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  • 2005-2009  (1)
  • 2000-2004  (1)
  • 1990-1994  (3)
  • 1
    Electronic Resource
    Electronic Resource
    350 Main Street , Malden , MA 02148 , USA , and 9600 Garsington Road , Oxford OX4 2DQ , UK . : Blackwell Publishing, Inc.
    Risk analysis 24 (2004), S. 0 
    ISSN: 1539-6924
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Adverse events in hospitals, such as in surgery, anesthesia, radiology, intensive care, internal medicine, and pharmacy, are of worldwide concern and it is important, therefore, to learn from such incidents. There are currently no appropriate tools based on state-of-the art models available for the analysis of large bodies of medical incident reports. In this study, a new model was developed to facilitate medical error analysis in combination with quantitative risk assessment. This model enables detection of the organizational factors that underlie medical errors, and the expedition of decision making in terms of necessary action. Furthermore, it determines medical tasks as module practices and uses a unique coding system to describe incidents. This coding system has seven vectors for error classification: patient category, working shift, module practice, linkage chain (error type, direct threat, and indirect threat), medication, severity, and potential hazard. Such mathematical formulation permitted us to derive two parameters: error rates for module practices and weights for the aforementioned seven elements. The error rate of each module practice was calculated by dividing the annual number of incident reports of each module practice by the annual number of the corresponding module practice. The weight of a given element was calculated by the summation of incident report error rates for an element of interest. This model was applied specifically to nursing practices in six hospitals over a year; 5,339 incident reports with a total of 63,294,144 module practices conducted were analyzed. Quality assurance (QA) of our model was introduced by checking the records of quantities of practices and reproducibility of analysis of medical incident reports. For both items, QA guaranteed legitimacy of our model. Error rates for all module practices were approximately of the order 10-4 in all hospitals. Three major organizational factors were found to underlie medical errors: “violation of rules” with a weight of 826 × 10−4, “failure of labor management” with a weight of 661 × 10−4, and “defects in the standardization of nursing practices” with a weight of 495 × 10−4.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] To the Editor: We retrospectively reviewed published variants in the PKD1 genes and detected errors in 39 of 771 variants (5.06% (95% c.i., 3.62–6.85)). All arose from human processing mistakes. As peer-reviewed publication is no safeguard for those considering the clinical significance of ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 65 (1993), S. S235 
    ISSN: 1432-1246
    Keywords: Biomarker ; Dietary restriction ; Murine leukemia virus ; PCR ; Rate of aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined effects of aging on endogenous retrovirus gene expression of mouse lymphocytes with a hypothesis that it may be a useful biomarker of aging. Mice have endogenous murine leukemia viruses (MuLVs) in their chromosomes. We detected the gene expression of long terminal repeats (LTRs) of MuLVs. Brains, livers and spleens were taken from young (3 months old) and old (27 months old) male C57BL/6 mice. In addition to these control (C) mice, we also determined gene expression in dietary restricted (DR) mice, in which rates of aging are known to be slowed. RNA was extracted from the tissues and converted into cDNA. The MuLV-LTR portion of cDNA was amplified by polymerase chain reaction (PCR). The PCR products were analyzed by agarose gel electrophoresis. Gene expressions of young mice were found to be tissue-specific. Expressed LTRs from brains, livers and spleens were that of 370 base-pairs (bp), those of 370 and 620 bp, and those of 370, 400 and 620 bp, respectively. Old mice of C group, however, decreased tissue specificity: expressed LTRs became those of 370–400 by in any tissues. In contrast the tissue specific gene expression was conserved in old DR mice which had to get prolonged life span and decreased lymphoma incidence. Thereby, gene expression of endogenous retroviruses appears to change during aging and to be modifiable by life-prolonging DR. It may be therefore used as a biomaker of aging in mice. Humans are known to have similar gene elements like MuLV. The present findings demonstrate a possibility of application of endogenous gene expressions to the epidemiology of aging.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0738
    Keywords: OOS-TMP ; Hypothermia ; Fischer 344 rats ; Housing temperature ; Toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We explored the effects of O,O,S-trimethyl phosphorothioate (OOS-TMP) on body temperatures in Fischer 344 female rats. The 7-day LD50 p.o. for Fischer 344 female rats was found to be 11.8 mg/kg. OOS-TMP induced long-lasting (more than 48 h) and extensive hypothermia at doses 〉 14 mg/kg at a typical laboratory temperature (22° C) while it produced typical symptoms at 10 mg/kg without hypothermia. In contrast, pair-fed (to 20 mg/kg rats) rats (n=4) did not become hypothermic, negating any role of hypophagia in OOS-TMP associated hypothermia. We next investigated the effects of housing temperatures on toxicities at a LD50 dose (12 mg/kg). At 30° C (n=11) and 22° C (n=13), rats did not have hypothermic bouts but at 15° C, eight out of ten rats had. Evidence that changes of housing temperatures neither modified clinical symptoms nor changed mortality rates discards a possibility of hypothermia being involved in delayed toxicity. A novel result of the present study suggests that thermoregulation may be heavily impaired by a special class of organophosphorus compounds.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0738
    Keywords: Testicular toxicity ; Ethylene glycol monomethyl ether ; PGK-2 ; Sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phosphoglycerate kinase (PGK, EC 2.7.2.3), which is expressed specifically in sperm and spermatids, is an enzyme in the Embden-Meyerhof pathway that converts glucose to pyruvate. We developed an electrophoresis method to determine relative PGK-2 quantity and applied it to evaluate spermatogenesis activity. In the ethylene glycol monomethyl ether (EGME)-induced testicular toxicity, relative PGK-2 quantity had not decreased until 4 weeks of exposure. Mean relative PGK-2 quantities, defined as PGK-2 quantity over PGK-1 quantity in a pooled spleen sample (±SD) were: 1.43±0.32 for control animals (N=10); 1.67±0.24 for the group exposed at 500 mg/kg for 5 days (N=6); 1.85±0.58 for the group exposed at 500 mg/kg for 2 weeks (N=6); 0.09±0.06 for the group exposed at 500 mg/kg for 4 weeks (N=6); not detectable in animals exposed at 500 mg/kg for 5 weeks (N=7); 0.208±0.103 for the group exposed at 250 mg/kg for 5 weeks (N=6); and 1.35±0.38 for the group exposed at 125 mg/kg for 5 weeks (N=6). These relative quantities showed a good correlation with sperm/spermatid counts (r=0.823,p〈0.01) and histological findings. These findings suggest that EGME has toxicity on primary spermatocytes and spermatogonia. In the case of sterility associated with a chromosomal abnormality (chromosomal translocation between chromosome X and 16), relative PGK-2 quantity was not detected in any of the seven adult (12 weeks of age) mice, although many primary spermatocytes were detected by histological examination. Those findings suggest that cellular differentiation is arrested at meiosis due to the chromosomal abnormality. It was thus concluded that relative PGK-2 quantity provides information on testicular development and is therefore useful as an indicator of testicular function.
    Type of Medium: Electronic Resource
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