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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1981), S. 288-293 
    ISSN: 1432-072X
    Keywords: Clostridium aceticum ; Acetic acid formation ; Chemolithotrophic growth ; Hydrogen uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spores of Clostridium aceticum (Wieringa) from 1947 have been revived, and a study for a redescription of this microorganism has been carried out. C. aceticum was Gram negative. The cells were rodshaped and peritrichously flagellated. Round spores were formed in terminal position. The DNA contained 33 mol % guanine plus cytosine. The organism was obligately anaerobic and grew either chemolithotrophically with H2+CO2 or chemoorgranotrophically with compounds such as fructose, l-glutamate, l-malate or pyruvate. H2 and CO2 were converted to acetic acid according to the following equation: $$2{\text{ CO}}_{\text{2}} + 4{\text{H}}_{\text{2}} \to {\text{CH}}_{\text{3}} {\text{COOH + 2H}}_{\text{2}} {\text{O}}$$ The optimal temperature for growth was 30°C. The optimal pH for chemolithotrophic growth was 8.3. The doubling times for chemolithotrophic and chemoorganotrophic growth were 25 and 8h, respectively.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1981), S. 294-298 
    ISSN: 1432-072X
    Keywords: Acetobacterium woodii ; Clostridium aceticum ; Mixotrophic growth ; H2 utilization ; CO2 fixation ; H2 inhibition of chemoorganotrophic growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During growth of Acetobacterium woodii on fructose, glucose or lactate in a medium containing less than 0.04% bicarbonate, molecular hydrogen was evolved up to 0.1 mol per mol of substrate. Under an H2-atmosphere growth of A. woodii with organic substrates was completely inhibited whereas under an H2/CO2-atmosphere rapid growth occurred. Under these conditions H2+CO2 and the organic substrate were utilized simultaneously indicating that A. woodii was able to grow mixotrophically. Clostridium aceticum differed from A. woodii in that H2 was only evolved in the stationary phase, that the inhibition by H2 was observed at pH 8.5 but not at pH 7.5, anf that in the presence of fructose and H2+CO2 only fructose was utilized. The hydrogenase activity of fructose-grown cells of C. aceticum amounted to only 12% of that of H2+CO2-grown cells. With A. woodii a corresponding decrease of the activity of this enzyme was not observed.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 56 (1980), S. 71-79 
    ISSN: 1432-2242
    Keywords: Gene-ecology ; Fasciated mutants ; Penetrance ; Flowering behaviour ; Seed production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six mutants and nine recombinants of Pisum sativum were grown along with the mother variety at Kurukshetra, North India. The findings obtained were compared with those obtained for the same material grown at Bonn, Germany. The following observations were made. Stem length and degree of branching are influenced differentially in the various genotypes tested in India as a consequence of a specific reaction of the genes to the climatic conditions. A gene for weak stem fasciation and gene efr for earliness in a specific gene combination are unable to express their action in North India whereas they are fully active in Germany. Furthermore, in Kurukshetra early flowering of some recombinants does not result in early ripening because their seeds require about double the time for full ripening than those of the mother variety. At Kurukshetra, recombinant R 674A proved to be highly heat susceptible. All the plants died in early stages of ontogenetic development. Four other genotypes died due to heat before completing seed ripening. One mutant and three recombinants were found to be more tolerant to powdery mildew attack than the mother variety and Indian local lines. The seed production of eight genotypes in relation to that of the initial line was essentially better in North India than in Germany. They are obviously better adapted to the semi-arid conditions. Some of them appear to be useful for pea breeding in India. In contrast, a fasciated mutant, high yielding in Germany, is not able to express this potentiality at Kurukshetra. At Udaipur (Rajasthan, Western India), this mutant is unable to flower. Another four genotypes, tested at both Indian locations, exhibited an essentially poorer seed production at Udaipur than at Kurukshetra due to some ecological factors. The findings indicate a specific response of some of the genotypes tested to the specific ecological conditions of the three locations, their response differing from that of the mother variety demonstrating thereby a different adaptational optimum.
    Type of Medium: Electronic Resource
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