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  • 1990-1994  (2)
  • 1985-1989  (1)
  • 1
    ISSN: 1432-0584
    Keywords: Erythropoietin ; Tumor cells ; Growth regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recombinant human (rh) erythropoietin (EPO) is attracting increasing interest as an agent for treating cancer-related anemia. Thus, we have tested the effects of rhEPO on the clonal growth of 22 different cell lines derived from a wide range of human solid tumors (head and neck 3, lung 2, breast 2, stomach 1, colorectal 3, hepatocellular 1, pancreas 1, ovary 1, choriocarcinoma 1, osteogenic sarcoma 1, glioblastoma 2, neuroblastoma 1, prostate 1, renal 2) in vitro. RhEPO (dose range 0.01–100 U/ml) caused no significant and reproducible stimulation of clonal growth as measured by a capillary modification of the human tumor cloning assay in agar in any of the cell lines tested. In particular, there was no sensitivity for rhEPO of those cell lines which were shown to be responsive to interleukin-3 and GM-CSF. On the other hand, there were no growth inhibitory effects of rhEPO on the cell lines of this study. Finally, neutralizing anti-human EPO antibody had no effect on the clonal growth of two kidney carcinoma cell lines, making autocrine growth regulation by hEPO in these lines unlikely.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Sozial- und Präventivmedizin 30 (1985), S. 103-106 
    ISSN: 1420-911X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Immunity against tetanus in an urban population: gaps of immunity among elderly persons. In a sero-epidemiological survey we investigated the immunity against tetanustoxoid in an urban population. Children and adolescents birn in Germany (n=193) showed an excellent immunity, negative findings being the exception. Among soldiers of the army (n=136), negative findings were rare too. In these boths groups the antibody titiers averaged 4,5 IU/ml. It is generally agreed that an antibody titer of 0,01 IU/ml protects against tetanus. Adults born in Germany (n=295) had a good immunity: 84 % showed antibody — titers against tetanus. Among elderly persons, how ever, above 60 years of age (n=1576) there was a striking incidence of negative findings: in 1043 persons (65 %) no antibodies could be dewtected. The remaining 533 persons with positive findings (35 %) had antibody-titers in the lower range (average 0,482 IU/ml). Also among foreigners and immigrant labourers from mediterrean countries (Greece, Turkey, Yugoslavia) the frequency of negative findings was hishg: 183 persons were examined, 70 (39 %)were negative 113 (61 %) were positive. The incidence of negative findings was highest among women from the mediterrean countries. Several small studies published in Switzerland show a similar tendency. Our paper will encourage further seroepidemiological studies.
    Abstract: Resumé Au cours d'une étude sére-epidémiologique on a examiné l'immunité de la population d'une grande ville à l'égard du toxoide tétanique. Les enfants et les adolescents nés en République fédérale (n=193) étaient presque tous vaccinés; parmi les soldats de l'armée fédérale ne so sont trouvés presque que des résultats positifs également (n=136). Les titres d'anticorps de ces deux groupes étaient en moyenne de 4,5 Ul/ml (un titre d' anticorps d'au moins 0,01 Ul/ml protège contre le tétanos). Les enfants étrangers (nés en Yougoslavie.Crèce et Turquie (n=110), jouissaient d'une bonne protection contre la maladie. Parmi des Allemands adultes (n=295), les résultats positifs prédominaient (84 %). On a constaté un mauvais état de vaccination chez les personnes âgées de plus de 60 ans (n=1576): 533résultats positifs (35 %) contre 1043 résultats négatifs (65 %). Les traivailleurs étrangers en provenance de pays méditeranéens (n=183) étaient aussi insuffisamment vaccinés (39% de résultats négatifs), la situation étant moins bonne encore chez les femmes que chez les hommes. La situation en Suisse devrait être comparable comme le montrent quelches études. De telles enquêtes séro-épidémiologiques devraient être encouragées.
    Notes: Zusammenfassung In einer sero-epidemiologischen Studie wurde die Immunität einer Großstadtbevölkerung gegenüber Tetanustoxoid geprüft. Kinder und Jugendliche mit Geburtsort in der Bundesrepublik (n=193) waren fast lückenlos durchgeimpft; auch bei Soldaten der Bundeswehr waren fast nur positive Befunde zu finden (n=136). Die Antikörper dieser beidenGruppen lagen imDurchschnitt bei 4,5 IE/ml (ein Antikörpertiter von mindestens 0,01 IE/ml schützt gegen Tetanus). Bei ausländischen Kindern (Geburtsorte in Jugoslawien, Griechenland, Türkei; n=110) war der Impfschutz gut. Unter deutschen Erwachsenen (n=295) überwogen positive Befunde (84 %). Erhebliche Impflükken fanden sich bei älteren Menschen über 60 Jahren (n=1576): 533 (35 %) positive Befunde standen 1043 (65%) negativen Werten gegenüber. Auch ausländische Gastarbeiter aus dem Mittelmeerraum (n=183) wiesen Impflücken auf (39% negative Befunde), wobei Frauen Häufiger negativ waren als Münner. Die Verhältnisse in der Schweiz dürften ähnlich liegen, wie einige Studien erkennen lassen. Die Arbeit will zu sero-epidemiologischen Untersuchungen anregen.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Basic research in cardiology 85 (1990), S. 217-226 
    ISSN: 1435-1803
    Keywords: platelet activating factor ; cardiovascularshock ; ventricular contractilefailure ; coronaryvasospasm ; respiratorydistress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The platelet activating factor (PAF), a low molecular phospholipid, plays an important role in inflammation, anaphylaxis, and shock state development. In the isolated perfused guinea pig heart, PAF induces a decrease in coronary flow and cardiac contractility and atrioventricular conduction disturbances. Furthermore, PAF mediates a powerful bronchoconstrictory action causing a severe impairment in respiratory function. In the present study an attempt was made to separate cardiac from respiratory events during PAF-induced shock in vivo. PAF was injected intravenously (0.1–10 μg/kg) into anesthetized guinea pigs ventilated with room air or 100% oxygen. Administration of 10 μg/kg PAF was uniformly lethal: already within 2 min, cardiac output decreased by 60% and end-diastolic left ventricular pressure increased markedly indicating cardiac failure. ECG recordings showed signs of acute myocardial ischemia. Arrhythmias occurred in terms of atrioventricular conduction delay. Blood pressure initially increased, then declined continuously to below baseline within 10 min. All animals died within 25 min. Ventilation with room air was paralleled by development of severe hypoxia. However, under ventilation with 100% oxygen a dissociation between PAF-mediated cardiac and respiratory effects occurred. It is concluded that the PAF-induced shock is primarily based on direct cardiac damage. Furthermore, the ECG signs of ischemia are most likely due to coronary spasms.
    Type of Medium: Electronic Resource
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