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  • 1990-1994  (4)
  • 1980-1984  (2)
  • 1965-1969  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The effect of prostaglandins on the lysosomal function in the cervix uteri (1983)
    Springer
    Archives of gynecology and obstetrics 234 (1983), S. 1-16 
    Details
    ISSN: 1432-0711
    Keywords: Prostaglandin E2 ; Cervix uteri ; Lysosomes ; Human pregnancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Local or systemic prostaglandin (PG) administration leads to the known softening and dilatation of the cervix uteri. Lysosomal enzymes are involved in connective tissue degradation. The question arises whether the effect of PG on the cervix uteri is mediated by lysosomes. Five pregnant women (volunteers after informed consent) in the first trimester received 500 μg of PGE2-derivative (Nalador) i.m. at 12 and 8 h before termination by curettage. Five pregnant women without PG-treatment served as controls. Small biopsies were obtained from the endocervical canal and were immediately immersed in cold 2.5% glutaraldehyde and after further preparations examined under a Zeiss electron microscope 9S-2. A second portion of tissue was sliced and prepared for histochemical analysis of the acid phosphatase on lysosomes. Examination of the ultrastructure of the cervix uteri showed vesicles in the extracellular matrix. These were surrounded by a single membrane and contained either fine granular material of myelin-like whorls of membranes. These vesicles lay between collagen fibers, showed the reaction product of acid phosphatase and were often surrounded by an electron-lucent halo. We conclude that these matrix vesicles were “matrix lysosomes” extruded from the cervical myo-fibrocytes into the extracellular space as a result of the PG-E2-administration. Here they are not under cellular control and can initiate the proteolytic degradation of connective tissue. This might be the crucial step in cervical dilatation which, on ultrastructural examination, can be seen as decreasing electron density of the extracellular ground substance near the matrix lysosomes. The relationship between PGE2 and collagenase production is generally accepted. If one believes that lysosomal cathepsin D and cathepsin B act synergistically with collagenase, it can be assumed that PGE2 is involved in a lysosomal degradation of the connective tissue. The morphological sign of this occurrence is the release of matrix lysosomes by PGE2 as described in the present study. Extracellular lysosomes and their physiological significance in cervical function are discussed in detail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02114718
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  • 2
    Electronic Resource
    Electronic Resource
    Selective syncytium formation by murine leukemia virus in rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene (1990)
    Springer
    Archives of virology 115 (1990), S. 123-126 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 3Y1 rat fibroblasts transformed by adenovirus type 12 or by its E1A gene formed syncytia when cocultivated with the Friend as well as other ecotropic murine leukemia-virus-producing cells. In contrast, the parental 3Y1 cells and those transformed by other tumor viruses or by a chemical carcinogen exhibited no syncytium formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310628
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  • 3
    Electronic Resource
    Electronic Resource
    Selective planting of cationized, haptentized ovalbumin on the rat tubular basement membrane (1994)
    Springer
    Virchows Archiv 424 (1994), S. 587-591 
    Details
    ISSN: 1432-2307
    Keywords: Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195771
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  • 4
    Electronic Resource
    Electronic Resource
    Selective planting of cationized, haptenized ovalbumin on the rat tubular basement membrane (1994)
    Springer
    Virchows Archiv 424 (1994), S. 587-591 
    Details
    ISSN: 1432-2307
    Keywords: Tubular basement membrane ; Peritubular capillary ; Cationic antigen ; Ovalbumin ; Trinitrophenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We developed an experimental protocol for planting exogenous antigens with different molecular weights and charges on the constituents of the renal tubulointerstitium. The cationized antigens were injected selectively into the left renal arteries of Wistar rats. Antigen localization was documented by immunohistochemistry on frozen sections. Cationized bovine serum albumin (BSA; 68 kDa, isoelectric point =9.5) localized almost exclusively along the glomerular capillary wall. After application of highly cationic polyethyleneimine, cationized BSA given subsequently was found in a linear distribution along the glomerular capillary wall and along the peritubular capillaries. The fate of highly cationized ovalbumin conjugated with trinitrophenol (TNP-OA), subjected to gel filtration to obtain monomers (42 kDa, isoelectric point 〉10) differed; it was deposited in a linear pattern on the tubular basement membrane (TBM) and Bowman's capsule, and remained up to 36 h after injection. Noncationized, monomeric TNP-OA (42 kDa, isolectnic point =4.6) showed fine granular deposition in the tubular epithelium exclusively. These findings indicate that the barrier of the glomerular BM acts selectively on antigens with different molecular weights. They either settle on the peritubular capillaries, after passing the glomerular, or reach the urinary space, after which they are reabsorbed by the tubular epithelial cells to reach the TBM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01069737
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  • 5
    Electronic Resource
    Electronic Resource
    Suppression of murine leukemia virus-mediated 3Y1 cell fusion by expression of mouse MHC class I (1991)
    Springer
    Archives of virology 119 (1991), S. 43-52 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene formed syncytia by cocultivation with Friend murine leukemia virus (MuLV)-producing cells. On the other hand, parental 3Y1 cells and those derivatives induced by other tumor viruses or chemical carcinogen showed no MuLV-mediated syncytium formation [N. Momozaki et al. (1990) Arch. Virol. 115: 123–126]. The expression of major histocompatibility complex (MHC) class I mRNA and antigens was significantly reduced in these Ad12- and E1A-transformed 3Y1 cells. In contrast, other tumor virus- and chemical carcinogen-transformed 3Y1 cells expressed MHC class I almost in normal levels as did parental 3Y1 cells. Furthermore, Ad12-transformed 3Y1 cells which started to express the transfected exogenous MHC class I gene,H-2L d, showed no more MuLV-mediated 3Y1 cell fusion. These results indicate that the expression of MHC class I on the cell membrane is closely related to the inhibition of 3Y1 cell fusion by MuLV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314322
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  • 6
    Electronic Resource
    Electronic Resource
    Allgemeine, konstitutionelle und organische Aspekte schizophrener Mimik (1967)
    Springer
    European archives of psychiatry and clinical neuroscience 209 (1967), S. 450-461 
    Details
    ISSN: 1433-8491
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Von insgesamt 246 psychotischen Kranken wurden unter anderem auch 149 Schizophrene kinematographisch auf ihre mimischen Reaktionen hin getestet, zum Teil ist der Verlauf mit erfaßt. Die Besonderheiten der klinischen Gruppierungen mit deren jeweiliger Relation zu den Kretschmerschen Typen sind eingangs hervorgehoben. Leptosome und Dysplastiker traten zum Teil in „Kümmerformen“ auf; reine Pyknische waren seltener als Übergänge zum Dysplastischen. In 10% der Fälle ergaben sich abnorm symmetrische Gesichter von archaisch-infantiler Wirkung, in 20% auffällige Asymmetrien. — Hyperkinesen wie Grimassen, Faxen, Paramimien treten episodisch auf, es bleiben schließlich nur die Stereotypien, die sich vornehmlich in oberen Gesichtsbereichen abspielen; Ähnlichkeiten zu subcorticalen Hyperkinesen oder zum akinetischen Symptomenkomplex ergeben sich nicht; auch zu anderen Psychosen besteht mimisch keine Verwandschaft, bei exogenen Reaktionstypen ist allerdings eine Abgrenzung aus dem Zustandsbild allein nicht immer möglich, — Im Verlauf zerfällt die Mimik Schizophrener, wobei Ausgangstyp, Intensität und Dauer eine Rolle spielen; am ungünstigsten verhalten sich dabei „einfache Verläufe“ an Dysplastikern. Die Veränderung beginnt meist oral mit Innervationsunsicherheit der Lippen; in sieben Fällen war dabei ein halbseitiges Überwiegen nach Art einer „mimischen Parese“ festzustellen. Die Züge können völlig erschlaffen, was jedoch Ausnahmen sind; meist bleibt eine Überspannung in frontoorbitalen Bereichen zurück, wie man es ähnlich bei hirnatrophischen Prozessen findet; im Unterschied zu letzteren sind die atonischen unteren Gesichtsbereiche bei Schizophrenen jedoch rein motorisch intakt, dafür zeigt sich hier aber ein ungraziöses, seelenloses mimisches Verhalten, was Verfasser als thalamisches Syndrom irreversibler Natur deuten möchte.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01643455
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  • 7
    Electronic Resource
    Electronic Resource
    Haemorrhagic tumour necrosis following endotoxin administration (1984)
    Springer
    Virchows Archiv 403 (1984), S. 377-389 
    Details
    ISSN: 1432-2307
    Keywords: Meth A mouse tumour ; Endotoxin ; Haemorrhagic tumour necrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Endotoxin induced necrosis of the Meth A mouse tumour has been investigated using macroscopic, histological and ultrastructural examination methods. On the 8th day after tumour cell transplantation, the animals received a relatively non-toxic dose of the Salmonella abortus equi endotoxin intravenously. The natural history of the tumour necrosis took the following course: 1. The earliest morphological changes could be seen with the electron microscope 90 min after administration of the endotoxin, and were seen as an interstitial oedema with separation of the tumour cells. 2. Haemmorrhagic necrosis of the tumour was complete 4 hours after injection, and could be easily recognized with the naked eye. 3. Rejection of the necrotic malignant tumour was complete two weeks after LPS administration. Only minor residual scarring of the belly-wall remained. Haemorrhagic tumour necrosis due to endotoxin can be compared with the localized Shwartzman reaction and probably involves tumour necrotizing factor (TNF). For complete destruction of a tumour by haemorrhagic necrosis the size of the tumour is critical. Certain regression after endotoxin administration depends upon additional T-cell-mediated immunity (provided the tumour is immunogenic). In contrast to the haemorrhagic necrosis, BCG-induced tumour regression is accompanied by granulomatous inflammation, which may be responsible for destruction of the tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737287
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