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  • 1985-1989  (1)
  • 1950-1954  (3)
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  • 1
    ISSN: 1432-0584
    Keywords: Hairy cell leukemia ; Immunohistochemistry ; Interferon ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Bone marrow biopsies from 7 patients afflicted with hairy cell leukemia were studied with a panel of monoclonal antibodies at different intervals during alpha recombinant interferon therapy. Monoclonal antibodies Dako-LC and F 8.11.13 were used because they are highly reactive with hairy cells, and 82 H 3 and LeuM 1 were also utilized to identify respectively the residual hemopoietic and myeloid tissue. All antibodies are reactive on sections of formalin-fixed, paraffinembedded biopsy material. Before therapy a uniform hairy cell infiltrate was present and very little tissue was visible even after immunohistochemical staining. During therapy, identification of hairy cells in routine biopsies was difficult, since they were mixed with normal cells and the bone marrow was generally hypoplastic. Immunohistochemical stains seem to allow better identification of hairy cells and a more precise estimate of the degree of repopulation by normal bone marrow cells. It was evident, especially after immunocytochemical analysis, that interferon drastically reduced the extent of the infiltrate and allowed recovery of normal hemopoiesis, but did not produce complete remissions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 7 (1951), S. 138-140 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Riassunto Sono stati isolati i cromosomi da globuli bianchi umani normali e leucemici. Le osservazioni compiute su materiale colorato a fresco e con la reazione di Feulgen hanno permesso di constatare: 1° La possibilità di riconoscere cromosomi isolati anche nella specie umana; 2° Un diverso comportamento dei cromosomi normali rispetto ai leucemici anche nella fase di riposo cellulare concordamente a quanto osservato sulle mitosi.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 8 (1952), S. 345-347 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Riassunto Gli Autori hanno compiuto l'analisi cromatografica degli idrolisati di nuclei, cromosomi isolati ed istone di cellule ematiche normali e patologiche. Vennero esaminati: eritrociti di pollo; leucociti (normali e da ascesso) di cavallo; leucociti umani normali; linfociti di leucemia linfatica e leucociti di leucemia mieloide. I vari componenti cellulari ottenuti mediante il Waring blender e successive centrifugazioni differenziali; vennero idrolizzati con HCL. Con gli idrolisati vennero allestiti cromatogrammi bidimensionali in due sistemi di solventi; butanolo acetico e fenolo, fenolo e collidinalutidina. In tutti i cromatogrammi bidimensionali dei due sistemi di solventi si sono potuti mettere in evidenza 14 amino acidi (acido aspartico, acido glutammico, serina, glicina, treonina, alanina, prolina, valina, tirosina, leucina, fenil-alanina, arginina, lisina e istidina).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Chromosoma 4 (1950), S. 621-629 
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. A comparison has been made between the chromatic threads isolated from erythrocyte nuclei and chromosomes of diploid metaphase plates of several vertebrates. A close resemblance in morphological and structural features was found. It is therefore concluded that isolated filaments are true resting stage chromosomes. 2. Measurements showed all species to possess rather similar ranges of length of isolated chromosomes, but different species differ characteristically in the frequencies of the different length groups. The mitotic chromosomes, measured on published drawings, correspond closely in length with the isolated chromosomes within a species and show the same interspecific differences.
    Type of Medium: Electronic Resource
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