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  • 11
    ISSN: 1437-7799
    Keywords: Key words AST-120 ; Glomerulosclerosis ; Hyperlipidemia ; Renal failure ; SHC rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. The oral adsorbent, AST-120 Kureha Chemical, has been shown to attenuate the progression of chronic renal failure in rats and humans. Spontaneous hypercholesterolemic male rats, (SHC rats; SHCRs) have been introduced for experimentation because they develop progressive hyperlipidemia and glomerulosclerosis on a cholesterol-free standard diet by their 30th week of life. Methods. The effects of AST-120 were studied in SHCRs. Twenty 10-week-old SHCRs were divided into two groups: a control group (n = 10), and an AST-120 group (n = 10). The experiment was begun at the 12th week and completed at the 34th week of life. Results. At the end of the experiment, we found that the serum levels of total cholesterol were 40% lower in the AST-120 rats than in the control rats (P 〈 0.01). The creatinine clearance in the AST group was 40% higher than that in the controls (P 〈 0.05). At the age of 20 weeks, postheparin lipoprotein lipase in the AST-120 SHCRs and in Sprague-Dawley rats with normal serum lipid levels was comparable, but was clearly lower in the control SHCRs. Finally, in a pathological investigation that determined a sclerosis index for all kidneys, this was significantly lower in the AST group than in the control animals (P 〈 0.01). Conclusions. The reduction of serum lipid levels following the administration of the AST-120 oral adsorbent is associated with amelioration of renal functional and structural changes in SHCRs.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental nephrology 4 (2000), S. 156-159 
    ISSN: 1437-7799
    Keywords: Key words Human parvovirus B19 ; Hemolytic uremic syndrome ; Mesangiolytic glomerulonephritis ; Globotetraosyl ceramide (Gb4)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 59-year-old woman developed hemolytic uremic syndrome (HUS) associated with acute human parvovirus B19 (HPVB19) infection. A renal biopsy revealed glomerular mesangiolysis with segmental hypercellularity; mild fibrinogen/fibrin deposits were noted by immunofluorescence study and severe endothelial injury was noted electron microscopically. The histological findings were compatible with HUS. We discuss the relation of HPVB19 to HUS, with special reference to the tropism of the virus for endothelial cells.
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1432-0584
    Keywords: Keywords Immune thrombocytopenic purpura ; Intracranial hemorrhage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We sent questionnaires to hospitals in Japan in order to study the incidence and conditions of intracranial hemorrhage (ICH) in children with immune thrombocytopenic purpura (ITP). From 1980 to 1995, 11 cases of ICH were reported in eight patients with ITP at 35 institutions. One patient had ICH four times, but only one patient died of the condition. From 1990 through 1995, ICH occurred in four (0.52%) of 772 patients with ITP. None of the patients died. The platelet count when ICH occurred was 5.2±3.7×109/l (mean±SD) (n=11). Four of the eight patients (1980–1995) had received active treatment [e.g. intravenous immunoglobulin G (i.v. IgG)] immediately before ICH occurred. In seven cases (1980–1995), possible causes of ICH, including menstruation (n=2) and viral infections (n=3), were identified. Systemic lupus erythematosus (SLE) later developed in three patients. Although the incidence of ICH in children with ITP has not decreased compared with the rates in earlier studies, the mortality rate has decreased markedly. Our results suggest that menstruation, infection, and risk factors for progression to SLE may help to predict ICH in children with ITP. Large-scale prospective trials are needed to identify risk factors for ICH.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1420-908X
    Keywords: Key words: MCP-1 – Macrophage infiltration – Neutrophil infiltration – IL-8 – LPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective and Design: To evaluate the mechanism whereby monocyte chemoattractant protein (MCP)-1 attracts monocytes in vivo.¶Subjects: New Zealand white rabbits (175 rabbits) were used.¶Treatment: LPS, MCP-1 or IL-8 was injected into knee joints. Antibodies against various cytokines or IL-1 receptor antagonist were injected to neutralize cytokine activities.¶Methods: The numbers of leukocyte populations, levels of cytokines in joints were estimated.¶Results: Partial inhibition of neutrophil influx with anti-IL-8 IgG (10 μg) suppressed LPS-induced macrophage influx by 43 ± 8.5% (p〈0.05) without affecting the MCP-1 level. Intraarticular injection of MCP-1 (1-30 μg) induced macrophage influx. The event was accompanied by a small num-ber of neutrophils in an early phase. Co-injection of IL-8 (1.0 μg) enhanced the MCP-1-induced macrophage infiltration (p〈0.01). In neutrophil-deplete d rabbits, LPS failed to induce macrophage influx even though the MCP-1 level was maintained, and macrophage influx following exogenously administered MCP-1 was also dramatically inhibited.¶Conclusions: Early events associated with neutrophil infiltration appear to be important for MCP-1 to induce a later macrophage influx in LPS-arthritis.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    International journal of biometeorology 44 (2000), S. 24-30 
    ISSN: 1432-1254
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract  In this research, we hypothesized that, in rats, adaptation to high altitude (2500 m) plus training at low altitude (610 m), ”living high–training low”, improves physical performance at low altitude more than living and training at low altitude (610 m). Rats were divided into four groups: (1) living at low altitude (LL, n=12), (2) living and training at low altitude (LLTL, n=13), (3) living at high altitude (LH, n=12), (4) living at high altitude and training at low altitude (LHTL, n=13). The program for living at high altitude involved raising rats under hypobaric hypoxia (equivalent to 2500 m), and the training program consisted of running on a tread-mill at low altitude. All groups were raised at each altitude and trained to run at 35 m/min for 40 min/day, 6 days/week for 6 weeks. During this program, we measured heart rates both at rest and during exercise, and performed running-time trials. The mean heart rate during exercise was lower in groups with training than in groups without training, and the groups receiving training could run longer than the untrained groups. The LHTL group especially showed the lowest mean heart rate during exercise and the longest running time among all groups. After 6 weeks of the training program, all rats had a catheter implanted into the carotid artery, and the mean systemic arterial pressure was continuously measured during treadmill running. The rate of increase of this pressure as the running intensity increased was lower in groups with training than in groups without training, especially in the LHTL group. Finally, we anesthetized all the rats and extracted both the right and left ventricles, and the triceps surae and liver. Training increased the weight of the left ventricle, triceps surae, and liver. The increase in weight of the left ventricle and triceps surae was higher in the LHTL group than in the LLTL group in particular. It appeared that living high– training low may be an effective strategy to improve performance ability at low altitude.
    Type of Medium: Electronic Resource
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  • 16
    ISSN: 1432-1076
    Keywords: Infantile spasms ; Etiological factors ; Clinical aspects ; Long term prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The etiological factors, clinical aspects and long term prognosis were studied in 200 patients with infantile spasms. Forty-eight (24.0%) died and the rest were aged 6 years or more at the time of final follow-up. In 73 (36.5%) the etiology was prenatal, in 44 (22.0%) perinatal, and in 17 (8.5%) postnatal: 18 cases (9.0%) were cryptogenic. The remaining 48 (24.0%) patients were doubtful cases. The mortality of the pre-and perinatal cases at 35.6% and 34.1% respectively was significantly higher than that in the other etiologic groups (P〈0.001). With regard to the onset of spasms, these were very carly in the pre-and perinatal groups, whereas in the postnatal group the onset was late. The onset in the doubtful and cryptogenic groups was in between. Some 44.4% of the cryptogenic cases showed normal mental and physical development after the age of 6 years, whereas less than 10% of the prenatal and perinatal group did so. Almost all of the doubtful cases became mentally subnormal. The incidence of a family history of epilepsy or other convulsive disorders in the first, second and third degree relatives was highest in the cryptogenic cases (40.0%), and lowest in the perinatal cases (9.3%) (P〈0.01). The incidence of laughing attacks was highest in the postnatal cases (42.9%), against no such attacks in cryptogenic cases (P〈0.01). The attacks seemed to be linked with organic brain lesions. Among various factors related to the etiology of infantile spasms, genetic propensity seemed especially important in cryptogenic cases.
    Type of Medium: Electronic Resource
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  • 17
    ISSN: 1432-1076
    Keywords: EEG ; Evoked potentials ; Newborn ; Intracranial hemorrhage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The EEG, visual and auditory evoked potentials (VEP, AEP) were evaluated in 16 full-term newborn infants who had intracranial hemorrhage documented by computerized tomography (CT). Three of them had supratentorial, three, supra- and infratentorial hemorrhage, while the other ten had infra- or peri-tentorial bleeding. Three died during the neonatal period. Eight of the 13 surviving infants were neurologically normal and five were abnormal at the time of the follow-up. Those who had normal or mildly abnormal background EEGs all developed normally, while those whose neonatal EEG was severely abnormal subsequently developed neurological sequelae irrespective of the extent of intracranial hemorrhage. The EEG, VEP or AEP is of little value in the diagnosis of intracranial bleeding but the EEG is valuable in assessing the degree of associated parenchymatous damage and is of great prognostic significance.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Amino acids 14 (1998), S. 343-352 
    ISSN: 1438-2199
    Keywords: Amino acids ; HIV ; Entropy evolution rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We analyze the variation of HIV after infection by means of an information measure, called the entropy evolution rate. In our analysis, we use a part of the external glycoprotein gp120 including the V3 region observed from six patients. Then we could make the following two aspects clear; (1) the relation between the change of the entropy evolution rate and the appearance of symptoms of disease, and (2) the relation between the change of the entropy evolution rate and that of the CD4 count of the patients.
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 1432-1866
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract A supersaline, metal-rich brine (ca. 40 weight% total chloride salt) was extracted from 3708 m depth of well WD-1a in the Kakkonda Granite by reverse circulation after a standing time about 196 hours. The estimated borehole temperature exceeds 500 °C near the bottom. Tritium content and the relationship between δD and δ18O show that the injected borehole fluid (river water) evidently mixed with an isotopically heavy and ancient fluid. The phase analysis showed that a gas phase separated from a brine and that a brine concentrated in the borehole as the borehole temperature recovered after cooling by drilling fluids. We think the original fluid was trapped in the Kakkonda Granite and mixed with the borehole fluid through fine fractures induced by thermal stress.
    Type of Medium: Electronic Resource
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 261 (1999), S. 307-316 
    ISSN: 1617-4623
    Keywords: Key words Protein phosphatase-2C gene family ; Mesembryanthemum crystallinum ; Tissue specificity ; Environmental stress ; Transcript expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten transcripts (Mpc1-10) homologous to protein phosphatases of the 2C family have been isolated from the halophyte Mesembryanthemum crystallinum (common ice plant). Transcripts range in size from 1.6 to 2.6 kb, and encode proteins whose catalytic domains are between 24% and 62% identical to that of the Arabidopsis PP2C, ABI1. Transcript expression is tissue specific. Two isoforms are present only in roots (Mpc1 and Mpc5), three in young leaves (Mpc6, 8 and 9), two in old leaves (Mpc6 and Mpc8), and two in post-flowering leaves (Mpc8 and Mpc9). Mpc2 is strongly expressed in roots and also in seeds, meristematic tissues and mature flowers. Mpc3 is specific for leaf meristems, and Mpc4 is found in root and leaf meristems. Mpc7 is restricted to meristematic tissues. Mpc10 is only present in mature flowers. Mpc2 (in roots and leaves), Mpc5 (in roots) and Mpc8 (weakly in leaves) are induced by salinity stress and drought conditions with different kinetics in different tissues, but other Mpcs are downregulated by stress. Cold stress (4° C) leads to a decline in Mpc5 and Mpc6, but low temperature provoked a long-term (days) increase in Mpc2 levels in leaves and a transient increase (less than 24 h) in roots. Four full-length transcripts have been obtained. In each case, after over-expression in E.coli, the isolated proteins exhibited (Mg2+-dependent, okadeic acid-insensitive) protein phosphatase activity, although activity against 32P-phosphocasein varied among different PP2Cs. Determination of tissue developmental and stress response specificity of PP2C will facilitate functional studies of signal-transducing enzymes in this halophytic organism.
    Type of Medium: Electronic Resource
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