cytomegalovirus promoter. Quantitative analysis of marker protein fluorescence detected expression at 3 h that continued to increase for 48 h. For DRG neurons the optimal expression efficiency of 8±2% was obtained 24 h following transfection. In contrast, approximately 2±1% of hippocampal neurons in culture expressed EGFP at 3 h which subsequently declined. Co-transfection of DRG cultures with two plasmids produced reliable expression of both genes. Transfected DRG neurons exhibited normal electrophysiological properties, and resting and stimulated intracellular Ca2+ concentrations were unchanged. After transfection, 44% of hippocampal neurons remained in functional synaptic networks as indicated by glutamatergic Ca2+ spiking activity. Particle-mediated gene delivery provided a straightforward, reproducible and efficient method for transfection of neurons in primary culture. Transfected cells were easily identified by EGFP fluorescence, enabling subsequent physiological analysis. Biolistic particle bombardment was well tolerated by peripheral neurons, although caution was required when this method was applied to CNS cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900240
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  • 17
    Electronic Resource
    Electronic Resource
    A first estimate of organic carbon storage in Holocene lake sediments in Alberta, Canada (2000)
    Springer
    Journal of paleolimnology 24 (2000), S. 395-400 
    Details
    ISSN: 1573-0417
    Keywords: carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008103605817
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  • 18
    Electronic Resource
    Electronic Resource
    Microbial Biomass and Community Structure in a Sequence of Soils with Increasing Fertility and Changing Land Use (2000)
    Springer
    Microbial ecology 40 (2000), S. 223-237 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microbial biomass and community structure of eight Chinese red soils with different fertility and land use history was investigated. Two community based microbiological measurements, namely, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid fatty acid (PLFA) profiles, were used to investigate the microbial ecology of these soils and to determine how land use alters microbial community structure. Microbial biomass-C and total PLFAs were closely correlated to organic carbon and total nitrogen, indicating that these soil microbial measures are potentially good indices of soil fertility in these highly weathered soils. Metabolic quotients and C source utilization were not correlated with organic carbon or microbial biomass. Multivariate analysis of sole carbon source utilization patterns and PLFAs demonstrated that land use history and plant cover type had a significant impact on microbial community structure. PLFAs showed these differences more than CLPP methods. Consequently, PLFA analysis was a better method for assessing broad-spectrum community differences and at the same time attempting to correlate changes with soil fertility. Soils from tea orchards were particularly distinctive in their CLPP. A modified CLPP method, using absorbance readings at 405 nm and different culture media at pH values of 4.7 and 7.0, showed that the discrimination obtained can be influenced by the culture conditions. This method was used to show that the distinctive microbial community structure in tea orchard soils was not, however, due to differences in pH alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002480000053
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  • 19
    Electronic Resource
    Electronic Resource
    Virulence, adsorbability, and antigenicity of foot-and-mouth disease virus selected by adsorption with homogenized mouse kidney (1969)
    Springer
    Archives of virology 26 (1969), S. 238-248 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A small percentage of a stock foot-and-mouth disease virus population was found to be relatively resistant to adsorption with homogenized adult mouse kidney. To select this portion of the population, a series of 10 passages was performed, each passage consisting of adsorption with homogenized kidney and propagation of the unadsorbed virus in bovine kidney cell cultures. The selected virus was relatively resistant to adsorption with mouse kidney and relatively avirulent for adult mice. It was immunogenic in adult mice and seemed to be antigenically the same as the parent virus. This information might possibly be used in selecting viruses for live virus vaccines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242376
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  • 20
    Electronic Resource
    Electronic Resource
    Corrosion rate measurements in vivo (1967)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 1 (1967), S. 405-414 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Corrosion resistance is particularly important in medical treatments requiring implantation of metallic objects in the human body. Unfortunately, weight loss measurements and other conventional corrosion testing techniques are insufficiently sensitive and difficult to adapt to in vivo environments. The linear polarization technique can be used to remotely meaure the corrosion rates of metals implanted in tissue using needle probes. The principles of this technique are reviewed and the results of tests performed on steel, cobalt, and molybdenum implanted in experimental animals are discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820010405
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