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  • 1
    Electronic Resource
    Electronic Resource
    Effects of human lung fibroblasts on eosinophil degranulation* (2000)
    Copenhagen : Munksgaard International Publishers
    Allergy 55 (2000), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Although eosinophils and fibroblasts are thought to contribute to the mechanisms of chronic asthmatic inflammation, the interaction between eosinophils and fibroblasts has not been thoroughly clarified. We examined eosinophil cationic protein (ECP) release from human eosinophils cultured in the presence of human lung fibroblast HFL-1. Methods: Eosinophils from healthy donors were cultured with or without C5a for 16 h in the presence of human fetal lung fibroblasts which had previously been incubated with or without TNF for 4 h. ECP in supernatants was measured by RIA. Results: ECP release was potentiated only when both eosinophils and fibroblasts were activated by C5a and TNF, respectively, while it was not significantly potentiated when either eosinophils or fibroblasts were activated. Paraformaldehyde fixation of fibroblasts had some suppressive effect on ECP enhancement, and mAb against GM-CSF partly inhibited ECP enhancement. Coculture of eosinophils and fibroblasts with stimulus treatment resulted in the enhancement of both eosinophil adhesion and ECP release. The potentiation of ECP release was partially inhibited by anti-ICAM-1 mAb, anti-CD18 mAb, and anti-CD29 mAb, which caused partial and comparable inhibition of the enhancement of eosinophil adhesion. Conclusions: This study suggests that the activation of fibroblasts may have some role in the potentiation of ECP release from cocultured eosinophils, and that adhesion of eosinophils to fibroblasts may partly be involved in the mechanism of ECP potentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2000.00623.x
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  • 2
    Electronic Resource
    Electronic Resource
    Increased amyloid β protein in the skin of patients with amyotrophic lateral sclerosis (2000)
    Springer
    Journal of neurology 247 (2000), S. 633-635 
    Details
    ISSN: 1432-1459
    Keywords: Key words Amyloid β protein ; Amyotrophic lateral sclerosis ; Skin ; Alzheimer's disease ; Enzyme-linked immunosorbet assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Distinct vascular and periadnexal immunoreactivity have been observed for amyloid b protein (Aβ) in skin from patients with amyotrophic lateral sclerosis (ALS). We used an enzyme-linked immunosorbent assay to make a more quantitative comparison of Aβ concentrations between ALS patients and controls. The insoluble fractions of skin samples from ALS patients contained significantly higher Aβ concentrations per milligram protein than those from controls. Various alterations in extracellular matrix components have been reported to occur in the skin of patients with ALS, and several matrix constituents have been shown to affect processing and aggregation of Aβ in human brain. Taking these previous findings together with those of the present study, our observations suggest that changes in extracellular matrix in skin of ALS patients may facilitate aggregation and deposition of Aβ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004150070133
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  • 3
    Electronic Resource
    Electronic Resource
    Cell-free protein synthesis in a microfabricated reactor (2000)
    Springer
    Bioprocess engineering 22 (2000), S. 13-17 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Microbiochemical reactors having two inlet ports and one outlet port were fabricated on a silicon wafer by means of anisotropic etching in order to develop a parallel and automatic experimental system for cell-free translation. Using cell-free extract prepared from Escherichia coli, we tested the reactor for the translation of polyuridylic acid and MS2 phage RNA, and found that polypeptide and protein syntheses could be proceeded according to the genetic codes on the mRNAs. It indicates that the microfabricated reactor is useful for enzymatic reactions including complicated ones like cell-free translation. We also discuss the possibility of microsystems as advanced experimental tools for not only cell-free translation but also other various biochemical and biological research fields.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00009093
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  • 4
    Electronic Resource
    Electronic Resource
    Antibacterial characteristics of magnesium oxide powder (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 187-194 
    Details
    ISSN: 1573-0972
    Keywords: Active oxygen ; antibacterial activity ; ceramic ; magnesium oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The antibacterial activity of magnesium oxide (MgO) was studied. Inhibitory zones appeared around the MgO powder slurry put directly on nutrient agar plates seeded with Escherichia coli or Staphylococcus aureus. However, no zone was observed using a penicillin cup to avoid contact between the bacteria and the MgO powder. Moreover, the supernatant solution of the MgO powder slurry and a MgCl2 solution containing Mg2+ at a concentration of the solubility of MgO did not affect the growth of E. coli and S. aureus. Moreover, elevated shaking speed increased the death of E. coli in the slurry, indicating that the contact frequency between bacterial cells and MgO powders affected the antibacterial activity. It was considered that the contact between MgO powder and bacteria was important for the occurrence of its antibacterial activity. Since the generation of active oxygen, such as O2 −, from the MgO powder slurry was detected by chemiluminescence analysis, an investigation was carried out to determine whether active oxygen generated from MgO powder slurry was related to its antibacterial activity. The changes in the antibiotic sensitivity in E. coli treated by MgO powder agreed with those by active oxygen treatment. These results suggested that the active oxygen generated from the MgO powder slurry was one of the primary factors in its antibacterial activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008916209784
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