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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Neurochemical research 25 (2000), S. 341-347 
    ISSN: 1573-6903
    Keywords: Neuronal survival ; apoptosis ; Heat-Shock Proteins ; HSP-70 ; NMDA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cerebellar granule cells (CGC) die apoptotically after five days in culture (DIV) at physiological concentrations of potassium (5 mM; K5). When CGC are depolarized (K25) or treated with NMDA (150 μM) cell survival is increased. CGC changed from K25 to K5 die after 24–48 h. It is known that heat shock protein (HSP) may protect from cell death. Here, we found that cells in K5 showed an increase in HSP-70 levels after 3 DIV. Similarly, in cells changed from K25 to K5, HSP-70 levels were increased after 6 h. Neither NMDA nor K25 treatment affected HSP-70 levels from 2–7 DIV. Ethanol or thermal stress induced HSP-70, but cell survival was not affected in K5 medium. These results suggest that HSP, particularly HSP-70, are not involved in the mechanisms by which NMDA and KCl promote cell survival.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 86 (2000), S. 318-323 
    ISSN: 1432-1955
    Keywords: Key wordsCryptosporidium ; Endemicity ; Primates ; Herbivores ; Barcelona zoo (Spain)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The prevalence of fecal shedding of Cryptosporidium in 36 primates (21 species and subspecies) and 62 herbivores (36 species and subspecies) housed at the Barcelona zoo was studied. Cryptosporidial oocysts were found in stool samples of 14 Primate, 18 Artiodactyla, 2 Perissodactyla, and 1 Proboscidea species. None of them showed symptoms related to the parasite. Neither the sex nor the group condition (alone or in a group) of the animals studied appeared to be correlated with parasitic prevalence. The results extend the host species range of the protozoan to 18 new animals (6 Primate, 10 Artiodactyla, 1 Perissodactyla, and 1 Proboscidea species) and confirm the endemic status of Cryptosporidium at the Barcelona zoo. We conclude that maintenance of the parasitic endemic status is probably due to the presence of animal carriers as well as to the physical features of some facilities where oocysts could remain viable and infectious.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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