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  • 2000-2004  (5)
  • 1995-1999
  • 1990-1994  (6)
  • 1880-1889
  • 2000  (5)
  • 1990  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Fluid Mechanics 22 (1990), S. 57-90 
    ISSN: 0066-4189
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Physical Chemistry 41 (1990), S. 775-837 
    ISSN: 0066-426X
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In the present study, we show that Neisseria gonorrhoeae lipooligosaccharide (LOS) can bind to the asialoglycoprotein receptor (ASGP-R) on human sperm. This work demonstrates the presence of ASGP-R on human sperm. Binding of purified ASGP-R ligand decreased in the presence of gonococci. Binding of purified iodinated gonococcal LOS identified a protein of molecular weight corresponding to that of human ASGP-R. The presence of excess unlabelled LOS blocked binding of iodinated gonococcal LOS. Binding of wild-type gonococcal LOS to sperm was higher than that of mutant LOS lacking the galactose ligand for ASGP-R. These data suggest that the ASGP-R on human sperm cells recognizes and binds wild-type gonococcal LOS. This interaction may contribute to the transmission of gonorrhea from infected males to their sexual partners.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. The following extracellular enzymes have been readily detected in the culture filtrates from Aeromonas salmonicida: amylase, phospholipase, lysophospholipase and ribonuclease. Amylase and phospholipase have been partially characterized. Evidence suggests that glycogen may be the natural substrate for amylase, and that the role of the enzyme in natural infection is to digest glycogen present in fish muscle. The secretion of amylase activity is suppressed by the addition of glucose to the growth medium. The amounts of amylase, phospholipase and protease that can be detected in culture filtrates decreases with increase in the growth temperature from 25 to 32°C. This marked decrease in secretion of hydrolytic enzymes occurs although the initial growth rates at 25 and 32°C are similar. Free and membrane associated ribosomes have been isolated from cultures grown at 25 and 32°C. At 32°C there is a smaller proportion of membrane-associated ribosomes and this is consistent with the hypothesis that extracellular enzymes from Aeromonas salmonicida are secreted on membrane-bound polysomes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-0417
    Keywords: carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 439 (2000), S. 730-738 
    ISSN: 1432-2013
    Keywords: Dorsal root ganglion neurons Gene gun Green fluorescent protein Hippocampal neurons Particle-mediated gene transfer Primary tissue culture Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Gene transfer into neuronal cells provides an important approach to study their function. Particle-mediated gene delivery was used to transfect rat dorsal root ganglion (DRG) and hippocampal neurons in primary culture with the genes for the enhanced blue and green fluorescent proteins (EBFP and EGFP) under control of the cytomegalovirus promoter. Quantitative analysis of marker protein fluorescence detected expression at 3 h that continued to increase for 48 h. For DRG neurons the optimal expression efficiency of 8±2% was obtained 24 h following transfection. In contrast, approximately 2±1% of hippocampal neurons in culture expressed EGFP at 3 h which subsequently declined. Co-transfection of DRG cultures with two plasmids produced reliable expression of both genes. Transfected DRG neurons exhibited normal electrophysiological properties, and resting and stimulated intracellular Ca2+ concentrations were unchanged. After transfection, 44% of hippocampal neurons remained in functional synaptic networks as indicated by glutamatergic Ca2+ spiking activity. Particle-mediated gene delivery provided a straightforward, reproducible and efficient method for transfection of neurons in primary culture. Transfected cells were easily identified by EGFP fluorescence, enabling subsequent physiological analysis. Biolistic particle bombardment was well tolerated by peripheral neurons, although caution was required when this method was applied to CNS cultures.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Semigroup forum 60 (2000), S. 231-242 
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: S is said to be efficient if it can be defined by a presentation (A | R) with |R| -|A|=rank(H 2(S)). In this paper we demonstrate certain infinite classes of both efficient and inefficient semigroups. Thus, finite abelian groups, dihedral groups D 2 n with n even, and finite rectangular bands are efficient semigroups. By way of contrast we show that finite zero semigroups and free semilattices are never efficient. These results are compared with some well-known results on the efficiency of groups.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of thermal analysis and calorimetry 36 (1990), S. 1669-1674 
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es werden thermische und optische Studien einiger Diacethylene beschrieben, die zwecks der Senkung der Temperatur für das Auftreten von Mesophasen eigens gestaltet wurden. Ursprüngliche Untersuchungen an symmetrischen Molekülen wurden erweitert, um auch typische Flüssigkeitskristallmoleküle mit Diacethylenen mit Polymerhauptketten und unsymmetrisch substituierte Diacethylene wie Schiff-Basen mit langen Alkylketten einzubeziehen.
    Notes: Abstract We describe the thermal and optical studies of some diacetylene molecules which were specially designed in an attempt to reduce the temperature at which the mesophase appears. Original studies were on symmetrical molecules and these were extended to include typical liquid crystal molecules with diacetylenes providing a polymeric backbone and unsymmetrically substituted diacetylenes including Schiffs bases with long alkyl chains.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-1561
    Keywords: Pheromone ; sex ; attractant ; aphid ; Phorodon humuli ; Homoptera ; Aphididae ; nepetalactol ; monoterpenoid ; electrophysiology ; single cell recording ; mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Behavioral studies using an olfactometer demonstrated that sexual females (oviparae) of the damson-hop aphid,Phorodon humuli, release a pheromone to which males respond. Volatiles produced by the oviparae were analyzed by coupled gas chromatography-single cell recording from the secondary rhinaria on the male antenna and showed the presence of one peak with major activity. Coupled gas chrornatography-mass spectrometry suggested a nepetalactol, which was shown to have the 4aR, 7S, 7aS stereochemistry by synthesis from the corresponding nepetalactone isolated from the labiate plantNepeta mussinii. Although the stereochemistry at carbon-1 is not yet established, a synthetic sample comprising ca. 70% 1S and 30% 1R attracted highly significant numbers of males to water traps placed within and adjacent to a hop garden. Initial studies also indicated attraction of males in both the olfactometer and in the field by volatiles from the primary host.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The microbial biomass and community structure of eight Chinese red soils with different fertility and land use history was investigated. Two community based microbiological measurements, namely, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid fatty acid (PLFA) profiles, were used to investigate the microbial ecology of these soils and to determine how land use alters microbial community structure. Microbial biomass-C and total PLFAs were closely correlated to organic carbon and total nitrogen, indicating that these soil microbial measures are potentially good indices of soil fertility in these highly weathered soils. Metabolic quotients and C source utilization were not correlated with organic carbon or microbial biomass. Multivariate analysis of sole carbon source utilization patterns and PLFAs demonstrated that land use history and plant cover type had a significant impact on microbial community structure. PLFAs showed these differences more than CLPP methods. Consequently, PLFA analysis was a better method for assessing broad-spectrum community differences and at the same time attempting to correlate changes with soil fertility. Soils from tea orchards were particularly distinctive in their CLPP. A modified CLPP method, using absorbance readings at 405 nm and different culture media at pH values of 4.7 and 7.0, showed that the discrimination obtained can be influenced by the culture conditions. This method was used to show that the distinctive microbial community structure in tea orchard soils was not, however, due to differences in pH alone.
    Type of Medium: Electronic Resource
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