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  • 1
    ISSN: 1432-1335
    Keywords: Monoclonal antibodies ; Flow cytometry ; Carcinomas ; Surface antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Peripheral blood mononuclear cells (PBMC) from 40 patients with gastrointestinal carcinoma (GIC), 13 patients with primary carcinoma in other localizations(non-GIC), and from 57 apparently healthy donors were isolated by Ficoll-Paque gradient centrifugation. The separated cells were stained with several monoclonal antibodies and subjected to analysis on a fluorescence-activated cell sorter. A decreased percentage of PBMC expressing T cell antigens was noted amongst GIC patients, and was mainly due to a reduction of the Leu 2a subset, thus, leading to an increase in the Leu3a/Leu2a ratio from 1.4 to 2.1. Non-GIC patients had decreased numbers of both T helper and suppressor cells. Amongst PBMC from GIC and non-GIC patients a statistically increased percentage of cells expressed LeuM2 (P〈0.001), LeuM3 (P〈0.001), OKM 1 (P〈0.005), VEP 9 (P〈0.001), and HLA-DR (P〈0.001) antigens compared to healthy controls. The percentage of cells bearing these monocyte/macrophage antigens correlated well with the number of cells having monocyte morphology, stained for non-specific esterase, phagocytosed latex particles, and expressed Fc IgG receptor. Our results demonstrate clearly that tumor-bearing patients have an incrased relative number of monocytes. The data suggest that cells of the macrophage lineage may be involved in defense mechanisms and changes of the immune system evoked by various tumors.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-2568
    Keywords: chronic idiopathic intestinal pseudoobstruction ; neuronal degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Neuropathological examination of the gut in a patient with chronic idiopathic intestinal pseudoobstruction and temporal lobe epilepsy showed a degeneration of Auerbach's and of Meissner's plexus. The extent of degenerative changes increased in an aboral to oral direction. Neuronal degeneration was characterized by ballooning of the cytoplasm of the ganglial cells, by a hyperargyrophilia, a shortening and dilation of cell processes, and a progressive fragmentation and loss of axoris. In rectal biopsies, a PAS-positive granular material was detected in the cytoplasm of ballooned ganglial cells. A severe peripheral neuropathy with fiber degeneration in the posterior columns and axonal lesions within ventral nerve roots and a more recent fiber degeneration in the lateral columns corresponding to the picture of subacute combined degeneration of the spinal cord and a symmetrical Ammon's horn sclerosis was present. This case of progressive intestinal neuronal degeneration of unknown origin is a newly described condition leading to chronic idiopathic intestinal pseudoobstruction.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei insgesamt 120 Patienten wurden im Rahmen routinemäßiger gastroduodenoskopischer Untersuchungen Schleimhautbiopsien zur Kultivierung vonCampylobacter pyloridis entnommen. Bei sechs von sieben Patienten mit Ulcus ventriculi, 14 von 15 mit Ulcus duodeni, 15 von 20 mit Erosionen der Magenschleimhaut, 31 von 61 mit Gastritis und fünf von fünf mit Duodenitis konnteC. pyloridis isoliert werden. Hingegen war der Keimnachweis bei allen 12 Patienten mit histologisch unauffälliger Mukosa nicht möglich. Bei chronisch aktiver Gastritis ließ sichC. pyloridis signifikant häufiger als bei chronisch inaktiver Gastritis nachweisen (100 bzw. 50%). Diese Ergebnisse stützen die Hypothese vonMarshall undWarren, die besagt, daßC. pyloridis möglicherweise eine wichtige Rolle in der Entstehung von Gastritis und Ulcus pepticum zukommt.
    Notes: Summary During routine gastroduodenoscopic examination of 120 patients, biopsies of gastric mucosa were taken for the isolation ofCampylobacter pyloridis. The organism was isolated from six of seven patients with ulcus ventriculi, 14 of 15 with ulcus duodeni, 15 of 20 with erosions of the gastric mucosa, 31 of 61 with gastritis and five of five with duodenitis. In contrast, the cultures were negative in all of the 12 patients with histologically normal mucosa.C. pyloridis was isolated significantly more frequently from patients with active chronic gastritis than from those with inactive chronic gastritis (100% vs. 50%). These results support the suggestion ofMarshall andWarren that this bacterium may play an important role in the development of gastritis and peptic ulcer.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 34 (1989), S. 1094-1099 
    ISSN: 1573-2568
    Keywords: metabolic bone disease ; 25-hydroxyvitamin D ; Crohn's disease ; bone mineral density ; cortical area ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Vitamin D deficiency is frequently observed in patients with Crohn's disease and may be associated with an increased risk of development of metabolic bone disease. To estimate the incidence of metabolic bone disease by noninvasive methods, 31 patients (17–75 years old) with Crohn's disease and low 25-hydroxy vitamin D (25-OHD) levels in winter were investigated in the following summer by measuring the bone mineral content (BMC) of the distal radius by single photon absorptiometry and the cortical area ratio (CAR) calculated from radiographs of the right hand and by x-ray of the lumbar spine. Forty-five percent of the patients showed signs of metabolic bone disease. BMC and CAR correlated with 25-OHD serum levels (P〈0.05), especially in men. Furthermore, the amount of sun exposure has an influence not only on 25-OHD serum levels both in summer and in winter (P=0.0006), but also on the BMC (P=0.07). Consequently, vitamin D deficiency is of major importance for the development of metabolic bone disease in patients with Crohn's disease. Vitamin D deficiency can be prevented by increasing sun exposure and long-term vitamin D supplementation.
    Type of Medium: Electronic Resource
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