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  • 1
    Electronic Resource
    Electronic Resource
    Cloning of the Human Puromycin-Sensitive Aminopeptidase and Evidence for Expression in Neurons (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 68 (1997), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The puromycin-sensitive aminopeptidase (PSA) is thought to contribute to the degradation of enkephalins. Besides being the most abundant aminopeptidase in the brain, PSA is expressed in other organs as well. From a human fetal brain cDNA library, we have isolated cDNA encoding the human PSA (huPSA) protein. The isolated cDNA gave rise to a protein with a molecular mass of 99 kDa. Compared with mouse PSA, homology at the amino acid and cDNA level was 98 and 93%, respectively. Translation of the huPSA was found to be initiated at the second of two possible start codons, as shown by studies with antibodies directed against peptide sequences of both potential N-terminal regions. Northern blot analysis with RNA isolated from different human organs demonstrated that the huPSA transcript is strongest but not exclusively expressed in the brain. Vesicular stomatitis virus epitope-tagged huPSA protein was expressed in HeLa cells and found to be localized in the cytoplasm, especially in the perinuclear region. By in situ hybridization, huPSA transcript could be identified in cortical and cerebellar neurons, whereas glial cells and blood vessels remained negative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1997.68030889.x
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  • 2
    Electronic Resource
    Electronic Resource
    Carbohydrate Recognition on MUC1-Expressing Targets Enhances Cytotoxicity of a T Cell Subpopulation (1997)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 46 (1997), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The influence of the epithelial mucin MUC1 on T cell-mediated lysis was analysed using lymph node lymphocytes (LNL) from patients with colorectal carcinoma. LNL were stimulated with allogeneic, MUC1-transfected B cells and the bulk cultures were cloned. Alloreactive cytotoxic T cell clones were obtained which preferentially lysed MUC1-expressing targets. The majority was CD4+ and MHC-class II-restricted, and a minor group was CD8+ and MHC-class I-restricted. All the clones expressed CD3 and TCRαβ, and were CD56−. The capacity to preferentially kill MUC1-expressing targets was stable in several clones for up to 6 months in culture. The enhancing effect of MUC1 on the lysis was investigated in more detail. It was only seen after inhibition of O-linked glycosylation in the targets. Furthermore, this effect was completely abrogated by the monoclonal antibody 3C9, directed against the Thomsen–Friedenreich antigen (T-antigen, Galβ1–3GalNAc bound α1–3 to Ser/Thr) as well as by the soluble disaccharide Galβ1–3GalNAc, but not by other similar disaccharides. The authors conclude that in their system the preferential killing of MUC1-expressing targets is due to the recognition of an internal carbohydrate epitope accessible on underglycosylated MUC1, possibly T-antigen, by an auxiliary receptor molecule on T cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.1996.d01-91.x
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  • 3
    Electronic Resource
    Electronic Resource
    Tumorous manifestation of hairy cell leukemia after long-term treatment with interferon alpha (1995)
    Springer
    Annals of hematology 70 (1995), S. 103-105 
    Details
    ISSN: 1432-0584
    Keywords: Hairy cell leukemia ; Interferon alpha Tumorous manifestation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Tumorous-manifestation of hairy cell leukemia in a patient treated with IFNα for 7 years is described. After this time, while the patient still was in hematological remission, a tumorous involvement of the lung by hairy cells developed and was successfully treated by surgery. No differences in the phenotype of hairy cells in the lung tumor, in the bone marrow, or in the blood could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01834389
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  • 4
    Electronic Resource
    Electronic Resource
    Tumorous manifestation of hairy cell leukemia after long-term treatment with interferon alpha (1995)
    Springer
    Annals of hematology 70 (1995), S. 103-105 
    Details
    ISSN: 1432-0584
    Keywords: Key words Hairy cell leukemia ; Interferon alpha ; Tumorous manifestation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Tumorous-manifestation of hairy cell leukemia in a patient treated with IFNα for 7 years is described. After this time, while the patient still was in hematological remission, a tumorous involvement of the lung by hairy cells developed and was successfully treated by surgery. No differences in the phenotype of hairy cells in the lung tumor, in the bone marrow, or in the blood could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01834389
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  • 5
    Electronic Resource
    Electronic Resource
    Primäre Hirnlymphome bei erworbenem Immundefektsyndrom Immunphänotypische und molekularpathologische Charakterisierung in der stereotaktischen Biopsie, Autopsie und Liquorzytologie (1995)
    Springer
    Der Pathologe 16 (1995), S. 75-80 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Aids ; Primäre Hirnlymphome ; Epstein-Barr Virus ; Immunglobulinschwerketten-Genumlagerung ; Key words AIDS ; Primary malignant lymphoma of the brain ; Epstein-Barr virus ; Ig heavy-chain rearrangements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In this study we present morphological, cytological, immunophenotypical, and molecular genetic features of ten cases of AIDS-related primary brain lymphomas obtained as sterotactic biopsies, autopsy specimens, or cerebrospinal fluid samples. Histologically, a very characteristic perivascular and diffuse lymphomatous spread in the brain parenchyma was observed. By applying a highly sensitive in situ hybridization technique (ISH) using digoxigenin-labeled anti-sense riboprobes, abundant Epstein-Barr virus (EBV)-encoded small nuclear RNAs (EBER) transcripts could be demonstrated in each case studied. A combination of ISH with immunohistochemical staining for latent membrane protein (LMP-1) and EBV nuclear antigen-2 (EBNA-2) showed different patterns of EBV latency with a predominance of the broad spectrum (EBER+/EBNA-2+/LMP-1+). Clonal rearrangements of immunoglobulin heavy chain (IgH) genes were demonstrated in four cases using a sensitive polymerase chain reaction. In one patient the rearrangement pattern suggested biclonal lymphoproliferation. Our observations confirm the implication of the EBV in the etiology of AIDS-related primary malignant lymphomas of the brain. The demonstration of EBV gene products and clonal IgH rearrangements even in small biopsy samples is a useful tool for distinguishing between reactive and neoplastic lymphocytic infiltrates, which are frequently observed in brain and cerebrospinal fluid specimens obtained from AIDS patients.
    Notes: Zusammenfassung In der vorliegenden Untersuchung stellen wir typische morphologische, zytologische, immunphänotypische und molekulargenetische Merkmale von 10 Fällen Aids-assoziierter Hirnlymphome vor, die als stereotaktische Biopsien, Autopsiematerial oder Liquorpunktate vorlagen. Mit einer hochsensitiven In-situ-Hybridisierungstechnik (ISH) unter Verwendung von digoxigeninmarkierten Antisensesonden konnten sehr zahlreiche Epstein-Barr-Virus(EBV-)kodierte kurze nukleäre RNA(EBER)-Transkripte in jedem der untersuchten Fälle dargestellt werden. Eine Kombination der ISH mit immunhistochemischen Färbungen zum Nachweis des latenten Membranproteins (LMP-1) und des EBV-Kernantigens-2 (EBNA-2) zeigte unterschiedliche Muster der EBV-Latenz mit Prädominanz des sog. „breiten Spektrums“ (EBER+/EBNA-2+/LMP-1+). Mit einer sensitiven Polymerasekettenreaktion (PCR) konnten klonale Immunglobulinschwerketten (IgH)-Genumlagerungen in 4 Fällen nachgeweisen werden. Bei einem Patienten war eine biklonale Lymphoproliferation nicht auszuschließen. Unsere Beobachtungen unterstreichen die Bedeutung des EBV bei der Entstehung primärer Aids-assoziierter Hirnlymphome. Der Nachweis von EBV-Genprodukten und die Klonalitätsanalyse mit Hilfe der PCR können einen wichtigen Beitrag zur differentialdiagnostischen Abgrenzung von reaktiven entzündlichen gegenüber neoplastischen lymphoiden Infiltraten leisten, die bei Aids-Patienten häufig im Hirngewebe und Liquor beobachtet werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050079
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  • 6
    Electronic Resource
    Electronic Resource
    Primäre pulmonale B-Zell-Lymphome vom MALT-Typ (1995)
    Springer
    Der Pathologe 16 (1995), S. 328-335 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Bronchus-assoziiertes lymphatisches Gewebe ; Marginalzonen ; B-Zell-Lymphome ; Reaktive Keimzentren ; Follikelkolonisation ; Lymphoepitheliale Läsionen ; Key words Bronchus-associated lymphoid tissue ; Marginalzone B-cell lymphoma ; Reactive germinal centers ; Follicular colonisation ; Lymphoepithelial lesions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The malignant B-cell lymphoma of the MALT-type shows significant differences from nodal lymphomas with respect to its biological, molecular genetic, and clinical properties. According to the proposal from the International Lymphoma Study Group for a Revised European-American Classification of Lymphoid Neoplasms, MALT-B-cell lymphomas have been defined as an extranodal subgroup of marginal zone B-cell lymphomas and may have lower and higher grade types. We have applied this classification to 24 cases of primary B-cell lymphomas of the lung. These tumors showed histopathologic features of low (n = 20) and/or high grade neoplasms (n = 4) recapitulating the structure of the acquired bronchus- associated lymphoid tissue (BALT). According to our experience, the diagnosis of BALT-type B-cell lymphoma, is both practically feasable and reproducible not only for surgical material but even for biopsy specimens.
    Notes: Zusammenfassung Maligne B-Zell-Lymphome vom MALT-Typ unterscheiden sich signifikant von nodalen Lymphomen bzgl. ihrer biologischen, molekulargenetischen und klinischen Eigenschaften. Nach den Vorschlägen der Internationalen Lymphom-Studiengruppe für eine revidierte europäisch-amerikanische Klassifikation lymphatischer Neoplasien sind MALT-B-Zell-Lymphome als extranodale Form von Marginalzonen-B-Zell-Lymphomen definiert, die einen niedrigeren oder höheren Malignitätsgrad aufweisen können. Wir wandten dieses Klassifikationskonzept auf 24 Fälle primärer pulmonaler B-Zell-Lymphome an. Alle Fälle zeigten histologische Merkmale niedrigmaligner (n = 20) und/oder hochmaligner Neoplasien (n = 4), die die Struktur des erworbenen bronchusassoziierten lymphatischen Gewebes (BALT) rekapitulierten. Nach unserer Erfahrung, ist die Diagnose von B-Zell-Lymphomen des BALT-Typs nicht allein an chirurgischen Resektaten, sondern auch an Biopsien in der praktischen Diagnostik möglich und reproduzierbar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050110
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  • 7
    Electronic Resource
    Electronic Resource
    Fucosyltransferase III and sialyl-Lex expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue (1996)
    Springer
    Glycoconjugate journal 13 (1996), S. 727-733 
    Details
    ISSN: 1573-4986
    Keywords: colon carcinoma associated mucins ; sialyl-Lex ; fucosyltransferases ; AM-3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The potential contribution of fucosyltransferases to the overexpression of sialyl-Lex antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Lex. This modulation of sialyl-Lex was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Lex, the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Lex moiety in HT-29 cells but not in human colon carcinoma tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702336
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