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  • 1
    Electronic Resource
    Electronic Resource
    A method for processing free-floating cultured cells for scanning electron microscopy (1982)
    Springer
    Methods in cell science 7 (1982), S. 85-88 
    Details
    ISSN: 1573-0603
    Keywords: specimen processing ; scanning electron microscopy ; aggregate cultures ; isolated cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and convenient method is described to aid the processing of minute tissue specimens and isolated single cells for scanning electron microscopy (SEM). The technique employs an inexpensive, disposable carrier fashioned from common Beem capsules and Nitex nylon screen. This method avoids excessive handling, transfers or centrifugation steps that may damage delicate specimens. Specimens processed by this method show excellent preservation of cell surface fine structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665947
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  • 2
    Electronic Resource
    Electronic Resource
    Submersion organ culture of preglandular fetal lung (1983)
    Springer
    Methods in cell science 8 (1983), S. 59-62 
    Details
    ISSN: 1573-0603
    Keywords: organ culture ; fetal rat lung ; submersion culture ; hydrated collagen gel ; pulmonary epithelium ; cytodifferentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This report describes a method for organ culture of the fetal rat lung at the early preglandular phase of development. When the intact fetal lung is cultured fully submerged in medium it develops as an expanded hollow organ. This pattern of in vitro morphogenesis provides a specimen uniquely suited to analysis of the pulmonary epithelium by scanning electron microscopy. In this system alveolar Type II cells and cell types of the distal pulmonary airways undergo differentiation in serum-free media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099239
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A new morphological procedure for viewing microvessels: A scanning electron microscopic study of the vasculature of small intestine (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 203 (1982), S. 493-503 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The present study details a new method for the exposure and viewing of individual microvessels located within the small intestine of rats. This procedure will selectively and consistently remove the outer muscle layers and underlying submucosa of the intestinal wall and thereby expose a variety of arterioles in their normal location within the tissue, with their normal relationship to each other undisturbed. The small intestine of the rat was initially fixed by vascular perfusion with 2.5% glutaraldehyde in 0.1 M cacodylate/HCL buffer, reinfused with heparinized whole blood, removed from the animal, and secured to a dissecting petri dish for further fixation. Subsequently, the external muscularis was dissected from the sample which exposed the submucosa. In order to remove the connective tissue elements from this layer and uncover the submucosal vasculature, the samples were first transferred to a solution of 30% potassium hydroxide for 2-5 minutes and then to a final digesting solution containing collagenase. Thereafter, the samples were routinely processed for light microscopy and for scanning (SEM) or transmission (TEM) electron microscopy. Examination of the samples revealed excellent preservation of the three-dimensional organization of the arteriolar wall with minimal membrane damage. This new technique now makes it possible to visualize the shape and position of individual smooth muscle cells along arterioles of differing size and branching order.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092030409
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructure of the developing vascular system in the puppy kidney (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 199 (1981), S. 481-489 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The present study defined the ultrastructural features of peritubular capillary development. Two-day-old beagle puppies and adult dogs were perfused with 2.5% glutaraldehyde and routinely prepared for light and transmission electron microscopy. Some of the fixed tissue was subsequently used to make freeze-fracture replicas.The outer cortex of the puppy kidney possessed large, thick-walled vessels best termed sinusoidal capillaries instead of the small caliber vessels (peritubular capillaries) noted in the adult. These sinusoidal vessels showed extensive overlapping of the endothelium with isolated patches of fenestrae. Their luminal surfaces were irregular, owing to prominent ridges and sporadic bulges of endothelium. The basement membrane of most vessels was not present. Interstitial spaces were filled with mesenchymal cells and cells closely resembling pericytes. The diameter of the fenestrae of vessels throughout the cortex was similar; however, the number of fenestrae per micrometer of endothelium increased significantly from outer to inner cortex. Vessels of the inner cortex were also immature when compared to the adult. From these morphological findings, it was apparent that a true peritubular capillary system does not exist in the two-day-old puppy. Ultrastructural features of these vessels suggested reduced permeability characteristics.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091990405
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of alloxan, and alloxan-induced diabetes on the kidney (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 208 (1984), S. 33-47 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Alloxan is known to induce diabetic renal changes as well as causing nephrotoxic alterations. However, no ultrastructural study has been performed to differentiate diabetic verses toxic affects of alloxan to the tubule and/or glomerulus. Therefore the present study used the “protected” kidney model to prevent one kidney from being exposed to the alloxan while allowing the other to receive the drug immediately. In all experimental animals the right renal hilum was gently occluded for 5 minutes and then released. This was performed prior to the injection of alloxan. Subsequently, the left renal hilum was occluded at the time of, and for 5 minutes after, alloxan administration (40 mg/kg i.v.). The experimental rats were divided into three groups: untreated diabetics, diabetics treated with protamine-zinc-insulin, and alloxantreated rats that failed to become diabetic. Three groups of controls were included: one group received an equal volume of saline diluent as the experimental rats but no clamping of either renal hilum; another group received the saline and had the left renal hilum occluded for 5 minutes; and a third group had both the right and left renal hila occluded. All animals were followed and sacrificed after 9 weeks. Endogenous creatinine clearance did not change among groups. Alloxan-treated nondiabetic rats displayed marked interstitial nephritis in unprotected kidneys, while protected kidneys were normal. The diabetic state resulted in mesangial proliferation and focal glomerular basement membrane thickening as well as glomerular capilary endothelial abnormalities and visceral epithelial foot-process fusion. The endothelial changes consisted of focal areas showing a reduction in the size of endothelial fenestrae. All glomerular changes were ameliorated by insulin treatment. We conclude: (1) alloxan per se is distinctly nephrotoxic; and (2) the glomerular endothelium and epithelium are involved early in the course of experimental diabetes.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092080105
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    Paper (German National Licenses)
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