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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistological Double Labeling of Apolipoprotein E in Different Cell Types of the Human Atherosclerotic Plaque (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 598 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb42331.x
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  • 2
    Electronic Resource
    Electronic Resource
    73. Erfahrungen bei der orthotopen Xenotransplantation der Hundeleber auf das Schwein (1971)
    Springer
    Langenbeck's archives of surgery 329 (1971), S. 222-223 
    Details
    ISSN: 1435-2451
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurde bei 11 Schweinen eine orthotope Xenotransplantation der Hundeleber durchgeführt. Die Überlebensdauer war durchschnittlich 4,3 ± 1,5 Std. Die Todesursachen waren generalisierte Blutungen. Um die hyperakute Abstoßungsreaktion zu bremsen, wurden vor der Verpflanzung der Hundeleber bei 5 Fällen Hundeniere und Hundemilz je 20 min und bei 5 anderen Fällen eine zweite Hundeleber am Femoralkreislauf eingeschaltet. Wegen der stark aufgetretenen Kreislaufreaktion (Tachykardie und Hypotonie) war die Überlebensdauer noch kürzer. Aus gleichen Gründen wurden hohe Dosen Prednisolon intraund postoperativ bei 5 Fällen verabreicht. Die Überlebensdauer von 4 Schweinen war 9,0 :L 2,2 Std. Ein Schwein überlebte 5 Tage. Bei diesem Schwein wurde Hundeproteinsynthese beobachtet.
    Notes: Summary Orthotopic xeno-transplantations of dog livers into 11 pigs were carried out. The survival time was on average 4.3 ± 1.5 h. The cause of death was generalized haemorrhage. To inhibit the hyperacute rejection, before trans-planting the dog liver we connected dog kidneys and dog spleens in 5 cases and in another 5 cases a second dog liver to the femoral circulation, 20 min, before the transplant, in each case. In view of the very strong circulatory reaction (tachycardia and hypotension) the survival time was even shorter. For the same reason high doses of prednisolone were administered intra- and postoperatively in 5 cases. The survival time of 4 pigs was 9.0 ± 2.2 h. One pig survived for 5 days, and in this animal we were able to observe dog-protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01770490
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  • 3
    Electronic Resource
    Electronic Resource
    Der Effekt von hohen Dosen Prednisolon auf die hyperakute Abstoßungsreaktion bei der orthotopen Xenotransplantation der Hundeleber auf das Schwein (1972)
    Springer
    Research in experimental medicine 157 (1972), S. 107-122 
    Details
    ISSN: 1433-8580
    Keywords: Xenograft ; Livertransplantation ; Prednisolon ; Xenotransplantation ; Lebertransplantation ; Prednisolon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Es wurde in 11 Fällen die orthotope Xenotransplantation der Hundeleber auf das Schwein durchgeführt. Die Überlebensdauer betrug 4,3 ± 1,5 Std. Todesursache war eine generalisierte Blutung. 2. Um die hyperakute Abstoßungsreaktion zu bremsen, wurden bei 5 Fällen hohe Dosen Prednisolon verabreicht. Die Überlebensdauer von 4 Empfängern war 9 ± 2,2 Std, 1 Schwein überlebte 5 Tage. Die Bremsung der hyperakuten Abstoßungsreaktion ist durch hohe Dosen Prednisolon teilweise möglich.
    Notes: Summary 1. 11 orthotopic xenotransplantations of canine liver to pig were carried out. The survival time was 4.3 ± 1.5 hrs. The death occured because of general bleeding. 2. To stop the hyperacute rejection high dosage of prednisolon was given intraand postoperativ in 5 cases. 4 recipients survived 9 ± 2.2 hrs and 1 of them 5 days. It is partly possible to stop the hyperacute rejection of xenografts dog-to-pig with high dosage of prednisolon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851692
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  • 4
    Electronic Resource
    Electronic Resource
    Distribution pattern of matrix metalloproteinases 1, 2, 3, and 9, tissue inhibitors of matrix metalloproteinases 1 and 2, and α2-macroglobulin in cases of generalized AA- and AL amyloidosis (2000)
    Springer
    Virchows Archiv 437 (2000), S. 521-527 
    Details
    ISSN: 1432-2307
    Keywords: Amyloid Protease Protease inhibitors Matrix metalloproteinases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Matrix metalloproteinases (MMPs) degrade basement membranes and connective tissue and play an essential role in the homeostasis of the extracellular matrix which is disrupted by the deposition of amyloid. This immunohistochemical study investigated the distribution pattern of matrix metalloproteinases (MMP-1, -2, -3, and -9) and their inhibitors [α2-macroglobulin (α2-M), tissue inhibitors of MMPs (TIMP)-1, and TIMP-2] in human AA- and AL amyloid deposits. Specimens of liver, kidney, and spleen from 22 autopsy cases were investigated. Nine patients had suffered from generalized AA amyloidosis, eight from generalized AL amyloidosis, and five from rheumatoid arthritis or tuberculosis with no histological evidence of amyloid. In all amyloidotic and non-amyloidotic patients, each protease and protease inhibitor was detected in almost every organ investigated. In the amyloidotic cases, there was no indication that a specific protease or protease inhibitor was absent or expressed, but a difference was observed in their spatial distribution patterns. The most noticeable difference was found in immunostaining of amyloid. Only MMP-1, -2, and -3, and α2-M were present in AA amyloid deposits, and only TIMP-1 and TIMP-2 were found in deposits of AL amyloid. This is the first study to show that MMP-1, -2, and -3 are present in AA amyloid deposits. They may be involved in tissue remodeling or in proteolysis of the precursor and fibril proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280000271
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  • 5
    Electronic Resource
    Electronic Resource
    Inducible nitric oxide synthase expression in human urinary bladder cancer (2000)
    Springer
    Virchows Archiv 437 (2000), S. 662-666 
    Details
    ISSN: 1432-2307
    Keywords: Nitric oxide synthase Immunohistochemistry Transitional cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Nitric oxide (NO) is generated by a family of enzymes, nitric oxide synthases (NOS), in a wide range of mammalian cells. NO produced by the inducible NOS isoform (iNOS) has been suggested to play an important role in tumor biology with both tumor promoter and antitumor activity. Here, the cellular localization of iNOS in tissue of 100 cases of urinary bladder cancer was assessed immunohistologically using a commercially available antiserum. Positive iNOS immunostaining was detected in all samples of tumor tissue, whereas nonmalignant tissue adjacent to malignant areas did not show any iNOS positivity. The tumor tissue revealed a highly inhomogeneous staining pattern. In addition to uniformly stained tumor specimens, we also found markedly iNOS-positive tumor islets in the midst of unstained tumor tissue and scattered individual tumor cells expressing marked staining. In some cases, the tumor tissue showed no or only weak staining intensity. In some instances, the superficial epithelial layer of papillary carcinomas was extremely immunoreactive, in other cases it was not. Thus we were unable to show a clear correlation to tumor grade or stage. Further studies with a diversity of tumor markers including molecular genetics techniques will be necessary to elucidate how and to what extent NO and bladder cancer of different grades and stages are functionally interrelated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280000296
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  • 6
    Electronic Resource
    Electronic Resource
    Case report 669 (1991)
    Springer
    Skeletal radiology 20 (1991), S. 294-298 
    Details
    ISSN: 1432-2161
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02341670
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  • 7
    Electronic Resource
    Electronic Resource
    Is typing of metaplasia at the squamocolumnar junction revealing its aetiology? (2000)
    Springer
    Virchows Archiv 436 (2000), S. 6-11 
    Details
    ISSN: 1432-2307
    Keywords: Key words Intestinal metaplasia ; Squamocolumnar junction ; Gomori’s aldehyde fuchsin ; H. pylori ; Barrett’s mucosa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Until recently, intestinal metaplasia (IM) at the squamocolumnar junction (SCJ) was ascribed to Barrett’s mucosa (BM), which arises from gastro-oesophageal reflux. Recent studies, however, have shown that IM at the SCJ can also be induced, for example, by Helicobacter pylori (HP). The aim of this study was to investigate whether the type of IM might be helpful in the differentiation between these two aetiologies. Biopsies from the antrum, corpus and immediately below the Z-line were taken from 443 patients. Eighty-three of them showed IM below the Z-line. In these, the endoscopic aspect of the Z-line was classified as either unremarkable (n=49) or suspected of BM (n=34). Typing of IM was done using Gomori’s aldehyde fuchsin–Alcian blue staining. Overall, age, HP status and erosive oesophagitis had no influence on the IM type. Type-III IM (n=24) was more frequent in men (P=0.0371) and related to endoscopic BM (P〈0.0001). Type-I/II IM (n=59) was associated with an unremarkable Z-line (P〈0.0001) and was linked to multifocal gastric IM (P=0.016) and HP (P=0.0011). In conclusion, it was shown that, in the presence of a normal Z-line, especially in the absence of HP, type-III IM is suggestive of BM. The diagnosis of short or ultra-short segment BM should therefore include endoscopic, histological and histochemical characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008200
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  • 8
    Electronic Resource
    Electronic Resource
    Plasmacytoma of the tonsil with AL amyloidosis: evidence of post-fibrillogenic proteolysis of the fibril protein (2000)
    Springer
    Virchows Archiv 436 (2000), S. 336-344 
    Details
    ISSN: 1432-2307
    Keywords: Key words Amyloid ; λ Light chain ; Osseous metaplasia ; Plasmacytoma ; Protease ; Tonsil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We report of a 58-year-old Caucasian man who was referred to the University Hospital with a greatly enlarged left tonsil which showed calcifications on computed-tomography scans. Histopathology revealed a plasmacytoma with secondary AL amyloidosis, ossifications, and multinucleated foreign-body-type giant cells. N-terminal sequencing of amyloid-fibril proteins purified from the formalin-fixed tissue showed the presence of two proteins of different size; these were of λ-light-chain origin (subgroup V), measured approximately 15.2 kDa and 10.5 kDa, and had identical N-terminal ends (YVLTQPP). When the amyloid deposits were immunolabeled with a polyclonal antibody directed against λ light chain, they showed two staining patterns: some deposits showed intense immunolabeling while others were not immunoreactive. Immunostaining of amyloid was completely absent after protease pre-treatment. Immunoelectron microscopy with gold-labeled secondary antibodies showed staining that was spatially related to amyloid fibrils and suggested that the antibody probably detected the fibril protein. Therefore, our hypothesis in this case is that the different immunostaining patterns are due to a post-fibrillogenic proteolysis of the fibril protein at the C-terminal end of the light chain, as indicated by the presence of two differently sized λ-light-chain fragments with identical N-terminal ends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050456
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  • 9
    Electronic Resource
    Electronic Resource
    Chondroblastoma of bone (1992)
    Springer
    Virchows Archiv 421 (1992), S. 355-366 
    Details
    ISSN: 1432-2307
    Keywords: Chondroblastoma ; Bone tumours ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The clinical and morphological findings of 53 chondroblastomas in the files of the Bone Tumour Registry of Westphalia are presented. The mean age of all patients was 19.2 years. The male-to-female ratio was 1.5∶1. Forty-two of the tumours (79.8%) were located in the long tubular bones and short tubular bones of the hands and were closely related to the growth plate. Six cases (11.3%) were found in the flat bones, 4 cases (7.5%) in the tarsal bones and 1 case (1.9%) in the craniofacial bones. The characteristic radiological feature of 44 investigated lesions was a mostly eccentric radiolucency with a geographic pattern of bone destruction and matrix calcifications. Periosteal reaction was evident in 9% of the cases. Most tumours demonstrate the typical morphological features of chondroblastoma, but 3 cases resembled a giant cell tumour. In 2 cases a haemangio-pericytomalike growth pattern was observed. Nine of the tumours had an aneurysmal bone cyst-like component. Vascular invasion was seen in 1 case. Immunohistochemically most cells in 30 of the cases and fetal chondroblasts in 3 cases were strongly positive with vimentin and S-100 protein. Collagen type II was positive in the chondroid matrix of the tumours and in fetal cartilage tissue; collagen type VI was present focally around individual tumour cells and was always seen in the chondroid matrix of the lesions and in fetal cartilage. These findings support the cartilaginous nature of these tumours. In paraffin sections, 46.6% of the cases revealed a distinct positive reaction of some tumour cells with the monoclonal cytokeratin antibody KL1 (molecular weight 55–57 kDa). Only 4 of them demonstrated a coexpression with the other monoclonal cytokeratin anti-body CK (clone MNF 116, molecular weight 45–56.5 kDa). In paraffin sections all fetal chondroblasts were negative with both cytokeratin antibodies. Frozen sections of 3 tumours showed a strong positive reaction with both cytokeratin antibodies in many chondroblasts, indicating an “aberrant” cytokeratin expression. Osteoclast-like giant cells stained positive with leucocyte-common antigen (LCA) and with the macrophage-associated antibody KP1, but were negative with the other macrophage-associated antibody MAC 387. Recurrence rate was 10.7%. The clinical course of all tumours was benign.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01660984
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  • 10
    Electronic Resource
    Electronic Resource
    Mutation of p53 with loss of heterozygosity in the osteosarcomatous component of a dedifferentiated chondrosarcoma (2000)
    Springer
    Virchows Archiv 436 (2000), S. 494-497 
    Details
    ISSN: 1432-2307
    Keywords: Key words Dedifferentiated chondrosarcoma ; p53 mutation ; p53 LOH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We investigated a dedifferentiated chondrosarcoma of a 61-year-old woman with an osteosarcomatous high-grade component for p53 alteration. The low-grade cartilaginous and the high-grade osteosarcomatous components of the tumor were macrodissected and evaluated separately by immunohistochemistry and molecular biology. We used PCR-SSCP analysis and direct sequencing to screen exons 4–8 for p53 mutations. The p53 intron 1-polymorphism was investigated for loss of heterozygosity. A functionally relevant p53 missense mutation in codon 193 of exon 6 (A-to-T transversion) with loss of wild-type allele was detected only in the dedifferentiated component. Using the monoclonal antibody DO-1, immunohistochemistry failed to show p53 overexpression. This evidence of p53 mutation may be regarded as at least a co-factor that ”switched” the preexisting low-grade conventional chondrosarcoma to a highly malignant dedifferentiated tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050478
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