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  • 2000-2004  (4)
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Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Identification of genes that are up-regulated in concert during sugar depletion in Arabidopsis (2004)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 27 (2004), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In order to identify sugar-responsive genes, Arabidopsis cDNA macro-arrays were hybridized with probes prepared from seedlings cultured in the presence or absence of sucrose. Initial screening identified 36 cDNAs that were affected, among which 11 were finally selected as being up-regulated under sugar-depleted conditions. Eight of them were novel in terms of known sugar responses. Based on the properties of encoded proteins, they were classified into three groups involved in amino acid metabolism, carbohydrate metabolism and unidentified nature. Subsequently, correlations between their expression profiles and sugar levels were analysed using intact plants. When detached leaves were subjected to sucrose depletion for 12 h, a marked decrease in glucose and sucrose occurred, and concomitantly all 11 genes were up-regulated. However, their transcripts rapidly diminished within 7 h when samples were re-supplied with sucrose. A similar pattern of up- and down-regulation was observed upon dark treatment of whole plants but not during natural senescence of mature leaves. Inhibition analyses with glucose analogues indicated that eight of these genes might be associated with the hexokinase signalling pathway. The present observations suggest that plants activate a set of genes involved in recycling of materials during sugar depletion, and that these genes are simultaneously and concertedly regulated despite functional diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2004.01146.x
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  • 2
    Electronic Resource
    Electronic Resource
    Differential expression of phospholipase D1 in the developing retina (2002)
    Oxford, UK : Blackwell Science, Ltd
    European journal of neuroscience 15 (2002), S. 0 
    Details
    ISSN: 1460-9568
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Expression patterns of phospholipase D1 (PLD1) in the developing rat retina were investigated using immunocytochemistry and Western blot analysis and compared with the expression patterns of glutamine synthetase. PLD1 immunoreactivity appeared first in a few neuroblasts in the middle of the mantle zone of the primitive retina by embryonic (E) day 13. PLD1-immunoreactive primitive ganglion cells were characterized in the ganglion cell layer by E17. Faint immunoreactivity at E17 profiled radially orientated cells and this pattern appeared up to postnatal (P) day 7. In the ganglion cell layer at P3, displaced amacrine cells and ganglion cells were classified. At P5, presumptive horizontal cells and amacrine cells were identified. By P7, a thin outermost layer of newly formed segments of the photoreceptor cells was also PLD1 immunoreactive. PLD1 immunoreactivity at P8 was limited to radial Müller cells and the outer segment layer of the photoreceptor cells, and the expression pattern was conserved to adulthood. Western blot analysis showed relatively high amounts of PLD1 protein at E17 and P3, a decrease at P7, and moderate amounts from P8 onward. Co-expression of PLD1 with glutamine synthetase in the retina appeared first after birth in differentiating neurons and in Müller cells by P8; thereafter the pattern was maintained. The expression pattern of the PLD1 during development of the retina suggests that PLD1 plays important roles in glutamate-associated differentiation of both specific neurons and radial glial cells, and in glutamate-mediated cellular signalling in Müller cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9568.2002.01939.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation and properties of ferroelectric BaTiO3 thin films produced by the polymeric precursor method (2000)
    Springer
    Journal of materials science 19 (2000), S. 1457-1459 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011027624446
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    High dielectric constant of SrTiO3 thin films prepared by chemical process (2000)
    Springer
    Journal of materials science 35 (2000), S. 4783-4787 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract SrTiO3 thin films were prepared by the polymeric precursor method and deposited by spin-coating onto Pt/Ti/SiO2/Si(100) substrates. The spin-coated films heat treated at 700°C were crack-free, dense, and homogeneous. Microstructural and morphological evaluations were followed by grazing incident X-ray, scanning electron microscopy and atomic force microscopy. Dielectric studies indicated a dielectric constant of about 475, which is higher than that of ceramic SrTiO3, and a factor dissipation of about 0.050 at 100 kHz. SrTiO3 thin films were found to have paraelectric properties with C-V characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004816611050
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    Paper (German National Licenses)
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