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  • 1
    Electronic Resource
    Electronic Resource
    European multicentre validation trial of two new non-invasive tests for the detection of  Helicobacter pylori antibodies: urine-based ELISA and rapid urine test (2003)
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 18 (2003), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim : Non-invasive tests for the assessment of Helicobacter pylori status are now an integral part of the management strategies for patients with dyspepsia. The aim of this study was to evaluate a urine based antibody ELISA and a near patient urine test for the diagnosis of H. pylori infection in a European population.Methods : Urine samples were collected from 449 patients (240 females, 209 males, mean age 54 years), with dyspeptic symptoms but no previous H. pylori eradication therapy, at five centres in four European countries. All patients underwent GI endoscopy and biopsies were taken for H. pylori diagnosis. Urine samples were analysed using an IgG ELISA (URINELISA) and a near patient urine test (RAPIRUN). In addition, a serum IgG ELISA (Pyloriset-EIA-GIII), a whole blood test (Pyloriset-Screen) and a 13C-urea breath test were performed.Results : The sensitivity of the urine based ELISA and the near patient urine test was 90% and 82%, and the specificity 68% and 83%, respectively. The accuracy of the serum ELISA and the whole blood test was comparable with the urine based test.Conclusion : The urine based ELISA and the near patient urine test are just as accurate as the serological tests. This comparable accuracy and complete non-invasiveness of the former gives it an advantage over blood based tests. This limits the application of these tests in general practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2003.01761.x
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  • 2
    Electronic Resource
    Electronic Resource
    The prevalence of ureaplasma, mycoplasma and antibodies against chlamydia group antigen in gynecological outpatients (1985)
    Springer
    Archives of gynecology and obstetrics 236 (1985), S. 203-209 
    Details
    ISSN: 1432-0711
    Keywords: Prevalence ; Ureaplasma ; Mycoplasma ; Chlamydia ; Gynecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 177 women with an average age of 30 years were investigated for ureaplasma, mycoplasma and for antibodies against the chlamydia group antigen. Specimen of endocervical mucus and catheter specimens of urine were cultured and the ELISA (“enzyme-linked immunosorbent assay”) technique applied. In addition the purity class (I to III) of gram-stained vaginal smears was determined. Ureaplasma were isolated from 45.8 and 26.0%, mycoplasma from 9.0 and 7.9% of cervical and urine specimens respectively. The patients were divided into 5 clinical groups. Patients in the first three groups (I–III, inflammatory disorders, bleeding anomalies, benign and malignant neoplasms of the genital tract) showed a significantly (p ≤ 0.05) higher colony count of ureaplasma in their urine than patients in the other two groups (IV and V, who had attended the hospital for reproductive problems or for routine examination). There was a clear correlation between the isolation rates of ureaplasma and mycoplasma and the purity classes of the vaginal smears. The rate of isolation increased progressively with class II and III smears. Antibodies against the chlamydia group antigen were detected in about 40% of all sera tested. A very high titer of antibodies reflecting a recent chlamydial infection was found in 11% of the sera tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133937
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  • 3
    Electronic Resource
    Electronic Resource
    Polymerase chain reaction for detection ofChlamydia pneumoniae in gargled-water specimens of children (1995)
    Springer
    European journal of clinical microbiology & infectious diseases 14 (1995), S. 141-144 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The objective of the present study was to establish the occurrence ofChlamydia pneumoniae by direct detection in gargled-water specimens obtained from 193 children suffering from acute or chronic respiratory infections. Specimens were analyzed by an indirect immunofluorescence test (IIF), a genus-specific antigen enzyme immunosorbent assay (EIA) and the polymerase chain reaction (PCR). The pathogen was detected in three children by PCR only. As underlying disease, chronic obstructive bronchitis resistant to therapy was reported. In two of the children, the presence of pneumonia could be verified by X-ray. With a detection threshold of target DNA obtained from two inclusion forming units (IFU), the PCR proved clearly more sensitive than EIA becoming positive at levels of 100 IFU and above. No interpretable results could be obtained for the IIF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02111875
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  • 4
    Electronic Resource
    Electronic Resource
    Occurrence of campylobacter pyloridis in patients from Vienna with gastritis and peptic ulcers (1986)
    Springer
    Infection 14 (1986), S. 275-278 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei insgesamt 120 Patienten wurden im Rahmen routinemäßiger gastroduodenoskopischer Untersuchungen Schleimhautbiopsien zur Kultivierung vonCampylobacter pyloridis entnommen. Bei sechs von sieben Patienten mit Ulcus ventriculi, 14 von 15 mit Ulcus duodeni, 15 von 20 mit Erosionen der Magenschleimhaut, 31 von 61 mit Gastritis und fünf von fünf mit Duodenitis konnteC. pyloridis isoliert werden. Hingegen war der Keimnachweis bei allen 12 Patienten mit histologisch unauffälliger Mukosa nicht möglich. Bei chronisch aktiver Gastritis ließ sichC. pyloridis signifikant häufiger als bei chronisch inaktiver Gastritis nachweisen (100 bzw. 50%). Diese Ergebnisse stützen die Hypothese vonMarshall undWarren, die besagt, daßC. pyloridis möglicherweise eine wichtige Rolle in der Entstehung von Gastritis und Ulcus pepticum zukommt.
    Notes: Summary During routine gastroduodenoscopic examination of 120 patients, biopsies of gastric mucosa were taken for the isolation ofCampylobacter pyloridis. The organism was isolated from six of seven patients with ulcus ventriculi, 14 of 15 with ulcus duodeni, 15 of 20 with erosions of the gastric mucosa, 31 of 61 with gastritis and five of five with duodenitis. In contrast, the cultures were negative in all of the 12 patients with histologically normal mucosa.C. pyloridis was isolated significantly more frequently from patients with active chronic gastritis than from those with inactive chronic gastritis (100% vs. 50%). These results support the suggestion ofMarshall andWarren that this bacterium may play an important role in the development of gastritis and peptic ulcer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01643961
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  • 5
    Electronic Resource
    Electronic Resource
    Infections following tickbites. Tick-borne encephalitis and lyme borreliosis — A prospective epidemiological study from tyrol (1988)
    Springer
    Infection 16 (1988), S. 269-272 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Ergebnisse einer prospektiven Studie über Infektionen nach Zeckenbiß bei Rekruten des österreichischen Bundesheeres im Bundesland Tirol (Österreich) werden hier vorgelegt. Insgesamt 84 Rekruten wurden nach einem Zeckenbiß zweimal innerhalb von vier Wochen klinisch und serologisch auf Zeichen einer Frühsommer-Meningo-Enzephalitis(FSME)-Virusinfektion und einerBorrelia burgdorferi-Infektion untersucht. Endgültig konnten 56 und 50 Rekruten für die Analyse von FSME-Virus- bzw. Borrelia-Infektionen herangezogen werden. Während bei keinem Rekruten eine FSME-Virusinfektion nachgewiesen werden konnte, zeigten zwei (4%) ein Erythema chronicum migrans als Primärmanifestation der Lyme-Borreliose und bei 11 (20%) Rekruten wurde ein signifikanter Anstieg von Anti-Borrelia burgdorferi-Antikörpern festgestellt. Diese Ergebnisse zeigen ein Überwiegen von subklinischen Verläufen der von Zecken übertragenen Borrelien-Infektion und erhellen die epidemiologische Situation der Zecken-vermittelten Erkrankungen in Tirol.
    Notes: Summary We present here a prospective study on infections following tickbites in military recruits in the province of Tyrol (Austria). 84 recruits experienced tickbites and underwent clinical and serological examination twice at four-week intervals for signs of tick borne encephalitis (TBE)-virus orBorrelia burgdorferi infections. 56 and 50 recruits could be evaluated for TBE-virus and Borrelia infection, respectively. Whereas no recruit was found with clinical or laboratory evidence of TBE-virus infection, two (4%) recruits showed an erythema chronicum migrans as primary manifestation of aBorrelia burgdorferi infection and 11 (20%) recruits had a significant increase in the titer of anti-Borrelia burgdorferi antibodies. Our results support the predominance of a subclinical course of a tick-transmitted borrelia infection in the population under observation, and shed some light on the epidemiological situation of tick-transmitted diseases in Tyrol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01645068
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  • 6
    Electronic Resource
    Electronic Resource
    Agglutination test versus ELISA in the diagnosis of typhoid fever (1988)
    Springer
    European journal of epidemiology 4 (1988), S. 127-128 
    Details
    ISSN: 1573-7284
    Keywords: Typhoid fever ; Agglutination test ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152706
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of a commercial latex test for serological diagnosis ofHelicobacter pylori infection in treated and untreated patients (1991)
    Springer
    European journal of clinical microbiology & infectious diseases 10 (1991), S. 971-974 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The value of a commercially available latex test (Pyloriset) for the diagnosis ofHelicobacter pylori infection by demonstration of specific antibodies was compared with that of direct diagnostic methods such as culture, biopsy-urease test and microscopy of fuchsin-stained smears. The sera were from 136 patients who prior to this study either had or had not been treated forHelicobacter pylori-infection simultaneously with amoxicillin (3 × 750 mg/day) and metronidazole (3 × 500 mg/day) for 12 days. On average, the sensitivity of the test was 90 %. The specificity with sera from untreated patients was 75.9 %; with sera from treated patients specificity was 22.2 %, 28 % and 20 % 1, 3 and 6 months respectively after start of treatment. Only as late as one year after the onset of chemotherapy did the specificity return to 67 %. Because of its low specificity this test does not offer any advantage over other tests in the detection ofHelicobacter pylori-infection or in monitoring the chemotherapeutic success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02005457
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  • 8
    Electronic Resource
    Electronic Resource
    Helicobacter pylori Type I Strains Among Austrian and Portuguese Patients with Gastritis, Peptic Ulcer or Gastric Cancer (1999)
    Springer
    European journal of clinical microbiology & infectious diseases 18 (1999), S. 807-810 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The frequency of occurrence of Helicobacter pylori type I strains in isolates from Austria and Portugal and different polymerase chain reaction-based approaches to detect the cag pathogenicity island were assessed. Of the 41 Austrian strains, eight of 14 (57.2%) isolated from patients with gastritis, 14 of 19 (73.7%) from patients with peptic ulcer and eight of eight (100%) from patients with gastric cancer were type I strains. Among the Portuguese strains, eight of 14 (57.2%) isolated from patients with gastritis, ten of 12 (83.3%) from patients with peptic ulcer and five of 13 (38.5%) from patients with gastric cancer were classified as type I. Thus, Helicobacter pylori type I strains occur frequently in both populations but show no significant correlation with peptic ulcer disease. The prevalence of the type I genotype in Austrian cancer patients, however, was significantly higher (P=0.007). The cagE-specific polymerase chain reaction was found to be a reliable and efficient method for detection of the cag pathogenicity island.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100960050405
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation and Continuous Growth of Chlamydia pneumoniae from Arterectomy Specimens (2000)
    Springer
    European journal of clinical microbiology & infectious diseases 19 (2000), S. 305-308 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  For the purpose of collecting Chlamydia pneumoniae strains of vascular origin that could be grown continuously in vitro, a cell culture system has been established. Using different types of vascular specimens obtained from 38 patients, Chlamydia pneumoniae could be isolated in three (7.9%) cases. The strains were obtained from specimens of the carotid artery, the femoral artery and an infrarenal aneurysm of the abdominal aorta of three male atherosclerosis patients. Thus, viable Chlamydia pneumoniae strains are also present in vascular regions other than those hitherto described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100960050481
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular typing ofAcinetobacter baumannii from ten different intensive care units of a university hospital (1997)
    Springer
    European journal of clinical microbiology & infectious diseases 16 (1997), S. 740-743 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Thirty-one isolates ofAcinetobacter baumannii were collected from ten intensive care units of an Austrian university hospital. All isolates were typed by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Two strains colonizing 13 infants in the neonatal intensive care unit were identified by ERIC-PCR. All otherAcinetobacter baumannii isolates had highly divergent ERIC-PCR patterns, despite having the same antibiogram. Thus, a hospital-wide clonal distribution, as suggested by identical antibiogram patterns, was excluded by ERIC-PCR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01709255
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