Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 2005-2009  (3)
  • 2000-2004  (13)
  • 1965-1969  (2)
  • 11
    Electronic Resource
    Electronic Resource
    Deposition and Characterization of Polycrystalline Silicon Thin-Films by Reactive Thermal Chemical Vapour Deposition at 450°C (2003)
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 93 (June 2003), p. 281-286 
    Details
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/26/transtech_doi~10.4028%252Fwww.scientific.net%252FSSP.93.281.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 90-96 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An engineered yeast with emission of fluorescence from the cell surface was constructed. Cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (GFP). A glucose-inducible promoter GAPDH as a model promoter was selected to control the expression of the reporter gene in response to environmental changes. The GFP gene was fused with the gene encoding the C-terminal half of α-agglutinin of Saccharomyces cerevisiae having a glycosylphosphatidylinositol anchor attachment signal sequence. A secretion signal sequence of the fungal glucoamylase precursor protein was connected to the N-terminal of GFP. This designed gene was integrated into the TRP1 locus of the chromosome of S. cerevisiae with homologous recombination. Fluorescence microscopy demonstrated that the transformant cells emitted green fluorescence derived from functionally expressed GFP involved in the fusion molecule. The surface display of GFP was further verified by immunofluorescence labeling with a polyclonal antibody (raised in rabbits) against GFP as the first antibody and Rhodamine Red-X-conjugated goat anti-rabbit IgG as the second antibody which cannot penetrate into the cell membrane. The display of GFP on the cell surface was confirmed using a confocal laser scanning microscope and by measuring fluorescence in each cell fraction obtained after the subcellular fractionation. As GFP was proved to be displayed as an active form on the cell surface, selection of promoters will endow yeast cells with abilities to respond to changes in environmental conditions, including nutrient concentrations in the media, through the emission of fluorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002539900307
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Localisation of biogenic amines in larvae of Bugula neritina (Bryozoa: Cheilostomatida) and their effects on settlement (2000)
    Springer
    Marine biology 136 (2000), S. 1-9 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Distributions of serotonin and catecholamines in larvae of the marine bryozoan Bugula neritina (Bryozoa: Cheilostomatida) were investigated using immunohistochemistry with anti-serotonin antiserum and glyoxylic acid–induced fluorescence histochemistry. Anti-serotonin immunoreactive substances and glyoxylic acid–induced fluorescent substances had similar distributions in the equatorial neuromuscular ring, the neural plexus, the paired axial neuromuscular cords, and tracts connecting the neural plexus to ciliated cells bordering the pyriform organ. The effects of dopamine, noradrenaline, adrenaline, tyramine, octopamine, synephrine and serotonin, at 10−4, 10−5 and 10−6  M, on settlement were analysed. In filtered seawater, 98% of larvae settled in 3 h, but only 11%, 3% and 6% total settlement was observed after 8 h in 10−4  M dopamine, 10−4  M serotonin and 10−5  M serotonin, respectively. Total settlement was 70% in 10−4  M noradrenaline, 80% in 10−4  M adrenaline and 60% in 10−4  M tyramine. Less than 60% settlement was observed in 10−4 and 10−5  M octopamine and synephrine. Serotonin's inhibitory effect on settlement was mimicked by a range of serotonin receptor agonists and antagonists, among which 5-carboxamidotryptamine was the most potent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050001
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Development of full-length Trk B-immunoreactive structures in the prefrontal and visual cortices of the macaque monkey (2000)
    Springer
    Anatomy and embryology 201 (2000), S. 139-147 
    Details
    ISSN: 1432-0568
    Keywords: Key words Frontal cortex ; Visual cortex ; Primate ; BDNF ; NT4/5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Distribution and morphological changes of cells containing the signal transducing neurotrophin receptor, full-length Trk B (fl-Trk B), were investigated in the prefrontal cortex (area FD) and the primary visual cortex (area OC) of the macaque monkey between embryonic day 140 and the adult stage. In area FD at the adult stage, fl-Trk B immunoreactivity was mainly observed in the pyramidal cells in layers II/III, V and VI. Small numbers of granule cells in layer IV were immunopositive. Bipolar and multipolar cells in layer II were rarely immunoreactive. At embryonic day 140, the number of fl-Trk B immunoreactive pyramidal cell was high, and gradually decreased until the adult stage. In layer IV, the number of fl-Trk B-ir cells was also high at embryonic day 140, and decreased remarkably from postnatal day 7 to the adult stage. On the other hand, in area OC at the adult stage, cells in layers II/III, IV, V and VI were fl-Trk B immunopositive. From embryonic day 140 until adulthood, the cells in layer IVc were fl-Trk B immunoreactive. The strongest fl-Trk B immunoreactivity in areas FD and OC occurred at postnatal month 6, coinciding with the time of the synapse overproduction. These findings suggest that ligands of fl-Trk B, such as BDNF and NT4/5 may be involved in the development and maintenance of the monkey cerebral cortices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008234
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Bright atomic beam by a temporal Zeeman acceleration (2000)
    Springer
    Applied physics 70 (2000), S. 561-565 
    Details
    ISSN: 1432-0649
    Keywords: PACS: 32.80.Pj
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. A novel method to produce a slow, monochromatic, and bright pulsed atomic beam from a magneto-optical trap by switching the magnetic field of the trap is proposed. A pulsed lithium atomic beam with a brightness of 1.1×1015 /sr s and a velocity of 13 m/s was produced as an experimental proof of this technique. The conversion efficiency from the trap into the atomic pulse was nearly 100%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003400050862
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    N-Methylation ability for azaheterocyclic amines is higher in Parkinson's disease: nicotinamide loading test (2000)
    Springer
    Journal of neural transmission 107 (2000), S. 985-995 
    Details
    ISSN: 1435-1463
    Keywords: Keywords: Parkinson's disease ; N-methylation ; nicotinamide ; aging.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. The discovery of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) leads to the hypothesis that Parkinson's disease (PD) is may be initiated or precipitated by endogenous toxins by the mechanism similar to that of MPTP in genetically-predisposed individuals. The higher cerebrospinal fluid levels of N-methylated azaheterocyclic amines, such as β-carboline and tetrahydroisoquinoline, have been found in parkinsonian patients compared with age-matched controls. To estimate the N-methylation ability for azaheterocyclic amines in parkinsonian patient, nicotinamide was dosed with 100 mg to 26 parkinsonians and 20 controls consisted of 16 other neurogenic disease patients and 4 healthy volunteers. The urine was collected for 4 h, and then analyzed urinary its metabolites by an improved HPLC method. Nicotinamide has a pyridine ring in its structure and may be metabolized through the pathways similar to those for the endogenous neurotoxins. The urinary excretions of nicotinamide metabolites were significantly affected by aging. The excretion of N1-methylnicotinamide decreased along with aging both in PD patients and controls. In younger (65 years old or younger) PD patients, the excretion amount of N1-methylnicotinamide was significantly higher than that in younger controls. The decline rate of N1-methylnicotinamide excretion in parkinsonians was significantly greater than that in controls; the rate is more than 2-fold higher in parkinsonian patients. The age-associated de-crease in 1-methyl-2-pyridone-5-carboxyamide excretion was observed only in parkinsonian patients, but not in controls. The total excreted amount of N-methylated metabolites (N1-methylnicotinamide plus 1-methyl-2-pyridone-5-carboxyamide) was also observed the age-related decline in both groups. The urinary excretions of nicotinamide and nicotinamide-N-oxide were not influenced by aging. These results would indicate that the excess N-methylation ability for azaheterocyclic amines before the onset had been implicated in PD. On the other hand, the present results suggested that the contribution of aberrant cytochrome P450 or aldehyde oxidase activity acting on the pyridine ring, that could act as detoxification routes of endogenous neurotoxins, would be small in the etiology of PD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007020070047
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Control of body fluid volume regarded as a feedback system (1966)
    Springer
    Medical & biological engineering & computing 4 (1966), S. 567-574 
    Details
    ISSN: 1741-0444
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire Pour simuler l'auto-régulation du volume de fluide du corps humain au moyen de la tonicité, des modèles sont mis au point de différentes manières et l'on prouve que tous permettent de reproduire la réponse type d'organismes quelconques à l'ingestion d'eau. La variété des modèles est dûe à des incertitudes sur la physiologie des reins et des hormones que ces modèles utilisent comme principe de départ.
    Abstract: Zusammenfassung Kontroll-Modelle des Körperflüssigkeitsvolumens durch Tonus sind auf verschiedene Weisen entwickelt worden, und alle zeigen, daß es möglich ist, die charakteristische Antwort des Organismus auf Aufnahme von Wasser zu reproduzieren. Die Verschiedenheit der Modelle ist auf Unsicherheiten der zugrundeliegenden Nieren-und Hormonphysiologie zurückzuführen.
    Notes: Abstract Control models of body fluid volume via tonicity are developed in several ways, and all are demonstrated to be able to reproduce the characteristic response of organisms to ingestion of water. The diversity of models is due to uncertainties in renal and hormonal physiology, on which they are based.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02474826
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Testicular dermoid cyst in an 85-year-old man (2000)
    Springer
    International journal of clinical oncology 5 (2000), S. 144-145 
    Details
    ISSN: 1437-7772
    Keywords: Key words Dermoid cyst ; Testis ; Prostate cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of dermoid cyst of the right testis. An 85-year-old man was admitted to our hospital with an enlarged right testicle and a high prostate specific antigen (PSA) level. A hard testis of approximately 10 cm, with a smooth surface, was palpated. On digital rectal examination, a hard prostate of approximately 4 cm, with irregular surface, was palpated. We performed right inguinal orchiectomy and transrectal ultrasound-guided prostate needle biopsy. The wall of the testis was calcified, with a cyst that contained yellow-white sebaceous material and tufts of hair. Microscopically, the cyst wall showed calcification and ossification, and had hair roots. The diagnosis was dermoid cyst. We also found a well differentiated adenocarcinoma (Gleason 2–3) in the prostate. Dermoid cyst of the testis is very rare. To our knowledge, this case is in the oldest patient thus far reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101470050107
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...