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  • 1985-1989  (2)
  • 1980-1984  (1)
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  • 1985-1989  (2)
  • 1980-1984  (1)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Isolation procedure for bovine retinal rod outer segments (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 894-895 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An isolation procedure to obtain rod outer segments from cattle retinas is reported. Centrifugation of homogenates in discontinuous and continuous sucrose density gradients yields purified photoreceptor cell outer segments. Assay of the final preparation for rhodopsin content gives a ratio of 2.4 for DO280 nm/DO498 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01953777
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Binding of 11-cis retinaldehyde to the partially purified cellular retinaldehyde binding protein from bovine retinal pigment epithelium (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 582-586 
    Details
    ISSN: 1420-9071
    Keywords: CRALBP ; 11-cis retinaldehyde ; retinal pigment epithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 11-cis retinaldehyde binding analysis was performed on a bovine retinal pigment epithelium preparation of cellular retinaldehyde binding protein (CRALBP), whose purity degree was estimated as 75%. Equilibrium binding studies were carried out measuring the replacement of tritium-labeled with unlabeled 11-cis retinaldehyde at 25°C. Analysis of the experimental data both by a direct curve-fitting procedure utilizing a non linear least square regression analysis and by a conventional Scatchard plot revealed a single non-interacting binding site with an apparent equilibrium constant of 0.9×10−7 M. A binding stoichiometry of approximately 1 mol of 11-cis retinaldehyde/mol of binding protein can be calculated from the experimental data. Competition studies carried out in the presence of unlabeled ‘trans’ and ‘cis’ isomers of Vitamin A derivatives confirm the high degree of specificity of the 11-cis retinaldehyde binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02143594
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of a soluble lipid-protein complex carrying endogenous 11-cis retinaldehyde from bovine retinal pigment epithelium (1989)
    Springer
    Molecular and cellular biochemistry 85 (1989), S. 181-189 
    Details
    ISSN: 1573-4919
    Keywords: 11-cis retinaldehyde ; retinal pigment epithelium ; shed disc digestion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary A soluble lipid-protein complex in bovine retinal pigment epithelium is shown to carry endogenous 11-cis retinaldehyde, in the extent of 15% of the total 11-cis retinaldehyde found in this tissue. The complex, analyzed with respect to its chemical composition, exhibits a lipid composition close resembling the lipid composition of the rod outer segment membrane; the SDS-PAGE evidences the presence of a number of protein bands, two of which of 34 and 27 kDa appear glycoproteins. Finally, the lipid-protein complex exhibits a discrete level of a Cathepsin D-like protease activity. From the above, the possibility is discussed that the soluble lipid-protein complex could represent some phagolysosomal inclusion occurring in the pigment epithelial cells upon rod outer segment phagocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00577113
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    Paper (German National Licenses)
    Fulltext
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