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  • 1985-1989  (2)
  • 25.55  (1)
  • 97.10  (1)
  • Arachis  (1)
  • 13.25.+m
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  • 1
    ISSN: 1432-2048
    Keywords: Arachis ; Cell culture ; Enzyme induction (cell culture) ; Phenylalanine ammonia-lyase ; Phytoalexin ; Stilbene synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A peanut (Arachis hypogaea L.) cell-suspension culture susceptible to selective induction of stilbene formation was established. The principles of defense responses of the whole plant were found to be retained in the artificial system. The suspension culture was characterized by its growth curve and by various biochemical parameters. In the stationary phase, reached 8 d after transfer to a new medium, the formation of stilbenes and stilbene synthase could be induced without altering the levels of other enzymes. Eighteen hours after applying an artificial elicitor (ultraviolet-C light) or 4 h after eliciting with a crude preparation of Phytophthora cambivora cell walls, phenylalanineammonia-lyase activity was increased eightfold and stilbene-synthase activity 20-fold. The activity of phenylalanine ammonia-lyase reached its peak at a slightly different time from that of stilbene synthase. The main products of L-phenylalanine metabolism in the induced cells were resveratrol, 3,3′,5-trihydroxy-4′-methoxystilbene and isopentenylresveratrol. Likewise, feruloyl-CoA reductase, as a parameter of lignin formation, was enhanced following induction, albeit with a different time course and with a less steep increase than found for phenylalanine ammonia-lyase and stilbene synthase.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 334 (1989), S. 491-510 
    ISSN: 1434-601X
    Keywords: 25.55 ; 97.10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The22Ne(α, γ)26Mg and22Ne(α, n)25Mg reactions were investigated forE α(lab) from 0.71 to 2.25 MeV. Neon gas enriched to 99% in22Ne was recirculated in a differentially pumped gas target system of the extended type. Theγ-ray transitions were observed with Ge(Li) detectors and the neutrons with3He ionization chambers. A previously known resonance at ER(lab)=2.05 MeV was verified and 15 new resonances were found in the energy range covered, with the lowest at ER(lab)=0.83 MeV. Information on resonance energies, widths, strengths,γ-ray branching ratios, as well asJ π assignments, is reported. The energy range investigated corresponds to the important temperature range ofT 9 from 0.3 to 1.4 (109 K), for which the astrophysical rates were determined for both reactions. The results show that the ratios of the rates for22Ne(α, n)25Mg and22Ne(α, γ)26Mg are significantly smaller than the previously adopted values, e.g., by at least a factor of 60 nearT 9=0.65. Thus, the22Ne(α, n)25Mg reaction will likely play a smaller role as a neutron source fors-process nucleosynthesis, than has frequently been assumed.
    Type of Medium: Electronic Resource
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